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Entry version 128 (18 Sep 2019)
Sequence version 1 (01 May 2000)
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Protein

Alpha-aminoadipic semialdehyde synthase

Gene

LKR/SDH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively. Negatively regulates free Lys accumulation in seeds.3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

The LKR activity is stimulated by NaCl.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=5.180 mM for lysine (isoform Long at pH 7.5 and 30 degrees Celsius)1 Publication
  2. KM=0.272 mM for alpha-ketoglutarate (isoform Long at pH 7.5 and 30 degrees Celsius)1 Publication
  3. KM=0.044 mM for NADPH (isoform Long at pH 7.5 and 30 degrees Celsius)1 Publication
  4. KM=0.063 mM for saccharopine (isoform Long at pH 7 and 30 degrees Celsius)1 Publication
  5. KM=0.035 mM for saccharopine (isoform Long at pH 9 and 30 degrees Celsius)1 Publication
  6. KM=0.130 mM for saccharopine (isoform Short at pH 7 and 30 degrees Celsius)1 Publication
  7. KM=0.050 mM for saccharopine (isoform Short at pH 9 and 30 degrees Celsius)1 Publication
  8. KM=0.374 mM for NAD (isoform Long at pH 7 and 30 degrees Celsius)1 Publication
  9. KM=0.698 mM for NAD (isoform Long at pH 9 and 30 degrees Celsius)1 Publication
  10. KM=0.333 mM for NAD (isoform Short at pH 7 and 30 degrees Celsius)1 Publication
  11. KM=0.759 mM for NAD (isoform Short at pH 9 and 30 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.5 for LKR activity of isoform Long, and 9 for SDH activity of both isoforms Long and Short.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-lysine degradation via saccharopine pathway

    This protein is involved in step 1 and 2 of the subpathway that synthesizes glutaryl-CoA from L-lysine.
    Proteins known to be involved in the 6 steps of the subpathway in this organism are:
    1. Alpha-aminoadipic semialdehyde synthase (LKR/SDH)
    2. Alpha-aminoadipic semialdehyde synthase (LKR/SDH)
    3. no protein annotated in this organism
    4. no protein annotated in this organism
    5. no protein annotated in this organism
    6. Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 2, mitochondrial (At4g26910), Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex 1, mitochondrial (At5g55070)
    This subpathway is part of the pathway L-lysine degradation via saccharopine pathway, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutaryl-CoA from L-lysine, the pathway L-lysine degradation via saccharopine pathway and in Amino-acid degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei730SaccharopineBy similarity1
    Binding sitei830SaccharopineBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi729 – 731NADPBy similarity3

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionMultifunctional enzyme, Oxidoreductase
    LigandNAD, NADP

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    ARA:AT4G33150-MONOMER

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    Q9SMZ4

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00868;UER00835
    UPA00868;UER00836

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Alpha-aminoadipic semialdehyde synthase
    Alternative name(s):
    cAt-LKR/SDH
    Short name:
    LKR/SDH
    Including the following 2 domains:
    Lysine ketoglutarate reductase (EC:1.5.1.8)
    Short name:
    LKR
    Saccharopine dehydrogenase (EC:1.5.1.9)
    Alternative name(s):
    cAt-SDH
    Short name:
    SDH
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:LKR/SDH
    Synonyms:LKR, SDH
    Ordered Locus Names:At4g33150
    ORF Names:F4I10.80
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 4

    Organism-specific databases

    Arabidopsis Information Portal

    More...
    Araporti
    AT4G33150

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi238T → A or D: No effect on LKR and SDH activity. 1 Publication1
    Mutagenesisi407S → A: No effect on LKR and SDH activity. 1 Publication1
    Mutagenesisi407S → D: No LKR activity, but no effect on SDH activity. 1 Publication1
    Mutagenesisi458S → A: Reduced LKR activity, but no effect on SDH activity. 1 Publication1
    Mutagenesisi458S → D: No effect on LKR and SDH activity. 1 Publication1
    Mutagenesisi551 – 554NEDY → IEGR: Loss of LKR activity stimulation by NaCl. 1 Publication4

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002260701 – 1064Alpha-aminoadipic semialdehyde synthaseAdd BLAST1064

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei238Phosphothreonine1 Publication1
    Modified residuei458Phosphoserine1 Publication1

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Phosphorylation of Ser-458 seems important for the LKR activity.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    Q9SMZ4

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q9SMZ4

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    Q9SMZ4

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Ubiquitous, with higher levels in flowers. Isoform Long is mostly present in young leaves, cotyledons, root tips and mature root parts. Whereas isoform Short is mostly expressed in cotyledons and at low levels in all root parts.2 Publications

