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UniProtKB - Q9SUC5 (FAH2_ARATH)
Protein
Dihydroceramide fatty acyl 2-hydroxylase FAH2
Gene
FAH2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Fatty acid 2-hydroxylase involved in the alpha-hydroxylation of the long-chain fatty acid (LCFA) palmitic acid. Probably involved in the resistance response to oxidative stress.
2 PublicationsCatalytic activityi
- an N-(1,2-saturated acyl)sphinganine + 2 Fe(II)-[cytochrome b5] + 2 H+ + O2 = an N-[(2'R)-hydroxyacyl]sphinganine + 2 Fe(III)-[cytochrome b5] + H2O1 PublicationEC:1.14.18.71 Publication
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit that likely form a catalytic dimetal center.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 102 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 107 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 123 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 126 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 127 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 181 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 185 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 201 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 204 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 205 | Zinc 2; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- fatty acid alpha-hydroxylase activity Source: GO_Central
- iron ion binding Source: InterPro
- sphingolipid very long chain fatty acid alpha-hydroxylase activity Source: RHEA
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-KW
- fatty acid metabolic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | ARA:AT4G20870-MONOMER |
BRENDAi | 1.14.18.7, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydroceramide fatty acyl 2-hydroxylase FAH2Curated (EC:1.14.18.71 Publication)Alternative name(s): Fatty acid 2-hydroxylase 21 Publication Short name: AtFAH21 Publication |
Gene namesi | Name:FAH21 Publication Ordered Locus Names:At4g20870Imported ORF Names:T13K14.30Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G20870 |
TAIRi | locus:2133054, AT4G20870 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 54 – 74 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 77 – 97 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 134 – 154 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 156 – 176 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype under normal growth conditions.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000419657 | 1 – 237 | Dihydroceramide fatty acyl 2-hydroxylase FAH2Add BLAST | 237 |
Proteomic databases
PaxDbi | Q9SUC5 |
ProteomicsDBi | 230955 |
Expressioni
Tissue specificityi
Expressed in leaves, roots, flowers and seeds.1 Publication
Inductioni
Not induced by H2O2 treatment.1 Publication
Gene expression databases
ExpressionAtlasi | Q9SUC5, baseline and differential |
Genevisiblei | Q9SUC5, AT |
Interactioni
Subunit structurei
Interacts with CYTB5-A, CYTB5-B, CYTB5-C and CYTB5-D.
1 PublicationProtein-protein interaction databases
BioGRIDi | 13126, 1 interactor |
IntActi | Q9SUC5, 1 interactor |
STRINGi | 3702.AT4G20870.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the sterol desaturase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0539, Eukaryota |
HOGENOMi | CLU_034756_1_0_1 |
InParanoidi | Q9SUC5 |
OMAi | IQEVALM |
OrthoDBi | 1049908at2759 |
PhylomeDBi | Q9SUC5 |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase IPR014430, Scs7 |
PANTHERi | PTHR12863, PTHR12863, 1 hit |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SUC5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVAERYTVDL NKPLVFQVGH LGEEYQEWIH QPIVCVEGPR FFESDFWEFL
60 70 80 90 100
TRTVWWAIPT IWLPVVCYVL SISASKGLTF PQIGLIVAFG VLTWTLLEYT
110 120 130 140 150
LHRFLFHIQT KSYWANTAHY LLHGCHHKHP QDGLRLVFPP TATAILLVPL
160 170 180 190 200
WKLLHLLATP ATAPAILGGI LFGYVMYDIT HYYLHHGQPK EPTFKHLKKY
210 220 230
HLNHHFRIQD KGYGITSSLW DKVFGTLPGI KAAAKKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL080282 Genomic DNA Translation: CAB45882.1 AL161553 Genomic DNA Translation: CAB79087.1 CP002687 Genomic DNA Translation: AEE84370.1 CP002687 Genomic DNA Translation: ANM67123.1 AY044315 mRNA Translation: AAK73256.1 AY058151 mRNA Translation: AAL25567.1 AY101538 mRNA Translation: AAM26659.1 |
PIRi | T10629 |
RefSeqi | NP_001328972.1, NM_001341459.1 NP_193819.1, NM_118205.2 |
Genome annotation databases
EnsemblPlantsi | AT4G20870.1; AT4G20870.1; AT4G20870 AT4G20870.2; AT4G20870.2; AT4G20870 |
GeneIDi | 827835 |
Gramenei | AT4G20870.1; AT4G20870.1; AT4G20870 AT4G20870.2; AT4G20870.2; AT4G20870 |
KEGGi | ath:AT4G20870 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL080282 Genomic DNA Translation: CAB45882.1 AL161553 Genomic DNA Translation: CAB79087.1 CP002687 Genomic DNA Translation: AEE84370.1 CP002687 Genomic DNA Translation: ANM67123.1 AY044315 mRNA Translation: AAK73256.1 AY058151 mRNA Translation: AAL25567.1 AY101538 mRNA Translation: AAM26659.1 |
PIRi | T10629 |
RefSeqi | NP_001328972.1, NM_001341459.1 NP_193819.1, NM_118205.2 |
3D structure databases
SMRi | Q9SUC5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 13126, 1 interactor |
IntActi | Q9SUC5, 1 interactor |
STRINGi | 3702.AT4G20870.1 |
Proteomic databases
PaxDbi | Q9SUC5 |
ProteomicsDBi | 230955 |
Protocols and materials databases
DNASUi | 827835 |
Genome annotation databases
EnsemblPlantsi | AT4G20870.1; AT4G20870.1; AT4G20870 AT4G20870.2; AT4G20870.2; AT4G20870 |
GeneIDi | 827835 |
Gramenei | AT4G20870.1; AT4G20870.1; AT4G20870 AT4G20870.2; AT4G20870.2; AT4G20870 |
KEGGi | ath:AT4G20870 |
Organism-specific databases
Araporti | AT4G20870 |
TAIRi | locus:2133054, AT4G20870 |
Phylogenomic databases
eggNOGi | KOG0539, Eukaryota |
HOGENOMi | CLU_034756_1_0_1 |
InParanoidi | Q9SUC5 |
OMAi | IQEVALM |
OrthoDBi | 1049908at2759 |
PhylomeDBi | Q9SUC5 |
Enzyme and pathway databases
BioCyci | ARA:AT4G20870-MONOMER |
BRENDAi | 1.14.18.7, 399 |
Miscellaneous databases
PROi | PR:Q9SUC5 |
Gene expression databases
ExpressionAtlasi | Q9SUC5, baseline and differential |
Genevisiblei | Q9SUC5, AT |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase IPR014430, Scs7 |
PANTHERi | PTHR12863, PTHR12863, 1 hit |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FAH2_ARATH | |
Accessioni | Q9SUC5Primary (citable) accession number: Q9SUC5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families