Mup45

Functionⁱ

Component of the baseplate that forms a central needlelike spike used to puncture the host cell membrane for tube insertion during virus entry. Probably involved in baseplate and tail assembly. Serves as the distal plug of tail tube channel and might regulate the process of the phage DNA and protein ejection into the host cell.2 Publications

Caution

Translation initiates from a non-canonical start codon (GUG).Curated

Cofactorⁱ

Protein has several cofactor binding sites:

Ca²⁺
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
  Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
  Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.
Note: Binds 1 Ca²⁺ cation per trimer. Ca²⁺ plays an important role in interaction with the host cell membrane.
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
  Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
  Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.
chloride
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
  Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
  Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.
Note: Binds 1 Cl^- ion per trimer.
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
  Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
  Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.
Fe cation
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
  Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
  Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.
Note: Binds 1 Fe cation per trimer.
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion."
  Harada K., Yamashita E., Nakagawa A., Miyafusa T., Tsumoto K., Ueno T., Toyama Y., Takeda S.
  Biochim. Biophys. Acta 1834:284-291(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.44 ANGSTROMS) OF 92-197, FUNCTION, SUBUNIT, DOMAIN, COFACTOR, MUTAGENESIS OF ASP-188.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	183	Iron; via tele nitrogen; shared with trimeric partners	1
Metal bindingⁱ	185	Iron; via tele nitrogen; shared with trimeric partners	1
Metal bindingⁱ	188	Calcium; shared with trimeric partners	1
Binding siteⁱ	188	Chloride; via amide nitrogen; shared with trimeric partners	1
Metal bindingⁱ	189	Calcium; shared with trimeric partners	1

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

viral genome ejection through host cell envelope, contractile tail mechanism Source: UniProtKB-KW
viral tail assembly Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Viral contractile tail ejection system
Biological process	Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Viral release from host cell, Viral tail assembly, Virus entry into host cell
Ligand	Calcium, Chloride, Iron, Metal-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Baseplate puncturing device gp45 Alternative name(s): Baseplate spike protein Gene product 45 Short name: gp45 Gene product Q Short name: gpQ
Gene namesⁱ	Ordered Locus Names:Mup45
Organismⁱ	Escherichia phage Mu (Bacteriophage Mu)
Taxonomic identifierⁱ	10677 [NCBI]
Taxonomic lineageⁱ	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Muvirus
Virus hostⁱ	Enterobacteriaceae [TaxID: 543]
Proteomesⁱ	UP000002611 Componentⁱ: Genome

Subcellular locationⁱ

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell
virus tail, baseplate Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm, Viral baseplate protein, Viral tail protein, Virion

Pathology & Biotechⁱ

Disruption phenotypeⁱ

No tail is synthesized.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	188	D → A: Loss of membrane-binding ability. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000077838	1 – 197	Baseplate puncturing device gp45Add BLAST		197

Expressionⁱ

Inductionⁱ

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stageⁱ

Late protein

Interactionⁱ

Subunit structureⁱ

Homotrimer (PubMed:20478417, PubMed:22922659). Part of a complex composed of three DNA circularization protein N, three baseplate hub protein gp44 and three sub-complex wedge (made of two copies of each baseplate protein gp46, gp47 and gp48) that forms the baseplate (PubMed:27555589).3 Publications

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	104 – 107	Combined sources	4
Beta strandⁱ	113 – 117	Combined sources	5
Turnⁱ	118 – 120	Combined sources	3
Beta strandⁱ	121 – 140	Combined sources	20
Beta strandⁱ	142 – 154	Combined sources	13
Beta strandⁱ	156 – 160	Combined sources	5
Beta strandⁱ	162 – 166	Combined sources	5
Beta strandⁱ	168 – 171	Combined sources	4
Beta strandⁱ	173 – 175	Combined sources	3
Turnⁱ	180 – 182	Combined sources	3
Beta strandⁱ	190 – 192	Combined sources	3

Show more details

3D structure databases

SMRⁱ	Q9T1V4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	177 – 197	Ions bindingAdd BLAST		21

Domainⁱ

The C-terminus is a needlelike shaped homotrimer consisting of an intertwined beta-sheet, a triple beta-helix and a metal-binding region.1 Publication

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG090000U1

OrthoDBⁱ

VOG090000U1

Family and domain databases

InterProⁱ	View protein in InterPro IPR013046 GpV/Gp45 IPR014462 Phage_Mu_Gp45
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06890 Phage_Mu_Gp45, 1 hit
PIRSFⁱ	PIRSF012337 gp45, 1 hit
TIGRFAMsⁱ	TIGR01644 phage_P2_V, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9T1V4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MERVNDSALN RLLTPLMRRV RLMLARAVVN VINDGRKVQN LQVGLLDDEE 
        60         70         80         90        100
SDEVERLQNY GHFSVPLPGA EALIACVGAQ RDQGIAVVVE DRRYRPTNLE 
       110        120        130        140        150
PGDAGIYHHE GHRIRLTKDG RCIITCKTVE VYADESMTVD TPRTTFTGDV 
       160        170        180        190 
EIQKGLGVKG KSQFDSNITA PDAIINGKST DKHIHRGDSG GTTGPMQ

Length:197

Mass (Da):21,689

Last modified:May 1, 2000 - v1

Checksum:ⁱ448C7A7ADCADD5C9

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF083977 Genomic DNA Translation: AAF01123.1
NCBI Reference Sequences More...RefSeqⁱ	NP_050649.1, NC_000929.1

Genome annotation databases

GeneIDⁱ	2636280
KEGGⁱ	vg:2636280

Protein	Similar proteins		Species	Score	Length	Source
Q9T1V4	Uncharacterized protein		Salmonella enterica subsp. enterica serovar Montevideo str. USDA-ARS-USMARC-1900		197	UniRef100_Q9T1V4
Phage baseplate assembly protein V		ECOLX		197
Phage baseplate assembly protein V		ECOLW		197
Phage baseplate assembly protein V		ECO24		197
Phage baseplate assembly protein V		ECOLX		181
+3

Protein	Similar proteins		Species	Score	Length	Source
Q9T1V4	Uncharacterized protein		Salmonella enterica subsp. enterica serovar Montevideo str. USDA-ARS-USMARC-1900		197	UniRef90_Q9T1V4
Phage baseplate assembly protein V		ECOLX		197
Phage baseplate assembly protein V		ECOLW		197
Phage baseplate assembly protein V		ECO24		197
Hypothetical phage protein		ECOSE		197
+52

Protein	Similar proteins		Species	Score	Length	Source
Q9T1V4	Uncharacterized protein		Salmonella enterica subsp. enterica serovar Montevideo str. USDA-ARS-USMARC-1900		197	UniRef50_Q9T1V4
Phage baseplate assembly protein V		ECOLX		197
Phage baseplate assembly protein V		ECOLW		197
Phage baseplate assembly protein V		ECO24		197
Bacteriophage Mu Gp45 protein		Escherichia coli TA447		197
+156

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF083977 Genomic DNA Translation: AAF01123.1
RefSeqⁱ	NP_050649.1, NC_000929.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3VTN	X-ray	1.75	A	100-197	[»]
	3VTO	X-ray	1.44	A/B/C/P/Q/R	92-197	[»]
SMRⁱ	Q9T1V4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	2636280
KEGGⁱ	vg:2636280

Phylogenomic databases

OrthoDBⁱ	VOG090000U1

OrthoDBⁱ

VOG090000U1

Family and domain databases

InterProⁱ	View protein in InterPro IPR013046 GpV/Gp45 IPR014462 Phage_Mu_Gp45
Pfamⁱ	View protein in Pfam PF06890 Phage_Mu_Gp45, 1 hit
PIRSFⁱ	PIRSF012337 gp45, 1 hit
TIGRFAMsⁱ	TIGR01644 phage_P2_V, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BP45_BPMU
Accessionⁱ	Q9T1V4Primary (citable) accession number: Q9T1V4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	May 1, 2000
	Last modified:	November 22, 2017
	This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9T1V4 (BP45_BPMU)

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Baseplate puncturing device gp45

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Keywords - Developmental stagei

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Keywords - Developmental stageⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