UniProtKB - Q9SNX8 (GAPN_APIGR)
Protein
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene
N/A
Organism
Apium graveolens (Celery)
Status
Functioni
Important as a means of generating NADPH for biosynthetic reactions. May be a main source of cytosolic NADPH for mannitol biosynthesis in leaves.1 Publication
Catalytic activityi
- EC:1.2.1.9
Activity regulationi
Competitive inhibition by NADPH, 3-phospho-D-glycerate and ATP.1 Publication
Kineticsi
Measured at pH 7.7 and 30 degrees Celsius for all experiments.
- KM=6.8 µM for NADP+1 Publication
- KM=29 µM for D-glyceraldehyde 3-phosphate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 116 | SubstrateBy similarity | 1 | |
Sitei | 169 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 192 | NADPBy similarity | 1 | |
Binding sitei | 195 | NADPBy similarity | 1 | |
Binding sitei | 230 | NADPBy similarity | 1 | |
Active sitei | 264 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 298 | NucleophilePROSITE-ProRule annotation | 1 | |
Binding sitei | 391 | NADPBy similarity | 1 | |
Binding sitei | 451 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 245 – 249 | NADBy similarity | 5 |
GO - Molecular functioni
- glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity Source: UniProtKB-EC
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Stress response |
Ligand | NADP |
Enzyme and pathway databases
SABIO-RKi | Q9SNX8 |
Names & Taxonomyi
Protein namesi | Recommended name: NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.9)Alternative name(s): Glyceraldehyde-3-phosphate dehydrogenase [NADP(+)] Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase Triosephosphate dehydrogenase nr-G3PDH |
Organismi | Apium graveolens (Celery) |
Taxonomic identifieri | 4045 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Apieae › Apium |
Subcellular locationi
Cytosol
Cytosol
- cytosol Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000256065 | 1 – 496 | NADP-dependent glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST | 496 |
Proteomic databases
PRIDEi | Q9SNX8 |
Expressioni
Developmental stagei
Expression increases during leaf development. Not expressed in senescent leaves.1 Publication
Inductioni
By salt stress.1 Publication
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 169 – 170 | Substrate bindingBy similarity | 2 | |
Regioni | 297 – 299 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the aldehyde dehydrogenase family.Curated
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SNX8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGSGVYADI IEGDVFKYYS DGEWKKSSSG KSVAIINPTT RMTQFKVQAC
60 70 80 90 100
TQEEVNKAME TAKKVQKQWA KTPLWKRAEL LHKAAAILKE HKAAIADCLV
110 120 130 140 150
KEIAKPAKDS VTEVVRSGDL VSYCAEEGVR LLGEGKFLVS DSFPGNERTK
160 170 180 190 200
YCLTSKIPLG VILAIPPFNY PVNLAVSKIG PALIAGNALV LKPPTQGAVA
210 220 230 240 250
CLHMVHCFHL AGFPKGLISC ITGKGSEIGD FLTMHPGVNC ISFTGGDTGI
260 270 280 290 300
AISKKAGMVP LQMELGGKDA CIVLEDADLD LVASNVIKGG FSYSGQRCTA
310 320 330 340 350
IKVILVMQSV ADTLVEKVNA KVAKLTVGPP EDNSDITPVV SESSANFIEG
360 370 380 390 400
LVKDAKEKGA TFCQEYKREG NLIWPLLLDN VKPDMRIAWE EPFGPILPVI
410 420 430 440 450
RINSAEEGIH HCNASNFGLQ GCVFTRDINK AMLISDAMES GTIQINSAPA
460 470 480 490
RGPDHFPFQG LKDSGIGSQG ITNSINMMTK IKTTVINLPS PSYTMG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF196292 mRNA Translation: AAF08296.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF196292 mRNA Translation: AAF08296.1 |
3D structure databases
SMRi | Q9SNX8 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q9SNX8 |
Enzyme and pathway databases
SABIO-RKi | Q9SNX8 |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GAPN_APIGR | |
Accessioni | Q9SNX8Primary (citable) accession number: Q9SNX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 11, 2019 | |
This is version 76 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |