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UniProtKB - Q9T1V4 (BP45_BPMU)
Protein
Baseplate puncturing device gp45
Gene
Mup45
Organism
Escherichia phage Mu (Bacteriophage Mu)
Status
Functioni
Component of the baseplate that forms a central needlelike spike used to puncture the host cell membrane for tube insertion during virus entry. Probably involved in baseplate and tail assembly. Serves as the distal plug of tail tube channel and might regulate the process of the phage DNA and protein ejection into the host cell.
2 PublicationsCaution
Translation initiates from a non-canonical start codon (GUG).Curated
Cofactori
Protein has several cofactor binding sites:- Ca2+1 PublicationNote: Binds 1 Ca2+ cation per trimer. Ca2+ plays an important role in interaction with the host cell membrane.1 Publication
- chloride1 PublicationNote: Binds 1 Cl- ion per trimer.1 Publication
- Fe cation1 PublicationNote: Binds 1 Fe cation per trimer.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 183 | Iron; via tele nitrogen; shared with trimeric partners | 1 | |
Metal bindingi | 185 | Iron; via tele nitrogen; shared with trimeric partners | 1 | |
Metal bindingi | 188 | Calcium; shared with trimeric partners | 1 | |
Binding sitei | 188 | Chloride; via amide nitrogen; shared with trimeric partners | 1 | |
Metal bindingi | 189 | Calcium; shared with trimeric partners | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- viral genome ejection through host cell envelope, contractile tail mechanism Source: UniProtKB-KW
- viral tail assembly Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Baseplate puncturing device gp45Alternative name(s): Baseplate spike protein Gene product 45 Short name: gp45 Gene product Q Short name: gpQ |
Gene namesi | Ordered Locus Names:Mup45 |
Organismi | Escherichia phage Mu (Bacteriophage Mu) |
Taxonomic identifieri | 10677 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Myoviridae › Muvirus |
Virus hosti | Enterobacteriaceae [TaxID: 543] |
Proteomesi |
|
Subcellular locationi
- Virion 1 Publication
- Host cytoplasm Curated Note: Baseplate protein.1 Publication
Keywords - Cellular componenti
Host cytoplasm, Viral baseplate protein, Viral tail protein, VirionPathology & Biotechi
Disruption phenotypei
No tail is synthesized.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 188 | D → A: Loss of membrane-binding ability. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000077838 | 1 – 197 | Baseplate puncturing device gp45Add BLAST | 197 |
Expressioni
Inductioni
Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication
Keywords - Developmental stagei
Late proteinInteractioni
Subunit structurei
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9T1V4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 172 – 197 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 177 – 197 | Ions bindingAdd BLAST | 21 |
Domaini
The C-terminus is a needlelike shaped homotrimer consisting of an intertwined beta-sheet, a triple beta-helix and a metal-binding region.1 Publication
Family and domain databases
InterProi | View protein in InterPro IPR013046, GpV/Gp45 IPR014462, Phage_Mu_Gp45 IPR040629, Phage_spike |
Pfami | View protein in Pfam PF06890, Phage_Mu_Gp45, 1 hit PF18715, Phage_spike, 1 hit |
PIRSFi | PIRSF012337, gp45, 1 hit |
TIGRFAMsi | TIGR01644, phage_P2_V, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9T1V4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERVNDSALN RLLTPLMRRV RLMLARAVVN VINDGRKVQN LQVGLLDDEE
60 70 80 90 100
SDEVERLQNY GHFSVPLPGA EALIACVGAQ RDQGIAVVVE DRRYRPTNLE
110 120 130 140 150
PGDAGIYHHE GHRIRLTKDG RCIITCKTVE VYADESMTVD TPRTTFTGDV
160 170 180 190
EIQKGLGVKG KSQFDSNITA PDAIINGKST DKHIHRGDSG GTTGPMQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083977 Genomic DNA Translation: AAF01123.1 |
RefSeqi | NP_050649.1, NC_000929.1 |
Genome annotation databases
GeneIDi | 2636280 |
KEGGi | vg:2636280 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083977 Genomic DNA Translation: AAF01123.1 |
RefSeqi | NP_050649.1, NC_000929.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3VTN | X-ray | 1.75 | A | 100-197 | [»] | |
3VTO | X-ray | 1.44 | A/B/C/P/Q/R | 92-197 | [»] | |
SMRi | Q9T1V4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
GeneIDi | 2636280 |
KEGGi | vg:2636280 |
Family and domain databases
InterProi | View protein in InterPro IPR013046, GpV/Gp45 IPR014462, Phage_Mu_Gp45 IPR040629, Phage_spike |
Pfami | View protein in Pfam PF06890, Phage_Mu_Gp45, 1 hit PF18715, Phage_spike, 1 hit |
PIRSFi | PIRSF012337, gp45, 1 hit |
TIGRFAMsi | TIGR01644, phage_P2_V, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BP45_BPMU | |
Accessioni | Q9T1V4Primary (citable) accession number: Q9T1V4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 70 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references