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Protein

(Z)-3-hexen-1-ol acetyltransferase

Gene

CHAT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyltransferase involved in the production of green leaf volatiles (GLVs). Uses acetyl-CoA as substrate, but not malonyl-CoA or benzoyl-CoA. Prefers primary, medium-chain-length, aliphatic alcohols.1 Publication

Catalytic activityi

Acetyl-CoA + (3Z)-hex-3-en-ol = (3Z)-hex-3-en-1-yl acetate + CoA.2 Publications

Enzyme regulationi

Inhibited by magnesium, calcium, cobalt, zinc and copper.1 Publication

Kineticsi

  1. KM=10.5 µM for acetyl-CoA2 Publications
  2. KM=165 µM for (3Z)-hex-3-en-ol2 Publications
  3. KM=174 µM for 1-octanol2 Publications

    pH dependencei

    Optimum pH is 7.1-7.3.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei174Proton acceptorSequence analysis1
    Active sitei389Proton acceptorSequence analysis1

    GO - Molecular functioni

    GO - Biological processi

    • green leaf volatile biosynthetic process Source: TAIR

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciARA:AT3G03480-MONOMER
    MetaCyc:AT3G03480-MONOMER
    BRENDAi2.3.1.195 399

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    (Z)-3-hexen-1-ol acetyltransferase (EC:2.3.1.195)
    Gene namesi
    Name:CHAT
    Ordered Locus Names:At3g03480
    ORF Names:T21P5.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G03480
    TAIRilocus:2099704 AT3G03480

    Pathology & Biotechi

    Disruption phenotypei

    Loss of (3Z)-hex-3-en-1-yl acetate production.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004095891 – 454(Z)-3-hexen-1-ol acetyltransferaseAdd BLAST454

    Proteomic databases

    PaxDbiQ9SRQ2

    Expressioni

    Tissue specificityi

    Expressed in leaves and stems. Lower levels in flowers and barely detected in roots and siliques.1 Publication

    Inductioni

    Up-regulated by wounding with a peak 3 hours after wounding.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9SRQ2 baseline and differential
    GenevisibleiQ9SRQ2 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G03480.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SRQ2
    SMRiQ9SRQ2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the plant acyltransferase family.Curated

    Phylogenomic databases

    eggNOGiENOG410IJ7A Eukaryota
    ENOG410YDJK LUCA
    HOGENOMiHOG000237410
    InParanoidiQ9SRQ2
    KOiK18858
    OMAiNRKGVSG
    OrthoDBiEOG09360B43
    PhylomeDBiQ9SRQ2

    Family and domain databases

    Gene3Di3.30.559.10, 2 hits
    InterProiView protein in InterPro
    IPR023213 CAT-like_dom_sf
    IPR003480 Transferase
    PfamiView protein in Pfam
    PF02458 Transferase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9SRQ2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDHQVSLPQS TTTGLSFKVH RQQRELVTPA KPTPRELKPL SDIDDQQGLR
    60 70 80 90 100
    FQIPVIFFYR PNLSSDLDPV QVIKKALADA LVYYYPFAGR LRELSNRKLA
    110 120 130 140 150
    VDCTGEGVLF IEAEADVALA ELEEADALLP PFPFLEELLF DVEGSSDVLN
    160 170 180 190 200
    TPLLLVQVTR LKCCGFIFAL RFNHTMTDGA GLSLFLKSLC ELACGLHAPS
    210 220 230 240 250
    VPPVWNRHLL TVSASEARVT HTHREYDDQV GIDVVATGHP LVSRSFFFRA
    260 270 280 290 300
    EEISAIRKLL PPDLHNTSFE ALSSFLWRCR TIALNPDPNT EMRLTCIINS
    310 320 330 340 350
    RSKLRNPPLE PGYYGNVFVI PAAIATARDL IEKPLEFALR LIQETKSSVT
    360 370 380 390 400
    EDYVRSVTAL MATRGRPMFV ASGNYIISDL RHFDLGKIDF GPWGKPVYGG
    410 420 430 440 450
    TAKAGIALFP GVSFYVPFKN KKGETGTVVA ISLPVRAMET FVAELNGVLN

    VSKG
    Length:454
    Mass (Da):50,290
    Last modified:May 1, 2000 - v1
    Checksum:iC684495199FCCFB2
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti338A → V in AAM61186 (Ref. 5) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF500201 mRNA Translation: AAN09797.1
    AC009895 Genomic DNA Translation: AAF01587.1
    CP002686 Genomic DNA Translation: AEE73949.1
    AY084623 mRNA Translation: AAM61186.1
    RefSeqiNP_186998.1, NM_111219.3
    UniGeneiAt.40974

    Genome annotation databases

    EnsemblPlantsiAT3G03480.1; AT3G03480.1; AT3G03480
    GeneIDi821249
    GrameneiAT3G03480.1; AT3G03480.1; AT3G03480
    KEGGiath:AT3G03480

    Similar proteinsi

    Entry informationi

    Entry nameiCHAT_ARATH
    AccessioniPrimary (citable) accession number: Q9SRQ2
    Secondary accession number(s): Q8LFV6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: May 1, 2000
    Last modified: April 25, 2018
    This is version 92 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health