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Protein

Malate dehydrogenase, chloroplastic

Gene

At3g47520

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments (Probable). Plays a key role in the metabolism of dark chloroplasts and non-green plastids. Essential for embryo viability (PubMed:24198233, PubMed:24453164). Plays an essential role in heterotrophic metabolism in embryos, and autotrophic metabolism in photosynthetic tissues as well (PubMed:24453164).1 Publication2 Publications

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115NADBy similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei168SubstrateBy similarity1
Binding sitei175NADBy similarity1
Binding sitei200SubstrateBy similarity1
Binding sitei234SubstrateBy similarity1
Active sitei258Proton acceptorBy similarity1
Binding sitei309NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 95NADBy similarity7
Nucleotide bindingi198 – 200NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: TAIR
  • NAD(P)+ transhydrogenase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciARA:AT3G47520-MONOMER
MetaCyc:AT3G47520-MONOMER
ReactomeiR-ATH-70263 Gluconeogenesis
R-ATH-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, chloroplasticCurated (EC:1.1.1.37)
Alternative name(s):
Chloroplastic malate dehydrogenase1 Publication
Short name:
Chloroplastic MDHCurated
Short name:
cpNAD-MDH1 Publication
Plastidic NAD-dependent malate dehydrogenaseCurated
Short name:
pNAD-MDHCurated
Gene namesi
Ordered Locus Names:At3g47520
ORF Names:F1P2.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G47520
TAIRilocus:2079177 AT3G47520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality when homozygous due to embryo development arrest at globular stage.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 80ChloroplastSequence analysisAdd BLAST80
ChainiPRO_000022414981 – 403Malate dehydrogenase, chloroplasticAdd BLAST323

Proteomic databases

PaxDbiQ9SN86
PRIDEiQ9SN86

Expressioni

Tissue specificityi

Expressed in rosette leaves (PubMed:20876337). Expressed in meristematic regions of roots and shoots, cotyledons, young leaves, trichomes, stamen, pollen, tapetum, gynoecium and ovules (PubMed:24198233).2 Publications

Developmental stagei

During pollen development, expressed in mature pollen grains and early in pollen-tube growth. Not expressed in immature pollen grains and fully grown pollen tubes (PubMed:24198233). During embryo development, expressed from the heart stage to mature embryo. Not expressed at the beginning of embryo development up to the globular stage (PubMed:24198233, PubMed:24453164).2 Publications

Gene expression databases

ExpressionAtlasiQ9SN86 baseline and differential
GenevisibleiQ9SN86 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi9226, 2 interactors
STRINGi3702.AT3G47520.1

Structurei

3D structure databases

ProteinModelPortaliQ9SN86
SMRiQ9SN86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1494 Eukaryota
COG0039 LUCA
HOGENOMiHOG000213792
InParanoidiQ9SN86
KOiK00026
OMAiGYLPAND
OrthoDBiEOG093609FL
PhylomeDBiQ9SN86

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SN86-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATATSASLF STVSSSYSKA SSIPHSRLQS VKFNSVPSFT GLKSTSLISG
60 70 80 90 100
SDSSSLAKTL RGSVTKAQTS DKKPYGFKIN ASYKVAVLGA AGGIGQPLSL
110 120 130 140 150
LIKMSPLVST LHLYDIANVK GVAADLSHCN TPSQVRDFTG PSELADCLKD
160 170 180 190 200
VNVVVIPAGV PRKPGMTRDD LFNINANIVK TLVEAVAENC PNAFIHIISN
210 220 230 240 250
PVNSTVPIAA EVLKKKGVYD PKKLFGVTTL DVVRANTFVS QKKNLKLIDV
260 270 280 290 300
DVPVIGGHAG ITILPLLSKT KPSVNFTDEE IQELTVRIQN AGTEVVDAKA
310 320 330 340 350
GAGSATLSMA YAAARFVESS LRALDGDGDV YECSFVESTL TDLPFFASRV
360 370 380 390 400
KIGKNGLEAV IESDLQGLTE YEQKALEALK VELKASIDKG VAFANKPAAA

AAN
Length:403
Mass (Da):42,406
Last modified:May 1, 2000 - v1
Checksum:i65B54DE66392D229
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57A → T in CAA74320 (PubMed:9774405).Curated1
Sequence conflicti66K → N in CAA74320 (PubMed:9774405).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13987 mRNA Translation: CAA74320.1
AL132955 Genomic DNA Translation: CAB61978.1
CP002686 Genomic DNA Translation: AEE78292.1
AY128281 mRNA Translation: AAM91090.1
BT000621 mRNA Translation: AAN18188.1
PIRiT45712
T51862
RefSeqiNP_190336.1, NM_114620.3
UniGeneiAt.20474

Genome annotation databases

EnsemblPlantsiAT3G47520.1; AT3G47520.1; AT3G47520
GeneIDi823906
GrameneiAT3G47520.1; AT3G47520.1; AT3G47520
KEGGiath:AT3G47520

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13987 mRNA Translation: CAA74320.1
AL132955 Genomic DNA Translation: CAB61978.1
CP002686 Genomic DNA Translation: AEE78292.1
AY128281 mRNA Translation: AAM91090.1
BT000621 mRNA Translation: AAN18188.1
PIRiT45712
T51862
RefSeqiNP_190336.1, NM_114620.3
UniGeneiAt.20474

3D structure databases

ProteinModelPortaliQ9SN86
SMRiQ9SN86
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9226, 2 interactors
STRINGi3702.AT3G47520.1

Proteomic databases

PaxDbiQ9SN86
PRIDEiQ9SN86

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G47520.1; AT3G47520.1; AT3G47520
GeneIDi823906
GrameneiAT3G47520.1; AT3G47520.1; AT3G47520
KEGGiath:AT3G47520

Organism-specific databases

AraportiAT3G47520
TAIRilocus:2079177 AT3G47520

Phylogenomic databases

eggNOGiKOG1494 Eukaryota
COG0039 LUCA
HOGENOMiHOG000213792
InParanoidiQ9SN86
KOiK00026
OMAiGYLPAND
OrthoDBiEOG093609FL
PhylomeDBiQ9SN86

Enzyme and pathway databases

BioCyciARA:AT3G47520-MONOMER
MetaCyc:AT3G47520-MONOMER
ReactomeiR-ATH-70263 Gluconeogenesis
R-ATH-71403 Citric acid cycle (TCA cycle)

Miscellaneous databases

PROiPR:Q9SN86

Gene expression databases

ExpressionAtlasiQ9SN86 baseline and differential
GenevisibleiQ9SN86 AT

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMDHP_ARATH
AccessioniPrimary (citable) accession number: Q9SN86
Secondary accession number(s): O81844
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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