UniProtKB - Q9SRZ6 (ICDHC_ARATH)
Protein
Cytosolic isocitrate dehydrogenase [NADP]
Gene
CICDH
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
May supply 2-oxoglutarate for amino acid biosynthesis and ammonia assimilation via the glutamine synthetase/glutamate synthase (GS/GOGAT) pathway. May be involved in the production of NADPH to promote redox signaling or homeostasis in response to oxidative stress.1 Publication
Catalytic activityi
- EC:1.1.1.42
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 79 | SubstrateBy similarity | 1 | |
Binding sitei | 84 | NADPBy similarity | 1 | |
Binding sitei | 111 | SubstrateBy similarity | 1 | |
Binding sitei | 134 | SubstrateBy similarity | 1 | |
Sitei | 141 | Critical for catalysisBy similarity | 1 | |
Sitei | 212 | Critical for catalysisBy similarity | 1 | |
Metal bindingi | 252 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 260 | NADPBy similarity | 1 | |
Metal bindingi | 275 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 328 | NADP; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 77 – 79 | NADPBy similarity | 3 | |
Nucleotide bindingi | 310 – 315 | NADPBy similarity | 6 |
GO - Molecular functioni
- copper ion binding Source: TAIR
- isocitrate dehydrogenase (NADP+) activity Source: TAIR
- magnesium ion binding Source: InterPro
- NAD binding Source: InterPro
GO - Biological processi
- defense response to bacterium Source: TAIR
- isocitrate metabolic process Source: TAIR
- NADP metabolic process Source: TAIR
- response to cadmium ion Source: TAIR
- response to zinc ion Source: TAIR
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Plant defense, Stress response, Tricarboxylic acid cycle |
Ligand | Magnesium, Manganese, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:AT1G65930-MONOMER |
BRENDAi | 1.1.1.42, 399 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CICDH Ordered Locus Names:At1g65930 ORF Names:F12P19.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G65930 |
TAIRi | locus:2009759, AT1G65930 |
Subcellular locationi
Cytosol
Chloroplast
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: TAIR
Extracellular region or secreted
- apoplast Source: TAIR
Mitochondrion
- mitochondrion Source: GO_Central
Nucleus
- nucleus Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- plasmodesma Source: TAIR
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Slight reduction in plant growth. Constitutive expression of pathogenesis-related genes and enhanced resistance to the bacterial pathogen P.syringae pv. tomato.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000421963 | 1 – 410 | Cytosolic isocitrate dehydrogenase [NADP]Add BLAST | 410 |
Proteomic databases
PaxDbi | Q9SRZ6 |
PRIDEi | Q9SRZ6 |
ProteomicsDBi | 228756 |
PTM databases
iPTMneti | Q9SRZ6 |
MetOSitei | Q9SRZ6 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9SRZ6, baseline and differential |
Genevisiblei | Q9SRZ6, AT |
Interactioni
Binary interactionsi
Q9SRZ6
With | #Exp. | IntAct |
---|---|---|
TRX3 [Q42403] | 2 | EBI-449319,EBI-449157 |
Protein-protein interaction databases
BioGRIDi | 28126, 2 interactors |
IntActi | Q9SRZ6, 2 interactors |
STRINGi | 3702.AT1G65930.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 96 – 102 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Belongs to the isocitrate and isopropylmalate dehydrogenases family.Curated
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | Q9SRZ6 |
OMAi | NYATKRI |
OrthoDBi | 769322at2759 |
PhylomeDBi | Q9SRZ6 |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SRZ6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAFEKIKVAN PIVEMDGDEM TRVIWKSIKD KLITPFVELD IKYFDLGLPH
