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Protein

Folate synthesis bifunctional protein, mitochondrial

Gene

MitHPPK/DHPS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis.By similarity

Catalytic activityi

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.By similarity
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Dihydroneopterin aldolase 2 (FOLB2), 7,8-dihydroneopterin aldolase (AXX17_At5g62530), 7,8-dihydroneopterin aldolase (AXX17_At3g23310), 7,8-dihydroneopterin aldolase (AXX17_At3g11690), Probable dihydroneopterin aldolase 3 (FOLB3), Dihydroneopterin aldolase 1 (FOLB1), 7,8-dihydroneopterin aldolase (AXX17_At3g11690)
  4. Folate synthesis bifunctional protein (CytHPPK/DHPS), Folate synthesis bifunctional protein, mitochondrial (MitHPPK/DHPS)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.Curated
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Folate synthesis bifunctional protein (CytHPPK/DHPS), Folate synthesis bifunctional protein, mitochondrial (MitHPPK/DHPS)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi280MagnesiumBy similarity1
Binding sitei3206-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei3576-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei3766-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei4496-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei4946-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Multifunctional enzyme, Transferase
Biological processFolate biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G30000-MONOMER.
UniPathwayiUPA00077; UER00155.
UPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Folate synthesis bifunctional protein, mitochondrial1 Publication
Including the following 2 domains:
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase1 Publication (EC:2.7.6.3By similarity)
Short name:
HPPK1 Publication
Alternative name(s):
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Dihydropteroate synthase1 Publication (EC:2.5.1.15By similarity)
Short name:
DHPS1 Publication
Gene namesi
Name:MitHPPK/DHPS1 Publication
Ordered Locus Names:At4g30000Imported
ORF Names:F6G3.30Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G30000.
TAIRilocus:2126465. AT4G30000.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionSequence analysisAdd BLAST42
ChainiPRO_000043286843 – 554Folate synthesis bifunctional protein, mitochondrialAdd BLAST512

Proteomic databases

PaxDbiQ9SZV3.
PRIDEiQ9SZV3.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

Not induced by salt stress.1 Publication

Gene expression databases

ExpressionAtlasiQ9SZV3. baseline and differential.
GenevisibleiQ9SZV3. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G30000.2.

Structurei

3D structure databases

ProteinModelPortaliQ9SZV3.
SMRiQ9SZV3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini273 – 541Pterin-bindingPROSITE-ProRule annotationAdd BLAST269

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 215HPPKCuratedAdd BLAST126
Regioni275 – 554DHPSAdd BLAST280
Regioni529 – 5316-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi548 – 551Poly-ArgSequence analysis4

Sequence similaritiesi

In the N-terminal section; belongs to the HPPK family.Curated
In the C-terminal section; belongs to the DHPS family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2544. Eukaryota.
COG0294. LUCA.
COG0801. LUCA.
HOGENOMiHOG000217511.
InParanoidiF4JPH1.
KOiK13941.
OMAiSLWETEP.
OrthoDBiEOG093607KB.
PhylomeDBiQ9SZV3.

Family and domain databases

CDDicd00739. DHPS. 1 hit.
cd00483. HPPK. 1 hit.
Gene3Di3.20.20.20. 1 hit.
3.30.70.560. 1 hit.
InterProiView protein in InterPro
IPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000550. Hppk.
IPR035907. Hppk_sf.
IPR000489. Pterin-binding_dom.
PfamiView protein in Pfam
PF01288. HPPK. 1 hit.
PF00809. Pterin_bind. 1 hit.
SUPFAMiSSF51717. SSF51717. 1 hit.
SSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
TIGR01498. folK. 1 hit.
PROSITEiView protein in PROSITE
PS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS00794. HPPK. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SZV3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPLLSQTLI HTGRFLLRRF LEPPPAVISA VAASRVCFHR YYSSKSLSLV
60 70 80 90 100
SPLGLHCSSL FSPPALCNSA FSSSATSTTI EVQSTEHEVV IALGSNIGNR
110 120 130 140 150
MNNFREALRL MKRGGICVTR HSCLYETAPV HVTDQPRFLN AAVRGVTKLG
160 170 180 190 200
PHELLSVLKT IERDMGRKDG IRYGPRPLDL DILFYGKMRI SSDKLIIPHE
210 220 230 240 250
RLWERSFVLA PLVDLLGSAV DNDTVAHWHS LAIHPGGIFQ AWERLGGESL
260 270 280 290 300
IGQDGIQRVL PIGDKLWDFS NKTHVMGILN LTPDSFSDGG KFQSIDSAVS
310 320 330 340 350
RVRSMISEGA DIIDIGAQST RPMASRISSQ EELDRLLPVL EAVRGMPEME
360 370 380 390 400
EKLISVDTFN SEVASEAISN GADILNDVSA GTLDPNMHKV VAESGVPYMA
410 420 430 440 450
MHMRGDPCTM QNKENLQYDD VCKDVASELY LRVRDAELSG IPAWRVMIDP
460 470 480 490 500
GIGFSKSVDH NLDIIMDLPK IREEMAKRSI AVSHAPILVG PSRKRFLGDI
510 520 530 540 550
CGRPEATDRD AATVASVTAG ILGGANIIRV HNVRHNADAA KVCDAMLRRR

RSKG
Length:554
Mass (Da):60,738
Last modified:May 1, 2000 - v1
Checksum:i97732C1F17761157
GO
Isoform 2 (identifier: Q9SZV3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     542-554: VCDAMLRRRRSKG → IDTAVSLCKNNMPREATFLL

Note: No experimental confirmation available. Derived from EST data.Curated
Show »
Length:561
Mass (Da):61,427
Checksum:iC943FB3BEDBD3946
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057628542 – 554VCDAM…RRSKG → IDTAVSLCKNNMPREATFLL in isoform 2. Add BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL078464 Genomic DNA. Translation: CAB43835.1.
AL161576 Genomic DNA. Translation: CAB80993.1.
CP002687 Genomic DNA. Translation: AEE85706.1.
CP002687 Genomic DNA. Translation: AEE85707.1.
BT033093 mRNA. Translation: ACF06123.1.
PIRiT08976.
RefSeqiNP_001190868.1. NM_001203939.1. [Q9SZV3-2]
NP_194729.1. NM_119146.3. [Q9SZV3-1]
UniGeneiAt.31868.

Genome annotation databases

EnsemblPlantsiAT4G30000.1; AT4G30000.1; AT4G30000. [Q9SZV3-1]
AT4G30000.2; AT4G30000.2; AT4G30000. [Q9SZV3-2]
GeneIDi829123.
GrameneiAT4G30000.1; AT4G30000.1; AT4G30000. [Q9SZV3-1]
AT4G30000.2; AT4G30000.2; AT4G30000. [Q9SZV3-2]
KEGGiath:AT4G30000.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFOLM_ARATH
AccessioniPrimary (citable) accession number: Q9SZV3
Secondary accession number(s): F4JPH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2015
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome