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Entry version 153 (08 May 2019)
Sequence version 2 (19 Sep 2003)
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Protein

Endoribonuclease Dicer homolog 1

Gene

DCL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Functions with DRB1/HYL1 and SERRATE proteins for accurate pri-miRNAs to miRNAs processing. Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved in the processing of natural siRNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving 24 nucleotide nat-siRNAs into 21 nucleotide nat-siRNAs. Can produce RDR6-dependent endogenous ta-siRNAs derived from TAS1 and TAS2. Required for the production of 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). Acts redundantly with DICER-LIKE 3 (DCL3) to promote flowering via repression of FLOWERING LOCUS C (FLC). Represses antiviral RNA silencing through negative regulation of the expression of DCL4 and DCL3.7 Publications

Miscellaneous

Expression in the early embryo is from the maternally contributed genome.

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi1597Magnesium or manganeseBy similarity1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei1689Important for activityBy similarity1
Metal bindingi1693Magnesium or manganeseBy similarity1
Metal bindingi1696Magnesium or manganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi269 – 276ATPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: InterPro
  • helicase activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ribonuclease III activity Source: GO_Central
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionEndonuclease, Helicase, Hydrolase, Nuclease, RNA-binding
Biological processRNA-mediated gene silencing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Endoribonuclease Dicer homolog 1 (EC:3.1.26.-)
Alternative name(s):
Dicer-like protein 1
Short name:
AtDCL1
Protein ABNORMAL SUSPENSOR 1
Protein CARPEL FACTORY
Protein SHORT INTEGUMENTS 1
Protein SUSPENSOR 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DCL1
Synonyms:ASU1, CAF SIN1, SUS1
Ordered Locus Names:At1g01040
ORF Names:T25K16.4
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

Organism-specific databases

Arabidopsis Information Portal

More...
Araporti
AT1G01040

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Embryonic lethality in sus-1 mutant. Weaker mutant (caf-1) also exists. Mutant caf-1 produces extra whorls of stamens, indefinite number of carpels and show an absence of axillary inflorescence meristems and abnormally shaped leaves and floral organs.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi395E → K in dcl1-13; early-flowering and decreased number of leaves. Suppresses hyl1 mutant phenotype. 1 Publication1
Mutagenesisi415P → S in sin1-1; impaired reproductive development. 1 Publication1
Mutagenesisi431I → K in sin1-2; impaired reproductive development. 1 Publication1
Mutagenesisi1837 – 1843NDICLRK → IAEIDPG in caf-1; converts the floral meristem to an indeterminate state. 1 Publication7
Mutagenesisi1844 – 1909Missing in caf-1; converts the floral meristem to an indeterminate state. 1 PublicationAdd BLAST66

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001804721 – 1909Endoribonuclease Dicer homolog 1Add BLAST1909

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9SP32

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Highly expressed in flowers and seeds and detected in leaves and stems. Found in ovule integuments, inflorescence and floral meristems, stigma of flowers until just before pollination, vasculature of the funiculus, and embryo.2 Publications

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Detected in the embryo, but not in the suspensor, up to the globular stage.1 Publication

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9SP32 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q9SP32 AT

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts (via N-terminus) with DDL. Interacts (via DRBM domains) with DRB1, DRB2 and DRB5. May interact with AGO1 or AGO10 through their common PAZ domains (By similarity).By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
24809, 10 interactors

Database of interacting proteins

More...
DIPi
DIP-33454N

Protein interaction database and analysis system

More...
IntActi
Q9SP32, 6 interactors

STRING: functional protein association networks

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STRINGi
3702.AT1G01040.2

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11909
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LRSNMR-A1837-1907[»]

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9SP32

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini256 – 433Helicase ATP-bindingAdd BLAST178
Domaini651 – 812Helicase C-terminalAdd BLAST162
Domaini840 – 935Dicer dsRNA-binding foldAdd BLAST96
Domaini1180 – 1318PAZAdd BLAST139
Domaini1342 – 1518RNase III 1Add BLAST177
Domaini1559 – 1707RNase III 2Add BLAST149
Domaini1733 – 1796DRBM 1Add BLAST64
Domaini1831 – 1906DRBM 2Add BLAST76

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi378 – 381DECH box4

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0701 Eukaryota
COG0571 LUCA
COG1111 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000239675

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q9SP32

KEGG Orthology (KO)

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KOi
K11592

Database for complete collections of gene phylogenies

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PhylomeDBi
Q9SP32

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00048 DSRM, 1 hit
cd00079 HELICc, 1 hit
cd00593 RIBOc, 2 hits

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.10.1520.10, 1 hit
3.30.160.380, 1 hit