60 70 80 90 100
RDATDDKVTI ESAEATKKYN VAIKCATITP DEGRVTEFGL KQMWRSPNGT
110 120 130 140 150
IRNILNGTVF REPIICKNVP KLVPGWTKPI CIGRHAFGDQ YRATDAVIKG
160 170 180 190 200
PGKLTMTFEG KDGKTETEVF TFTGEGGVAM AMYNTDESIR AFADASMNTA
210 220 230 240 250
YEKKWPLYLS TKNTILKKYD GRFKDIFQEV YEASWKSKYD AAGIWYEHRL
260 270 280 290 300
IDDMVAYALK SEGGYVWACK NYDGDVQSDF LAQGFGSLGL MTSVLVCPDG
310 320 330 340 350
KTIEAEAAHG TVTRHFRVHQ KGGETSTNSI ASIFAWTRGL AHRAKLDDNA
360 370 380 390 400
KLLDFTEKLE AACVGTVESG KMTKDLALII HGSKLSRDTY LNTEEFIDAV
410
AAELKERLNA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 181 | A → V in CAD24782 (Ref. 1) Curated | 1 | |
Sequence conflicti | 262 | E → K in AAM65674 (Ref. 5) Curated | 1 | |
Sequence conflicti | 280 | F → L in CAD24782 (Ref. 1) Curated | 1 | |
Sequence conflicti | 302 | T → S in CAD24782 (Ref. 1) Curated | 1 | |
Sequence conflicti | 322 | G → A in AAM65674 (Ref. 5) Curated | 1 | |
Sequence conflicti | 395 | E → D in AAM13090 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ437268 mRNA Translation: CAD24782.1 AC009513 Genomic DNA Translation: AAF06054.1 CP002684 Genomic DNA Translation: AEE34442.1 AF419575 mRNA Translation: AAL31907.1 AY045631 mRNA Translation: AAK73989.1 AY093091 mRNA Translation: AAM13090.1 AY097340 mRNA Translation: AAM19856.1 BT002400 mRNA Translation: AAO00760.1 AY088129 mRNA Translation: AAM65674.1 |
PIRi | F96683 |
RefSeqi | NP_176768.1, NM_105265.5 |
Genome annotation databases
EnsemblPlantsi | AT1G65930.1; AT1G65930.1; AT1G65930 |
GeneIDi | 842905 |
Gramenei | AT1G65930.1; AT1G65930.1; AT1G65930 |
KEGGi | ath:AT1G65930 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ437268 mRNA Translation: CAD24782.1 AC009513 Genomic DNA Translation: AAF06054.1 CP002684 Genomic DNA Translation: AEE34442.1 AF419575 mRNA Translation: AAL31907.1 AY045631 mRNA Translation: AAK73989.1 AY093091 mRNA Translation: AAM13090.1 AY097340 mRNA Translation: AAM19856.1 BT002400 mRNA Translation: AAO00760.1 AY088129 mRNA Translation: AAM65674.1 |
PIRi | F96683 |
RefSeqi | NP_176768.1, NM_105265.5 |
3D structure databases
SMRi | Q9SRZ6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 28126, 2 interactors |
IntActi | Q9SRZ6, 2 interactors |
STRINGi | 3702.AT1G65930.1 |
PTM databases
iPTMneti | Q9SRZ6 |
MetOSitei | Q9SRZ6 |
Proteomic databases
PaxDbi | Q9SRZ6 |
PRIDEi | Q9SRZ6 |
ProteomicsDBi | 228756 |
Genome annotation databases
EnsemblPlantsi | AT1G65930.1; AT1G65930.1; AT1G65930 |
GeneIDi | 842905 |
Gramenei | AT1G65930.1; AT1G65930.1; AT1G65930 |
KEGGi | ath:AT1G65930 |
Organism-specific databases
Araporti | AT1G65930 |
TAIRi | locus:2009759, AT1G65930 |
Phylogenomic databases
eggNOGi | KOG1526, Eukaryota |
HOGENOMi | CLU_023296_1_1_1 |
InParanoidi | Q9SRZ6 |
OMAi | NYATKRI |
OrthoDBi | 769322at2759 |
PhylomeDBi | Q9SRZ6 |
Enzyme and pathway databases
BioCyci | MetaCyc:AT1G65930-MONOMER |
BRENDAi | 1.1.1.42, 399 |
Miscellaneous databases
PROi | PR:Q9SRZ6 |
Gene expression databases
ExpressionAtlasi | Q9SRZ6, baseline and differential |
Genevisiblei | Q9SRZ6, AT |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR004790, Isocitrate_DH_NADP IPR024084, IsoPropMal-DH-like_dom |
PANTHERi | PTHR11822, PTHR11822, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
PIRSFi | PIRSF000108, IDH_NADP, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00127, nadp_idh_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ICDHC_ARATH | |
Accessioni | Q9SRZ6Primary (citable) accession number: Q9SRZ6 Secondary accession number(s): Q8L9Z4, Q8RWH2, Q8RYD5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2013 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families