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Protein

Ethylene-responsive transcription factor RAP2-12

Gene

RAP2-12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor involved in the activation of hypoxic gene expression and in ethylene response. Partially redundant with RAP2-2. Acts as a downstream regulator in the ethylene signaling pathway.2 Publications

Miscellaneous

The N-terminus qualifies RAP2-12 as candidate substrate of the N-end rule pathway for protein destabilization. The oxygen-dependent oxidation of Cys-2 prevents hypoxic gene expression via the destabilization of RAP2-12 in air. When the oxygen concentration decreases, Cys oxidation is prevented and an active RAP2.12 accumulates in the nucleus.

Activity regulationi

Ethylene-responsive transcription factor RAP2-12, N-terminally processed: The N-terminal cysteine residue of can be oxidized by PCO1 or PCO2, thus preparing the protein for N-end rule pathway-mediated proteasomal degradation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi124 – 181AP2/ERFPROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

  • DNA-binding transcription factor activity Source: TAIR
  • sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • detection of hypoxia Source: TAIR
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • regulation of root development Source: TAIR
  • response to hypoxia Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processEthylene signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor RAP2-12
Alternative name(s):
Protein RELATED TO APETALA2 12
Gene namesi
Name:RAP2-12
Synonyms:ERF074
Ordered Locus Names:At1g53910
ORF Names:T18A20.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G53910
TAIRilocus:2197076 AT1G53910

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2C → A: Increased lifetime of the protein and accumulation in the nucleus. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002979411 – 358Ethylene-responsive transcription factor RAP2-12Add BLAST358

Proteomic databases

PaxDbiQ9SSA8

Expressioni

Tissue specificityi

Highly expressed in seedlings, leaves, flowers, siliques and germinating seeds.1 Publication

Inductioni

Up-regulated by hypoxia but not by ethylene.1 Publication

Gene expression databases

ExpressionAtlasiQ9SSA8 baseline and differential
GenevisibleiQ9SSA8 AT

Interactioni

Subunit structurei

Interacts with ACBP1 and ACBP2.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi27054, 2 interactors
DIPiDIP-60469N
ELMiQ9SSA8
IntActiQ9SSA8, 10 interactors
STRINGi3702.AT1G53910.1

Structurei

3D structure databases

ProteinModelPortaliQ9SSA8
SMRiQ9SSA8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminus (1-13) is important for the regulation of the oxygen-dependent activation of the transcription factor activity, an internal region (123-177) is required for the interactions with ACPB1 and ACPB2, while the C-terminus (343-358) is required for positive regulation of gene transcription.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IWFM Eukaryota
ENOG410YERP LUCA
HOGENOMiHOG000237624
InParanoidiQ9SSA8
KOiK09286
OMAiWSIDEIH
OrthoDBiEOG09360HNM
PhylomeDBiQ9SSA8

Family and domain databases

CDDicd00018 AP2, 1 hit
Gene3Di3.30.730.10, 1 hit
InterProiView protein in InterPro
IPR001471 AP2/ERF_dom
IPR036955 AP2/ERF_dom_sf
IPR016177 DNA-bd_dom_sf
PfamiView protein in Pfam
PF00847 AP2, 1 hit
PRINTSiPR00367 ETHRSPELEMNT
SMARTiView protein in SMART
SM00380 AP2, 1 hit
SUPFAMiSSF54171 SSF54171, 1 hit
PROSITEiView protein in PROSITE
PS51032 AP2_ERF, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
Note: A number of isoforms are produced. According to EST sequences.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: Q9SSA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCGGAIISDF IPPPRSRRVT SEFIWPDLKK NLKGSKKSSK NRSNFFDFDA
60 70 80 90 100
EFEADFQGFK DDSSIDCDDD FDVGDVFADV KPFVFTSTPK PAVSAAAEGS
110 120 130 140 150
VFGKKVTGLD GDAEKSANRK RKNQYRGIRQ RPWGKWAAEI RDPREGARIW
160 170 180 190 200
LGTFKTAEEA ARAYDAAARR IRGSKAKVNF PEENMKANSQ KRSVKANLQK
210 220 230 240 250
PVAKPNPNPS PALVQNSNIS FENMCFMEEK HQVSNNNNNQ FGMTNSVDAG
260 270 280 290 300
CNGYQYFSSD QGSNSFDCSE FGWSDQAPIT PDISSAVINN NNSALFFEEA
310 320 330 340 350
NPAKKLKSMD FETPYNNTEW DASLDFLNED AVTTQDNGAN PMDLWSIDEI

HSMIGGVF
Length:358
Mass (Da):39,801
Last modified:May 1, 2000 - v1
Checksum:iCF8232CBE3C9FE35
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A8MRC0A8MRC0_ARATH
Related to AP2 12
RAP2.12 related to AP2 12, At1g53910, T18A20.14, T18A20_14
356Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112D → E in AAM65746 (Ref. 4) Curated1
Sequence conflicti185M → L in AAM65746 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009324 Genomic DNA Translation: AAF02863.1
CP002684 Genomic DNA Translation: AEE33022.1
CP002684 Genomic DNA Translation: AEE33023.1
AY037260 mRNA Translation: AAK59861.1
AY057545 mRNA Translation: AAL09785.1
AY113051 mRNA Translation: AAM47359.1
AY088204 mRNA Translation: AAM65746.1
AF003105 mRNA Translation: AAC49778.1
PIRiD96579
RefSeqiNP_001031185.1, NM_001036108.3 [Q9SSA8-1]
NP_175794.1, NM_104269.4 [Q9SSA8-1]
UniGeneiAt.20952
At.21295

Genome annotation databases

EnsemblPlantsiAT1G53910.1; AT1G53910.1; AT1G53910 [Q9SSA8-1]
AT1G53910.2; AT1G53910.2; AT1G53910 [Q9SSA8-1]
GeneIDi841829
GrameneiAT1G53910.1; AT1G53910.1; AT1G53910 [Q9SSA8-1]
AT1G53910.2; AT1G53910.2; AT1G53910 [Q9SSA8-1]
KEGGiath:AT1G53910

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRA212_ARATH
AccessioniPrimary (citable) accession number: Q9SSA8
Secondary accession number(s): O23113, Q8L9V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: May 1, 2000
Last modified: September 12, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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