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Protein

Putative GATA transcription factor 22

Gene

GATA22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters (PubMed:22102866, PubMed:25077795). Involved in the modulation of chloroplast development, growth and division in a cytokinin-dependent manner (PubMed:22102866, PubMed:22811435). Repressor of the gibberellic acid (GA) signaling pathway that regulates flowering and modulates greening, in a SOC1-dependent manner (PubMed:20844019, PubMed:23739688, PubMed:25077795). Prevents the accumulation of SOC1 during flowering (PubMed:23739688). Promotes chlorophyll biosynthesis throughout the plant, by regulating chlorophyll biosynthetic genes (e.g. HEMA1 and GUN4) and chloroplast localized glutamate synthase (e.g. GLU1) (PubMed:18417639, PubMed:20844019, PubMed:21453984, PubMed:22102866, PubMed:23878229, PubMed:25077795). Involved in the regulation of sugar-sensing genes (e.g. HXK1, HXK2, STP13 and PLT6) (PubMed:18417639). Regulator of germination, senescence, elongation growth and flowering time (PubMed:20844019, PubMed:22102866, PubMed:23878229). Influences also leaf starch content (PubMed:22102866).8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri195 – 249GATA-typePROSITE-ProRule annotationAdd BLAST55

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processCytokinin signaling pathway, Gibberellin signaling pathway, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Putative GATA transcription factor 221 Publication
Alternative name(s):
Protein CYTOKININ-RESPONSIVE GATA FACTOR 11 Publication
Protein GNC-LIKE1 Publication
Short name:
AtGNL1 Publication
Gene namesi
Name:GATA221 Publication
Synonyms:CGA11 Publication, GNL1 Publication
Ordered Locus Names:At4g26150Imported
ORF Names:F20B18.260Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26150
TAIRilocus:2120845 AT4G26150

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Pale green leaves and reduced chlorophyll levels associated with altered regulation of sugar-sensing genes (e.g. HXK1, HXK2, STP13 and PLT6) (PubMed:18417639, PubMed:21453984, PubMed:22102866, PubMed:22811435). Reduced chloroplast size (PubMed:22811435). Faster seed germination. Early flowering. Increased leaves size (PubMed:20844019, PubMed:22102866). Reduced gibberellic acid (GA) levels due to increased GA turnover and associated with reduced expression of GA-anabolizing enzymes (e.g. GA3OX1) but increased expression of GA-catabolizing enzymes (e.g. GA2OX2) (PubMed:20844019). Small seeds with deformed seed coats (PubMed:22102866). The double mutant gnc cga1, lacking both GATA22 and GATA21, exhibits reduced sensitivity to cytokinin (e.g. benzyladenine) toward chloroplasts growth (PubMed:22811435).5 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi341 – 343LLM → AAA: Increased inhibition of germination by paclobutrazol (PAC), a gibberellic acid (GA) biosynthesis inhibitor. Slight reduction of hypocotyl elongation, with rounder leaves and shortened petioles. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834491 – 352Putative GATA transcription factor 22Add BLAST352

Proteomic databases

PaxDbiQ9SZI6
PRIDEiQ9SZI6

Expressioni

Tissue specificityi

Expressed predominantly in leaves, and barely in stems, flowers and siliques.4 Publications

Developmental stagei

First observed in the inflorescence meristem (IM) and young flower buds (PubMed:23335616). Detected throughout the floral bud. In young flowers, restricted to the inner whorls, specifically the petals, stamens, and carpels (PubMed:18417639, PubMed:23335616). In older flowers, accumulates more in the stamens than in the petals and carpels (PubMed:18417639). Observed in anther locules, vascular strands, and ovules (PubMed:23335616). During imbibition, expressed in the endosperm, especially at the time of testa rupture. Later restricted to the cotyledons (PubMed:20844019). In mature embryos, restricted to the cotyledons. In young seedlings, mostly expressed in shoot tissues, including the tip, circumference, and vasculature of the cotyledons, the emerging leaves, the meristematic region, and the basal part of the hypocotyl, and, at low levels, in the primary roots. In older seedlings, accumulates in the green shoot tissues (PubMed:22811435).4 Publications