HAMAP database of protein families

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HAMAPi
MF_00104 RNase_III, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR038248 Dicer_dimer_sf
IPR005034 Dicer_dimerisation_dom
IPR014720 dsRBD_dom
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR011907 RNase_III
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03368 Dicer_dimer, 1 hit
PF00271 Helicase_C, 1 hit
PF02170 PAZ, 1 hit
PF04851 ResIII, 1 hit
PF00636 Ribonuclease_3, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00487 DEXDc, 1 hit
SM00358 DSRM, 2 hits
SM00490 HELICc, 1 hit
SM00949 PAZ, 1 hit
SM00535 RIBOc, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF101690 SSF101690, 1 hit
SSF52540 SSF52540, 1 hit
SSF69065 SSF69065, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51327 DICER_DSRBF, 1 hit
PS50137 DS_RBD, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50821 PAZ, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 1 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoform i produced by alternative splicing. AlignAdd to basket
Note: A number of isoforms are produced. According to EST sequences.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9SP32-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVMEDEPREA TIKPSYWLDA CEDISCDLID DLVSEFDPSS VAVNESTDEN
60 70 80 90 100
GVINDFFGGI DHILDSIKNG GGLPNNGVSD TNSQINEVTV TPQVIAKETV
110 120 130 140 150
KENGLQKNGG KRDEFSKEEG DKDRKRARVC SYQSERSNLS GRGHVNNSRE
160 170 180 190 200
GDRFMNRKRT RNWDEAGNNK KKRECNNYRR DGRDREVRGY WERDKVGSNE
210 220 230 240 250
LVYRSGTWEA DHERDVKKVS GGNRECDVKA EENKSKPEER KEKVVEEQAR
260 270 280 290 300
RYQLDVLEQA KAKNTIAFLE TGAGKTLIAI LLIKSVHKDL MSQNRKMLSV
310 320 330 340 350
FLVPKVPLVY QQAEVIRNQT CFQVGHYCGE MGQDFWDSRR WQREFESKQV
360 370 380 390 400
LVMTAQILLN ILRHSIIRME TIDLLILDEC HHAVKKHPYS LVMSEFYHTT
410 420 430 440 450
PKDKRPAIFG MTASPVNLKG VSSQVDCAIK IRNLETKLDS TVCTIKDRKE
460 470 480 490 500
LEKHVPMPSE IVVEYDKAAT MWSLHETIKQ MIAAVEEAAQ ASSRKSKWQF
510 520 530 540 550
MGARDAGAKD ELRQVYGVSE RTESDGAANL IHKLRAINYT LAELGQWCAY
560 570 580 590 600
KVGQSFLSAL QSDERVNFQV DVKFQESYLS EVVSLLQCEL LEGAAAEKVA
610 620 630 640 650
AEVGKPENGN AHDEMEEGEL PDDPVVSGGE HVDEVIGAAV ADGKVTPKVQ
660 670 680 690 700
SLIKLLLKYQ HTADFRAIVF VERVVAALVL PKVFAELPSL SFIRCASMIG
710 720 730 740 750
HNNSQEMKSS QMQDTISKFR DGHVTLLVAT SVAEEGLDIR QCNVVMRFDL
760 770 780 790 800
AKTVLAYIQS RGRARKPGSD YILMVERGNV SHAAFLRNAR NSEETLRKEA
810 820 830 840 850
IERTDLSHLK DTSRLISIDA VPGTVYKVEA TGAMVSLNSA VGLVHFYCSQ
860 870 880 890 900
LPGDRYAILR PEFSMEKHEK PGGHTEYSCR LQLPCNAPFE ILEGPVCSSM
910 920 930 940 950
RLAQQAVCLA ACKKLHEMGA FTDMLLPDKG SGQDAEKADQ DDEGEPVPGT
960 970 980 990 1000
ARHREFYPEG VADVLKGEWV SSGKEVCESS KLFHLYMYNV RCVDFGSSKD
1010 1020 1030 1040 1050
PFLSEVSEFA ILFGNELDAE VLSMSMDLYV ARAMITKASL AFKGSLDITE
1060 1070 1080 1090 1100
NQLSSLKKFH VRLMSIVLDV DVEPSTTPWD PAKAYLFVPV TDNTSMEPIK
1110 1120 1130 1140 1150
GINWELVEKI TKTTAWDNPL QRARPDVYLG TNERTLGGDR REYGFGKLRH
1160 1170 1180 1190 1200
NIVFGQKSHP TYGIRGAVAS FDVVRASGLL PVRDAFEKEV EEDLSKGKLM
1210 1220 1230 1240 1250
MADGCMVAED LIGKIVTAAH SGKRFYVDSI CYDMSAETSF PRKEGYLGPL
1260 1270 1280 1290 1300
EYNTYADYYK QKYGVDLNCK QQPLIKGRGV SYCKNLLSPR FEQSGESETV
1310 1320 1330 1340 1350
LDKTYYVFLP PELCVVHPLS GSLIRGAQRL PSIMRRVESM LLAVQLKNLI
1360 1370 1380 1390 1400
SYPIPTSKIL EALTAASCQE TFCYERAELL GDAYLKWVVS RFLFLKYPQK
1410 1420 1430 1440 1450
HEGQLTRMRQ QMVSNMVLYQ FALVKGLQSY IQADRFAPSR WSAPGVPPVF
1460 1470 1480 1490 1500
DEDTKDGGSS FFDEEQKPVS EENSDVFEDG EMEDGELEGD LSSYRVLSSK
1510 1520 1530 1540 1550
TLADVVEALI GVYYVEGGKI AANHLMKWIG IHVEDDPDEV DGTLKNVNVP
1560 1570 1580 1590 1600
ESVLKSIDFV GLERALKYEF KEKGLLVEAI THASRPSSGV SCYQRLEFVG
1610 1620 1630 1640 1650
DAVLDHLITR HLFFTYTSLP PGRLTDLRAA AVNNENFARV AVKHKLHLYL
1660 1670 1680 1690 1700
RHGSSALEKQ IREFVKEVQT ESSKPGFNSF GLGDCKAPKV LGDIVESIAG
1710 1720 1730 1740 1750
AIFLDSGKDT TAAWKVFQPL LQPMVTPETL PMHPVRELQE RCQQQAEGLE
1760 1770 1780 1790 1800
YKASRSGNTA TVEVFIDGVQ VGVAQNPQKK MAQKLAARNA LAALKEKEIA
1810 1820 1830 1840 1850
ESKEKHINNG NAGEDQGENE NGNKKNGHQP FTRQTLNDIC LRKNWPMPSY
1860 1870 1880 1890 1900
RCVKEGGPAH AKRFTFGVRV NTSDRGWTDE CIGEPMPSVK KAKDSAAVLL

