UniProtKB - Q9T0N8 (CKX1_MAIZE)
Protein
Cytokinin dehydrogenase 1
Gene
CKX1
Organism
Zea mays (Maize)
Status
Functioni
Catalyzes the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.
Catalytic activityi
- EC:1.5.99.12
Cofactori
Activity regulationi
Competitive inhibition by phenylureas.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 110 | FAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 169 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 174 | FADBy similarity | 1 | |
Binding sitei | 235 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 491 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 100 – 104 | FADBy similarity | 5 | |
Nucleotide bindingi | 105 – 106 | FADBy similarity | 2 | |
Nucleotide bindingi | 180 – 184 | FADBy similarity | 5 | |
Nucleotide bindingi | 527 – 530 | FADBy similarity | 4 |
GO - Molecular functioni
- cytokinin dehydrogenase activity Source: UniProtKB-EC
- FAD binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- cytokinin metabolic process Source: InterPro
- oxidation-reduction process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | MetaCyc:CKX1-MONOMER |
BRENDAi | 1.5.99.12, 6752 |
SABIO-RKi | Q9T0N8 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytokinin dehydrogenase 1 (EC:1.5.99.12)Alternative name(s): Cytokinin oxidase 1 Short name: CKO 1 Short name: COX 1 ZmCKX1 |
Gene namesi | Name:CKX1 |
Organismi | Zea mays (Maize) |
Taxonomic identifieri | 4577 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogonodae › Andropogoneae › Tripsacinae › Zea |
Proteomesi |
|
Organism-specific databases
MaizeGDBi | 194080 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000020424 | 19 – 534 | Cytokinin dehydrogenase 1Add BLAST | 516 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 52 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 63 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 105 | Pros-8alpha-FAD histidine | 1 | |
Glycosylationi | 134 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 294 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 323 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 338 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 434 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylated; with approximately 10 hexose residues per site.
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9T0N8 |
PRIDEi | Q9T0N8 |
Expressioni
Tissue specificityi
Expressed in immature kernels and unpollinated cobs. Weakly expressed in kernels harvested two weeks after anthesis.1 Publication
Gene expression databases
ExpressionAtlasi | Q9T0N8, baseline and differential |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 4577.GRMZM2G146644_P01 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9T0N8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9T0N8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 65 – 245 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 181 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 339 – 345 | Poly-Ala | 7 |
Sequence similaritiesi
Belongs to the oxygen-dependent FAD-linked oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1231, Eukaryota |
OrthoDBi | 350817at2759 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9T0N8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVVYYLLLA GLIACSHALA AGTPALGDDR GRPWPASLAA LALDGKLRTD
60 70 80 90 100
SNATAAASTD FGNITSALPA AVLYPSSTGD LVALLSAANS TPGWPYTIAF
110 120 130 140 150
RGRGHSLMGQ AFAPGGVVVN MASLGDAAAP PRINVSADGR YVDAGGEQVW
160 170 180 190 200
IDVLRASLAR GVAPRSWNDY LYLTVGGTLS NAGISGQAFR HGPQISNVLE
210 220 230 240 250
MDVITGHGEM VTCSKQLNAD LFDAVLGGLG QFGVITRARI AVEPAPARAR
260 270 280 290 300
WVRFVYTDFA AFSADQERLT APRPGGGGAS FGPMSYVEGS VFVNQSLATD
310 320 330 340 350
LANTGFFTDA DVARIVALAG ERNATTVYSI EATLNYDNAT AAAAAVDQEL
360 370 380 390 400
ASVLGTLSYV EGFAFQRDVA YAAFLDRVHG EEVALNKLGL WRVPHPWLNM
410 420 430 440 450
FVPRSRIADF DRGVFKGILQ GTDIVGPLIV YPLNKSMWDD GMSAATPSED
460 470 480 490 500
VFYAVSLLFS SVAPNDLARL QEQNRRILRF CDLAGIQYKT YLARHTDRSD
510 520 530
WVRHFGAAKW NRFVEMKNKY DPKRLLSPGQ DIFN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | G → A in CAA77151 (PubMed:10230061).Curated | 1 | |
Sequence conflicti | 168 | N → T in CAA77151 (PubMed:10230061).Curated | 1 | |
Sequence conflicti | 254 | F → L in CAA77151 (PubMed:10230061).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y18377 mRNA Translation: CAA77151.1 AF044603 Genomic DNA Translation: AAC27500.1 |
PIRi | T01500 T51929 |
RefSeqi | NP_001105591.1, NM_001112121.1 |
Genome annotation databases
GeneIDi | 542585 |
KEGGi | zma:542585 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y18377 mRNA Translation: CAA77151.1 AF044603 Genomic DNA Translation: AAC27500.1 |
PIRi | T01500 T51929 |
RefSeqi | NP_001105591.1, NM_001112121.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1W1O | X-ray | 1.70 | A | 1-534 | [»] | |
1W1Q | X-ray | 1.80 | A | 1-534 | [»] | |
1W1R | X-ray | 1.90 | A | 1-534 | [»] | |
1W1S | X-ray | 2.00 | A | 1-534 | [»] | |
2QKN | X-ray | 2.15 | A | 19-534 | [»] | |
2QPM | X-ray | 1.85 | A | 19-534 | [»] | |
3BW7 | X-ray | 1.95 | A | 19-534 | [»] | |
3C0P | X-ray | 1.95 | A | 19-534 | [»] | |
3DQ0 | X-ray | 1.90 | A | 19-534 | [»] | |
3KJM | X-ray | 1.90 | A | 19-534 | [»] | |
3S1C | X-ray | 2.09 | A | 19-534 | [»] | |
3S1D | X-ray | 1.75 | A | 19-534 | [»] | |
3S1E | X-ray | 1.90 | A | 19-534 | [»] | |
3S1F | X-ray | 2.00 | A | 19-534 | [»] | |
SMRi | Q9T0N8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 4577.GRMZM2G146644_P01 |
Chemistry databases
ChEMBLi | CHEMBL5363 |
Proteomic databases
PaxDbi | Q9T0N8 |
PRIDEi | Q9T0N8 |
Genome annotation databases
GeneIDi | 542585 |
KEGGi | zma:542585 |
Organism-specific databases
MaizeGDBi | 194080 |
Phylogenomic databases
eggNOGi | KOG1231, Eukaryota |
OrthoDBi | 350817at2759 |
Enzyme and pathway databases
BioCyci | MetaCyc:CKX1-MONOMER |
BRENDAi | 1.5.99.12, 6752 |
SABIO-RKi | Q9T0N8 |
Miscellaneous databases
EvolutionaryTracei | Q9T0N8 |
PROi | PR:Q9T0N8 |
Gene expression databases
ExpressionAtlasi | Q9T0N8, baseline and differential |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N IPR006093, Oxy_OxRdtase_FAD_BS |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit PS00862, OX2_COVAL_FAD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CKX1_MAIZE | |
Accessioni | Q9T0N8Primary (citable) accession number: Q9T0N8 Secondary accession number(s): O81158 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2002 |
Last sequence update: | May 24, 2005 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families