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Protein

Alpha-mannosidase I MNS5

Gene

MNS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can convert Man9GlcNAc2 and Man8GlcNAc2 into N-glycans with a terminal alpha-1,6-linked Man residue in the C-branch. Functions in the formation of unique N-glycan structures that are specifically recognized by components of the endoplasmic reticulum-associated degradation (ERAD) machinery, which leads to the degradation of misfolded glycoproteins. Most likely generates N-glycan signal on misfolded glycoproteins that is subsequently recognized by OS9. Required for ERAD of the heavily glycosylated and misfolded BRI1 variants BRI1-5 and BRI1-9. Does not seem to play role in N-glycan processing of correctly folded proteins destined for secretion.1 Publication

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei134Proton donorBy similarity1
Active sitei274By similarity1
Active sitei367Proton donorBy similarity1
Active sitei388By similarity1
Metal bindingi471CalciumBy similarity1

GO - Molecular functioni

  • alpha-mannosidase activity Source: TAIR
  • calcium ion binding Source: InterPro
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-ATH-901032 ER Quality Control Compartment (ERQC)
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase I MNS5 (EC:3.2.1.-1 Publication)
Gene namesi
Name:MNS5
Ordered Locus Names:At1g27520
ORF Names:T17H3.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G27520
TAIRilocus:2196874 AT1G27520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini27 – 574LumenalSequence analysisAdd BLAST548

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi388E → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003979371 – 574Alpha-mannosidase I MNS5Add BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi121N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi201N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi349N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi494N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SXC9
PRIDEiQ9SXC9

Expressioni

Gene expression databases

ExpressionAtlasiQ9SXC9 baseline and differential
GenevisibleiQ9SXC9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G27520.1

Structurei

3D structure databases

ProteinModelPortaliQ9SXC9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2429 Eukaryota
ENOG410XS6F LUCA
HOGENOMiHOG000004833
InParanoidiQ9SXC9
KOiK10084
OMAiGKSVHRP
OrthoDBiEOG0936070U
PhylomeDBiQ9SXC9

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SXC9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSCPIHPRRL FLCLLISLTF FVVDPSSQHI EVKKKQMREK VREMFYHAYD
60 70 80 90 100
NYMTYAFPHD ELKPLTKSFT DSLSELGNLK LEHLPTDYNG SAVTLVESLS
110 120 130 140 150
SLAILGNSTE FEKGVLWLSE NLTFDIDARV NLFECNIRVL GGLISAHLLA
160 170 180 190 200
IDPNNRLIQG SYNNQLLRLA EDLGKRFLPA FETPTGLPYA WINLKNGVME
210 220 230 240 250
NETTETSTSG CGSLVLEMGA LSRLTGDPRF ESAALRALRQ LWRMRSSLDL
260 270 280 290 300
LGTTLDVVTG EWIEYSSSIG AGVDSFYEYL LKAYILFGKE DYWRMFHSAY
310 320 330 340 350
LASQKYFRHG PWYHEANMWS GKPTYWQLTS LQAFWPGLQV LVGDIAAANS
360 370 380 390 400
SHREFFHVWE KFGVLPERYL LDHQIIHPTM KYYPLRPELA ESTFYLYQAT
410 420 430 440 450
KDPWYLDVGE SMVKSLNLYT KVPGGFASVR DVTTMQLEDH QHSFFLAETC
460 470 480 490 500
KYLYLLFDDS FVAKRNYIFT TEGHPIQVVS SWHEKLPETY FSGNWTLSKS
510 520 530 540 550
GAWESRASAL SLQVCPLISL NSRHPEQQRE SACHVLDEQI NHKCWSNKEC
560 570
GVDATTCRLR TCSGVGYCGL WNPL
Length:574
Mass (Da):65,708
Last modified:May 1, 2000 - v1
Checksum:i4ACC456DE487EA93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005916 Genomic DNA Translation: AAD45990.1
CP002684 Genomic DNA Translation: AEE30841.1
AY056382 mRNA Translation: AAL08238.1
PIRiC86400
RefSeqiNP_564288.1, NM_102516.4
UniGeneiAt.24198

Genome annotation databases

EnsemblPlantsiAT1G27520.1; AT1G27520.1; AT1G27520
GeneIDi839643
GrameneiAT1G27520.1; AT1G27520.1; AT1G27520
KEGGiath:AT1G27520

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005916 Genomic DNA Translation: AAD45990.1
CP002684 Genomic DNA Translation: AEE30841.1
AY056382 mRNA Translation: AAL08238.1
PIRiC86400
RefSeqiNP_564288.1, NM_102516.4
UniGeneiAt.24198

3D structure databases

ProteinModelPortaliQ9SXC9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G27520.1

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Proteomic databases

PaxDbiQ9SXC9
PRIDEiQ9SXC9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G27520.1; AT1G27520.1; AT1G27520
GeneIDi839643
GrameneiAT1G27520.1; AT1G27520.1; AT1G27520
KEGGiath:AT1G27520

Organism-specific databases

AraportiAT1G27520
TAIRilocus:2196874 AT1G27520

Phylogenomic databases

eggNOGiKOG2429 Eukaryota
ENOG410XS6F LUCA
HOGENOMiHOG000004833
InParanoidiQ9SXC9
KOiK10084
OMAiGKSVHRP
OrthoDBiEOG0936070U
PhylomeDBiQ9SXC9

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-ATH-901032 ER Quality Control Compartment (ERQC)

Miscellaneous databases

PROiPR:Q9SXC9

Gene expression databases

ExpressionAtlasiQ9SXC9 baseline and differential
GenevisibleiQ9SXC9 AT

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMNS5_ARATH
AccessioniPrimary (citable) accession number: Q9SXC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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