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Protein

U-box domain-containing protein 9

Gene

PUB9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as an E3 ubiquitin ligase (By similarity). May be involved in the abscisic acid-mediated signaling pathway, at least during germination.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • transmembrane receptor protein serine/threonine kinase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • cellular response to abscisic acid stimulus Source: UniProtKB
  • protein ubiquitination Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processAbscisic acid signaling pathway, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
U-box domain-containing protein 9 (EC:2.3.2.27)
Alternative name(s):
Plant U-box protein 9
RING-type E3 ubiquitin transferase PUB9Curated
Gene namesi
Name:PUB9
Ordered Locus Names:At3g07360
ORF Names:F21O3.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G07360
TAIRilocus:2079706 AT3G07360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced abscisic acid (ABA) sensitivity during seed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003221541 – 460U-box domain-containing protein 9Add BLAST460

Post-translational modificationi

Phosphorylated by SD1-6 and SD1-7.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9SRT0

PTM databases

iPTMnetiQ9SRT0

Expressioni

Gene expression databases

ExpressionAtlasiQ9SRT0 baseline and differential
GenevisibleiQ9SRT0 AT

Interactioni

Subunit structurei

Binds to SD11, SD16, SD17, SD18, SD113, SD129 and SD25.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi5259, 3 interactors
STRINGi3702.AT3G07360.1

Structurei

3D structure databases

ProteinModelPortaliQ9SRT0
SMRiQ9SRT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 147U-boxAdd BLAST75
Repeati201 – 244ARM 1Add BLAST44
Repeati248 – 287ARM 2Add BLAST40
Repeati289 – 328ARM 3Add BLAST40

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0167 Eukaryota
ENOG410XRTN LUCA
HOGENOMiHOG000238412
InParanoidiQ9SRT0
OMAiQCKRNKE
OrthoDBiEOG09360A1W
PhylomeDBiQ9SRT0

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003613 Ubox_domain
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF04564 U-box, 1 hit
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
Note: A number of isoforms are produced. According to EST sequences.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9SRT0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAKTGVFDSD PTAIAKAKEL KREMKKLLIK IDDEDDLGVQ TIDQLQDALS
60 70 80 90 100
ALREATMRKM AKSSSLEMLE TVSCPEEFRC PLSNELMRDP VVLASGQTYD
110 120 130 140 150
KLFIQKWLSS GNRTCPKTQQ VLPHTALTPN LLIREMISKW CKKNGLETKS
160 170 180 190 200
QYHPNLVNED ETVTRSDREI FNSLLCKVSS SNLQDQKSAA KELRLLTRKG
210 220 230 240 250
TEFRALFGES PDEITRLVNP LLHGSNPDEK LQEDVVTTLL NISIHDDSNK
260 270 280 290 300
KLVCENPNVI PLLIDALRRG TVATRSNAAA AIFTLSALDS NKVLIGKSGI
310 320 330 340 350
LKPLIDLLEE GNPLAIKDVA AAIFTLCIAH ENRSRAVRDG AVRVLGKKIS
360 370 380 390 400
NGLYVDELLA ILAMLVTHWK AVEELGELGG VSWLLKITRE SECKRNKENA
410 420 430 440 450
IVILHTICFS DRTKWKEIKE EENAHGTITK LSREGTSRAQ RKANGILDRL
460
RKAMNLTHTA
Length:460
Mass (Da):51,279
Last modified:May 1, 2000 - v1
Checksum:iFE35CC691CE75450
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3EBB0Q3EBB0_ARATH
Plant U-box 9
PUB9 ATPUB9, plant U-box 9, At3g07360
325Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009853 Genomic DNA Translation: AAF02146.1
CP002686 Genomic DNA Translation: AEE74532.1
AY075636 mRNA Translation: AAL91644.1
RefSeqiNP_566304.1, NM_111615.4 [Q9SRT0-1]
UniGeneiAt.21635

Genome annotation databases

EnsemblPlantsiAT3G07360.1; AT3G07360.1; AT3G07360 [Q9SRT0-1]
GeneIDi819924
GrameneiAT3G07360.1; AT3G07360.1; AT3G07360 [Q9SRT0-1]
KEGGiath:AT3G07360

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009853 Genomic DNA Translation: AAF02146.1
CP002686 Genomic DNA Translation: AEE74532.1
AY075636 mRNA Translation: AAL91644.1
RefSeqiNP_566304.1, NM_111615.4 [Q9SRT0-1]
UniGeneiAt.21635

3D structure databases

ProteinModelPortaliQ9SRT0
SMRiQ9SRT0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5259, 3 interactors
STRINGi3702.AT3G07360.1

PTM databases

iPTMnetiQ9SRT0

Proteomic databases

PaxDbiQ9SRT0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G07360.1; AT3G07360.1; AT3G07360 [Q9SRT0-1]
GeneIDi819924
GrameneiAT3G07360.1; AT3G07360.1; AT3G07360 [Q9SRT0-1]
KEGGiath:AT3G07360

Organism-specific databases

AraportiAT3G07360
TAIRilocus:2079706 AT3G07360

Phylogenomic databases

eggNOGiKOG0167 Eukaryota
ENOG410XRTN LUCA
HOGENOMiHOG000238412
InParanoidiQ9SRT0
OMAiQCKRNKE
OrthoDBiEOG09360A1W
PhylomeDBiQ9SRT0

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:Q9SRT0

Gene expression databases

ExpressionAtlasiQ9SRT0 baseline and differential
GenevisibleiQ9SRT0 AT

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003613 Ubox_domain
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF04564 U-box, 1 hit
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPUB9_ARATH
AccessioniPrimary (citable) accession number: Q9SRT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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