Inductioni

By light (including both red and white lights) (PubMed:17208962, PubMed:17587690). Levels follow a circadian and diurnal rythm, with a peak at 20 hours, thus preempting dawn (PubMed:17208962). Activated by gibberellic acid (GA) (PubMed:20844019). Induced by cytokinin and derivatives (e.g. benzyladenine, t-Zeatin and 6-benzylaminopurine) in light conditions (PubMed:16212609, PubMed:17587690, PubMed:21453984, PubMed:22811435). Triggered by nitrate (PubMed:16262716). Negatively regulated by AP3/PI (PubMed:18417639). Strong accumulation during cold imbibition of nondormant seeds, but not at warm temperatures. Regulated by PIF transcription factors (PubMed:20844019). Repressed by HAN (PubMed:23335616). Inhibited by SOC1 (PubMed:23739688). Down-regulated by auxin (2,4D) and auxin response factors (e.g. ARF2 and ARF7) (PubMed:23878229).1 Publication10 Publications

Gene expression databases

ExpressionAtlasiQ9SZI6 differential
GenevisibleiQ9SZI6 AT

Interactioni

Subunit structurei

Forms heterodimers with GATA18.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9SZI6, 4 interactors
STRINGi3702.AT4G26150.1

Structurei

3D structure databases

ProteinModelPortaliQ9SZI6
SMRiQ9SZI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi112 – 119Nuclear localization signalPROSITE-ProRule annotation8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 40Poly-Gln8

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri195 – 249GATA-typePROSITE-ProRule annotationAdd BLAST55

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1601 Eukaryota
COG5641 LUCA
HOGENOMiHOG000238913
InParanoidiQ9SZI6
OMAiNDCVIRI
OrthoDBiEOG09360JYV
PhylomeDBiQ9SZI6

Family and domain databases

CDDicd00202 ZnF_GATA, 1 hit
Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR039355 Transcription_factor_GATA
IPR000679 Znf_GATA
IPR013088 Znf_NHR/GATA
PANTHERiPTHR10071 PTHR10071, 1 hit
PfamiView protein in Pfam
PF00320 GATA, 1 hit
SMARTiView protein in SMART
SM00401 ZnF_GATA, 1 hit
PROSITEiView protein in PROSITE
PS00344 GATA_ZN_FINGER_1, 1 hit
PS50114 GATA_ZN_FINGER_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SZI6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSNFHYTID LNEDQNHQPF FASLGSSLHH HLQQQQQQQQ HFHHQASSNP
60 70 80 90 100
SSLMSPSLSY FPFLINSRQD QVYVGYNNNT FHDVLDTHIS QPLETKNFVS
110 120 130 140 150
DGGSSSSDQM VPKKETRLKL TIKKKDNHQD QTDLPQSPIK DMTGTNSLKW
160 170 180 190 200
ISSKVRLMKK KKAIITTSDS SKQHTNNDQS SNLSNSERQN GYNNDCVIRI
210 220 230 240 250
CSDCNTTKTP LWRSGPRGPK SLCNACGIRQ RKARRAAMAT ATATAVSGVS
260 270 280 290 300
PPVMKKKMQN KNKISNGVYK ILSPLPLKVN TCKRMITLEE TALAEDLETQ
310 320 330 340 350
SNSTMLSSSD NIYFDDLALL LSKSSAYQQV FPQDEKEAAI LLMALSHGMV

HG
Length:352
Mass (Da):39,345
Last modified:May 1, 2000 - v1
Checksum:i620E693D93D80904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049483 Genomic DNA Translation: CAB39680.1
AL161564 Genomic DNA Translation: CAB79470.1
CP002687 Genomic DNA Translation: AEE85164.1
DQ875131 mRNA Translation: ABL73201.1
PIRiT04270
RefSeqiNP_194345.1, NM_118748.3
UniGeneiAt.32205

Genome annotation databases

EnsemblPlantsiAT4G26150.1; AT4G26150.1; AT4G26150
GeneIDi828721
GrameneiAT4G26150.1; AT4G26150.1; AT4G26150
KEGGiath:AT4G26150

Similar proteinsi

Entry informationi

Entry nameiGAT22_ARATH
AccessioniPrimary (citable) accession number: Q9SZI6
Secondary accession number(s): A3E4C1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 1, 2000
Last modified: September 12, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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