UniProtKB - Q9SV68 (QORH_ARATH)
Chloroplast envelope quinone oxidoreductase homolog
CEQORH
Functioni
NADPH-dependent alpha,beta-unsaturated oxoene reductase reducing the double bond of medium-chain (C9) to long-chain (C18) reactive electrophile species deriving from poly-unsaturated fatty acid peroxides. The best substrates are 13-lipoxygenase-derived gamma-ketols, but is unable to reduce the double bond of short-chain alkenals and alkenones such as acrolein, crotonaldehyde, 3-buten-2-one, 4-hexen-3-one and trans-2-hexenal, or quinones such as duroquinone, decylubiquinone, coenzyme Q0, menadione, menaquinone and phylloquinone. Can use trans-2-nonenal, trans-3-decen-2-one, 4-hydroxynonenal, 12-oxo-10(E) dodecanoate (traumatin), 4-oxononenal, trans-1,3 diphenyl-2-propenone, trans-1,4-diphenyl-2-butene-1,4-dione, 9-oxo-12,13-epoxy-(10E)-octadecenoic acid (trans-EKODE-1b), 9-hydroxy-12-oxo-10(E)-octadecenoic acid, 9-Hydroxy-12-oxo-10(E),15(Z)-octadecadienoic acid and 9,13-dihydroxy-10-oxo-11-octadecenoic acid as substrates, but has no activity with 13(R,S)-hydroperoxy-9(Z),11(E)-octadecadienoic acid (13-HPOD), 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid, 13-hydroxy-12-oxo-9(Z)-octadecenoic acid, 9-oxo-10(E),12(Z)-octadecadienoic acid (9-KODE), 13-oxo-9(Z),11(E)-octadecadienoic acid (13-KODE) and 12-oxo-10,15(Z)-phytodienoic acid (12-OPDA).
1 PublicationMiscellaneous
Kineticsi
- KM=200 µM for 4-oxo-nonenal1 Publication
- KM=9 µM for trans-1,4-diphenyl-2-butene-1,4-dione1 Publication
- KM=14 µM for 9-hydroxy-12-oxo-10(E)-octadecenoic acid1 Publication
- KM=10 µM for 9-Hydroxy-12-oxo-10(E),15(Z)-octadecadienoic acid1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 58 | SubstrateCombined sources1 Publication | 1 |
GO - Molecular functioni
- oxidoreductase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Names & Taxonomyi
Protein namesi | Recommended name: Chloroplast envelope quinone oxidoreductase homolog1 Publication (EC:1.3.1.-1 Publication)Short name: ceQORH1 Publication |
Gene namesi | Name:CEQORH1 Publication Ordered Locus Names:At4g13010Imported ORF Names:F25G13_100Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G13010 |
TAIRi | locus:2123206, AT4G13010 |
Subcellular locationi
Chloroplast
- chloroplast inner membrane 3 Publications
Note: Faces the stroma side of the membrane (PubMed:20424175). Targeting to the chloroplast inner membrane is mediated by HP30-1, HP30-2 and HP20 (PubMed:24248378).2 Publications
Chloroplast
- chloroplast Source: TAIR
- chloroplast envelope Source: TAIR
- chloroplast inner membrane Source: UniProtKB-SubCell
- chloroplast thylakoid membrane Source: TAIR
Cytosol
- cytosol Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Keywords - Cellular componenti
Chloroplast, Membrane, Plastid, Plastid inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000160915 | 1 – 329 | Chloroplast envelope quinone oxidoreductase homologAdd BLAST | 329 |
Proteomic databases
PaxDbi | Q9SV68 |
PRIDEi | Q9SV68 |
ProteomicsDBi | 236602 |
Expressioni
Inductioni
Gene expression databases
ExpressionAtlasi | Q9SV68, baseline and differential |