LELLNKTFS
Length:1,909
Mass (Da):213,574
Last modified:September 19, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iEAA944F0C0C81D4C
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F4HQG6F4HQG6_ARATH
Dicer-like 1
DCL1 ABNORMAL SUSPENSOR 1, ASU1, ATDCL1, CAF, CARPEL FACTORY
1,910Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAF26461 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti148S → F in AAF03534 (PubMed:10556049).Curated1
Sequence conflicti988Y → H in AAF03534 (PubMed:10556049).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AF187317 mRNA Translation: AAF03534.1
AF292940 mRNA Translation: AAG38019.1
AF292941 Genomic DNA Translation: AAG38020.1
AC007323 Genomic DNA Translation: AAF26461.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE27220.1

NCBI Reference Sequences

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RefSeqi
NP_171612.1, NM_099986.4 [Q9SP32-1]

Genome annotation databases

Ensembl plant genome annotation project

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EnsemblPlantsi
AT1G01040.1; AT1G01040.1; AT1G01040 [Q9SP32-1]

Database of genes from NCBI RefSeq genomes

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GeneIDi
839574

Gramene; a comparative resource for plants

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Gramenei
AT1G01040.1; AT1G01040.1; AT1G01040 [Q9SP32-1]

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
ath:AT1G01040

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187317 mRNA Translation: AAF03534.1
AF292940 mRNA Translation: AAG38019.1
AF292941 Genomic DNA Translation: AAG38020.1
AC007323 Genomic DNA Translation: AAF26461.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE27220.1
RefSeqiNP_171612.1, NM_099986.4 [Q9SP32-1]

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LRSNMR-A1837-1907[»]
SMRiQ9SP32
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24809, 10 interactors
DIPiDIP-33454N
IntActiQ9SP32, 6 interactors
STRINGi3702.AT1G01040.2

Proteomic databases

PaxDbiQ9SP32

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G01040.1; AT1G01040.1; AT1G01040 [Q9SP32-1]
GeneIDi839574
GrameneiAT1G01040.1; AT1G01040.1; AT1G01040 [Q9SP32-1]
KEGGiath:AT1G01040

Organism-specific databases

AraportiAT1G01040

Phylogenomic databases

eggNOGiKOG0701 Eukaryota
COG0571 LUCA
COG1111 LUCA
HOGENOMiHOG000239675
InParanoidiQ9SP32
KOiK11592
PhylomeDBiQ9SP32

Miscellaneous databases

Protein Ontology

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PROi
PR:Q9SP32

Gene expression databases

ExpressionAtlasiQ9SP32 baseline and differential
GenevisibleiQ9SP32 AT

Family and domain databases

CDDicd00048 DSRM, 1 hit
cd00079 HELICc, 1 hit
cd00593 RIBOc, 2 hits
Gene3Di1.10.1520.10, 1 hit
3.30.160.380, 1 hit
HAMAPiMF_00104 RNase_III, 1 hit
InterProiView protein in InterPro
IPR038248 Dicer_dimer_sf
IPR005034 Dicer_dimerisation_dom
IPR014720 dsRBD_dom
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR011907 RNase_III
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF03368 Dicer_dimer, 1 hit
PF00271 Helicase_C, 1 hit
PF02170 PAZ, 1 hit
PF04851 ResIII, 1 hit
PF00636 Ribonuclease_3, 2 hits
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00358 DSRM, 2 hits
SM00490 HELICc, 1 hit
SM00949 PAZ, 1 hit
SM00535 RIBOc, 2 hits
SUPFAMiSSF101690 SSF101690, 1 hit
SSF52540 SSF52540, 1 hit
SSF69065 SSF69065, 2 hits
PROSITEiView protein in PROSITE
PS51327 DICER_DSRBF, 1 hit
PS50137 DS_RBD, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50821 PAZ, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDCL1_ARATH
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9SP32
Secondary accession number(s): Q9FDY6, Q9MAN0
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: May 8, 2019
This is version 153 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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