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Protein

Copper-transporting ATPase PAA1, chloroplastic

Gene

PAA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates copper transfer across the plastid envelope. Required for the delivery of copper into the plastid stroma, which is essential for the function of copper proteins. Seems to be selective for monovalent copper Cu+ transport. Plays also a role in glucose signaling-mediated cell proliferation of root meristem in non-green tissues.4 Publications

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi159CopperPROSITE-ProRule annotation1
Metal bindingi162CopperPROSITE-ProRule annotation1
Active sitei5984-aspartylphosphate intermediateBy similarity1
Metal bindingi808MagnesiumPROSITE-ProRule annotation1
Metal bindingi812MagnesiumPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi807 – 814ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cation-transporting ATPase activity Source: InterPro
  • copper chaperone activity Source: TAIR
  • copper ion transmembrane transporter activity Source: TAIR

GO - Biological processi

  • copper ion homeostasis Source: TAIR
  • copper ion transmembrane transport Source: UniProtKB
  • photosynthetic electron transport chain Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processCopper transport, Ion transport, Transport
LigandATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G33520-MONOMER
MetaCyc:MONOMER-14495
BRENDAi3.6.3.4 399

Protein family/group databases

TCDBi3.A.3.5.11 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-transporting ATPase PAA1, chloroplastic (EC:3.6.3.54)
Alternative name(s):
Protein HEAVY METAL ATPASE 6
Protein glucose insensitive root 1
Gene namesi
Name:PAA1
Synonyms:GIR1, HMA6
Ordered Locus Names:At4g33520
ORF Names:F17M5.280, T16L1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G33520
TAIRilocus:2119265 AT4G33520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei253 – 274HelicalSequence analysisAdd BLAST22
Transmembranei287 – 306HelicalSequence analysisAdd BLAST20
Transmembranei314 – 334HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Transmembranei502 – 524HelicalSequence analysisAdd BLAST23
Transmembranei543 – 560HelicalSequence analysisAdd BLAST18
Transmembranei863 – 882HelicalSequence analysisAdd BLAST20
Transmembranei895 – 913HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

Pathology & Biotechi

Disruption phenotypei

High-chlorophyll-fluorescence phenotype. Short roots.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 103ChloroplastSequence analysisAdd BLAST103
ChainiPRO_0000046403104 – 949Copper-transporting ATPase PAA1, chloroplasticAdd BLAST846

Proteomic databases

PaxDbiQ9SZC9
PRIDEiQ9SZC9

Expressioni

Tissue specificityi

Expressed in the shoots and roots.1 Publication

Gene expression databases

ExpressionAtlasiQ9SZC9 baseline and differential
GenevisibleiQ9SZC9 AT

Interactioni

Protein-protein interaction databases

BioGridi14774, 4 interactors
IntActiQ9SZC9, 2 interactors
STRINGi3702.AT4G33520.2

Structurei

Secondary structure

1949
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi609 – 614Combined sources6
Helixi629 – 640Combined sources12
Helixi646 – 655Combined sources10
Beta strandi671 – 673Combined sources3
Turni674 – 676Combined sources3
Beta strandi677 – 682Combined sources6
Beta strandi685 – 690Combined sources6
Helixi692 – 697Combined sources6
Helixi704 – 710Combined sources7
Beta strandi718 – 723Combined sources6
Beta strandi726 – 734Combined sources9

3D structure databases

ProteinModelPortaliQ9SZC9
SMRiQ9SZC9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 223HMAPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi115 – 124Poly-Gly10

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207 Eukaryota
COG2217 LUCA
HOGENOMiHOG000250397
InParanoidiQ9SZC9
KOiK01533
OMAiIHSTGFH
PhylomeDBiQ9SZC9

Family and domain databases

CDDicd00371 HMA, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017969 Heavy-metal-associated_CS
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 1 hit
SUPFAMiSSF55008 SSF55008, 1 hit
SSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS01047 HMA_1, 1 hit
PS50846 HMA_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SZC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESTLSAFST VKATAMARSS GGPSLPLLTI SKALNRHFTG ARHLHPLLLA
60 70 80 90 100
RCSPSVRRLG GFHGSRFTSS NSALRSLGAA VLPVIRHRLE CLSSSSPSFR
110 120 130 140 150
SISSGGGSGF GGYNGGSGGG GGGGSESGDS KSKLGANASD GVSVPSSDII
160 170 180 190 200
ILDVGGMTCG GCSASVKKIL ESQPQVASAS VNLTTETAIV WPVPEAKSVP
210 220 230 240 250
DWQKSLGETL ANHLTNCGFQ STPRDLVTEN FFKVFETKTK DKQARLKESG
260 270 280 290 300
RELAVSWALC AVCLVGHLTH FLGVNAPWIH AIHSTGFHVS LCLITLLGPG
310 320 330 340 350
RKLVLDGIKS LLKGSPNMNT LVGLGALSSF SVSSLAAMIP KLGWKTFFEE
360 370 380 390 400
PVMLIAFVLL GRNLEQRAKI KATSDMTGLL SVLPSKARLL LDGDLQNSTV
410 420 430 440 450
EVPCNSLSVG DLVVILPGDR VPADGVVKSG RSTIDESSFT GEPLPVTKES
460 470 480 490 500
GSQVAAGSIN LNGTLTVEVH RSGGETAVGD IIRLVEEAQS REAPVQQLVD
510 520 530 540 550
KVAGRFTYGV MALSAATFTF WNLFGAHVLP SALHNGSPMS LALQLSCSVL
560 570 580 590 600
VVACPCALGL ATPTAMLVGT SLGARRGLLL RGGDILEKFS LVDTVVFDKT
610 620 630 640 650
GTLTKGHPVV TEVIIPENPR HNLNDTWSEV EVLMLAAAVE SNTTHPVGKA
660 670 680 690 700
IVKAARARNC QTMKAEDGTF TEEPGSGAVA IVNNKRVTVG TLEWVKRHGA
710 720 730 740 750
TGNSLLALEE HEINNQSVVY IGVDNTLAAV IRFEDKVRED AAQVVENLTR
760 770 780 790 800
QGIDVYMLSG DKRNAANYVA SVVGINHERV IAGVKPAEKK NFINELQKNK
810 820 830 840 850
KIVAMVGDGI NDAAALASSN VGVAMGGGAG AASEVSPVVL MGNRLTQLLD
860 870 880 890 900
AMELSRQTMK TVKQNLWWAF GYNIVGIPIA AGVLLPLTGT MLTPSMAGAL
910 920 930 940
MGVSSLGVMT NSLLLRYRFF SNRNDKNVKP EPKEGTKQPH ENTRWKQSS
Length:949
Mass (Da):99,997
Last modified:May 1, 2000 - v1
Checksum:i716F5E6E63B7F9B8
GO
Isoform 2 (identifier: Q9SZC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-237: DLVTENFFKVFET → GEVPEDIAGEFAP
     238-949: Missing.

Show »
Length:237
Mass (Da):24,013
Checksum:iC06AAE221D146D34
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti215T → S in strain: cv. Landsberg erecta. 1
Natural varianti238 – 239KT → QP in strain: cv. Landsberg erecta. 2
Natural varianti259L → P in strain: cv. Landsberg erecta. 1
Natural varianti384P → A in strain: cv. Landsberg erecta. 1
Natural varianti904S → T in strain: cv. Landsberg erecta. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040517225 – 237DLVTE…KVFET → GEVPEDIAGEFAP in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_040518238 – 949Missing in isoform 2. 1 PublicationAdd BLAST712

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89981 mRNA Translation: BAA23769.1
AL031394 Genomic DNA Translation: CAA20565.1
AL035678 Genomic DNA Translation: CAB38810.1
AL161583 Genomic DNA Translation: CAB80069.1
CP002687 Genomic DNA Translation: AEE86239.1
CP002687 Genomic DNA Translation: AEE86240.1
AY059869 mRNA Translation: AAL24351.1
AY093320 mRNA Translation: AAM13319.1
AK229051 mRNA Translation: BAF00934.1
PIRiT06003
RefSeqiNP_567924.1, NM_119506.4 [Q9SZC9-2]
NP_974675.1, NM_202946.3 [Q9SZC9-1]
UniGeneiAt.48932

Genome annotation databases

EnsemblPlantsiAT4G33520.1; AT4G33520.1; AT4G33520 [Q9SZC9-2]
AT4G33520.2; AT4G33520.2; AT4G33520 [Q9SZC9-1]
GeneIDi829490
GrameneiAT4G33520.1; AT4G33520.1; AT4G33520 [Q9SZC9-2]
AT4G33520.2; AT4G33520.2; AT4G33520 [Q9SZC9-1]
KEGGiath:AT4G33520

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHMA6_ARATH
AccessioniPrimary (citable) accession number: Q9SZC9
Secondary accession number(s): O48600
, O81870, Q0WPL5, Q93YP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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