Genevisiblei | Q9SV68, AT |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9SV68 |
SMRi | Q9SV68 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1198, Eukaryota |
HOGENOMi | CLU_026673_3_3_1 |
InParanoidi | Q9SV68 |
OMAi | MKAWLYS |
OrthoDBi | 727365at2759 |
PhylomeDBi | Q9SV68 |
Family and domain databases
InterProi | View protein in InterPro IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAGKLMHALQ YNSYGGGAAG LEHVQVPVPT PKSNEVCLKL EATSLNPVDW
60 70 80 90 100
KIQKGMIRPF LPRKFPCIPA TDVAGEVVEV GSGVKNFKAG DKVVAVLSHL
110 120 130 140 150
GGGGLAEFAV ATEKLTVKRP QEVGAAEAAA LPVAGLTALQ ALTNPAGLKL
160 170 180 190 200
DGTGKKANIL VTAASGGVGH YAVQLAKLAN AHVTATCGAR NIEFVKSLGA
210 220 230 240 250
DEVLDYKTPE GAALKSPSGK KYDAVVHCAN GIPFSVFEPN LSENGKVIDI
260 270 280 290 300
TPGPNAMWTY AVKKITMSKK QLVPLLLIPK AENLEFMVNL VKEGKVKTVI
310 320
DSKHPLSKAE DAWAKSIDGH ATGKIIVEP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL079349 Genomic DNA Translation: CAB45500.1 AL161535 Genomic DNA Translation: CAB78343.1 CP002687 Genomic DNA Translation: AEE83213.1 AY045857 mRNA Translation: AAK76531.1 AF412068 mRNA Translation: AAL06521.1 AY117154 mRNA Translation: AAM51229.1 AY056396 mRNA Translation: AAL08252.1 |
PIRi | T10203 |
RefSeqi | NP_193037.1, NM_117370.3 |
Genome annotation databases
EnsemblPlantsi | AT4G13010.1; AT4G13010.1; AT4G13010 |
GeneIDi | 826914 |
Gramenei | AT4G13010.1; AT4G13010.1; AT4G13010 |
KEGGi | ath:AT4G13010 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL079349 Genomic DNA Translation: CAB45500.1 AL161535 Genomic DNA Translation: CAB78343.1 CP002687 Genomic DNA Translation: AEE83213.1 AY045857 mRNA Translation: AAK76531.1 AF412068 mRNA Translation: AAL06521.1 AY117154 mRNA Translation: AAM51229.1 AY056396 mRNA Translation: AAL08252.1 |
PIRi | T10203 |
RefSeqi | NP_193037.1, NM_117370.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5A3J | X-ray | 2.78 | A/B/C/D/E/F/G/H/I/J/K/L | 1-329 | [»] | |
5A3V | X-ray | 2.34 | A/B | 1-329 | [»] | |
5A4D | X-ray | 2.81 | A/B/C/D/E/F/G/H | 1-329 | [»] | |
AlphaFoldDBi | Q9SV68 | |||||
SMRi | Q9SV68 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT4G13010.1 |
Proteomic databases
PaxDbi | Q9SV68 |
PRIDEi | Q9SV68 |
ProteomicsDBi | 236602 |
Genome annotation databases
EnsemblPlantsi | AT4G13010.1; AT4G13010.1; AT4G13010 |
GeneIDi | 826914 |
Gramenei | AT4G13010.1; AT4G13010.1; AT4G13010 |
KEGGi | ath:AT4G13010 |
Organism-specific databases
Araporti | AT4G13010 |
TAIRi | locus:2123206, AT4G13010 |
Phylogenomic databases
eggNOGi | KOG1198, Eukaryota |
HOGENOMi | CLU_026673_3_3_1 |
InParanoidi | Q9SV68 |
OMAi | MKAWLYS |
OrthoDBi | 727365at2759 |
PhylomeDBi | Q9SV68 |
Miscellaneous databases
PROi | PR:Q9SV68 |
Gene expression databases
ExpressionAtlasi | Q9SV68, baseline and differential |
Genevisiblei | Q9SV68, AT |
Family and domain databases
InterProi | View protein in InterPro IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | QORH_ARATH | |
Accessioni | Q9SV68Primary (citable) accession number: Q9SV68 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families