    <p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

    In flowers, confined to ovules and vascular tissue of anther filament. In developing and mature seeds, expressed in embryo and the outer layers of the endosperm.1 Publication

    <p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Lysine, Sugar starvation, ABA and MeJA induce isoform Long, but not isoform Short (at protein level). Nitrogen starvation repress isoform Long, but not isoform Short (at protein level). Isoform Long and isoform Short are both slightly induced by NaCl and drought stress, but repressed by sugars.2 Publications

    Gene expression databases

    ExpressionAtlas, Differential and Baseline Expression

    More...
    ExpressionAtlasi
    Q9SMZ4 baseline and differential

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    3702.AT4G33150.2

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q9SMZ4

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni24 – 445Lysine-ketoglutarate reductaseAdd BLAST422
    Regioni583 – 1064Saccharopine dehydrogenaseAdd BLAST482
    Regioni703 – 704Saccharopine bindingBy similarity2
    Regioni852 – 854Saccharopine bindingBy similarity3

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    In the N-terminal section; belongs to the AlaDH/PNT family.Curated
    In the C-terminal section; belongs to the saccharopine dehydrogenase family.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG0172 Eukaryota
    COG1748 LUCA

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q9SMZ4

    KEGG Orthology (KO)

    More...
    KOi
    K14157

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    RHIKGIT

    Database of Orthologous Groups

    More...
    OrthoDBi
    498994at2759

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    Q9SMZ4

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd12144 SDH_N_domain, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR007886 AlaDH/PNT_N
    IPR007698 AlaDH/PNT_NAD(H)-bd
    IPR007545 LOR/SDH_bifunc_enz_cons_dom
    IPR036291 NAD(P)-bd_dom_sf
    IPR032095 Sacchrp_dh_C
    IPR005097 Sacchrp_dh_NADP

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF05222 AlaDh_PNT_N, 1 hit
    PF04455 Saccharop_dh_N, 1 hit
    PF16653 Sacchrp_dh_C, 1 hit
    PF03435 Sacchrp_dh_NADP, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM01002 AlaDh_PNT_C, 1 hit
    SM01003 AlaDh_PNT_N, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51735 SSF51735, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative initiation. AlignAdd to basket

    This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

    Isoform Long (identifier: Q9SMZ4-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MNSNGHEEEK KLGNGVVGIL AETVNKWERR TPLTPSHCAR LLHGGKDRTG
    60 70 80 90 100
    ISRIVVQPSA KRIHHDALYE DVGCEISDDL SDCGLILGIK QPELEMILPE
    110 120 130 140 150
    RAYAFFSHTH KAQKENMPLL DKILSERVTL CDYELIVGDH GKRLLAFGKY
    160 170 180 190 200
    AGRAGLVDFL HGLGQRKLIL GYSTPFLSLG ASYMYSSLAA AKAAVISVGE
    210 220 230 240 250
    EIASQGLPLG ICPLVFVFTG TGNVSLGAQE IFKLLPHTFV EPSKLPELFV
    260 270 280 290 300
    KDKGISQNGI STKRVYQVYG CIITSQDMVE HKDPSKSFDK ADYYAHPEHY
    310 320 330 340 350
    NPVFHEKISP YTSVLVNCMY WEKRFPCLLS TKQLQDLTKK GLPLVGICDI
    360 370 380 390 400
    TCDIGGSIEF VNRATLIDSP FFRFNPSNNS YYDDMDGDGV LCMAVDILPT
    410 420 430 440 450
    EFAKEASQHF GDILSGFVGS LASMTEISDL PAHLKRACIS YRGELTSLYE
    460 470 480 490 500
    YIPRMRKSNP EEAQDNIIAN GVSSQRTFNI LVSLSGHLFD KFLINEALDM
    510 520 530 540 550
    IEAAGGSFHL AKCELGQSAD AESYSELEVG ADDKRVLDQI IDSLTRLANP
    560 570 580 590 600
    NEDYISPHRE ANKISLKIGK VQQENEIKEK PEMTKKSGVL ILGAGRVCRP
    610 620 630 640 650
    AADFLASVRT ISSQQWYKTY FGADSEEKTD VHVIVASLYL KDAKETVEGI
    660 670 680 690 700
    SDVEAVRLDV SDSESLLKYV SQVDVVLSLL PASCHAVVAK TCIELKKHLV
    710 720 730 740 750
    TASYVDDETS MLHEKAKSAG ITILGEMGLD PGIDHMMAMK MINDAHIKKG
    760 770 780 790 800
    KVKSFTSYCG GLPSPAAANN PLAYKFSWNP AGAIRAGQNP AKYKSNGDII
    810 820 830 840 850
    HVDGKNLYDS AARFRVPNLP AFALECFPNR DSLVYGEHYG IESEATTIFR
    860 870 880 890 900
    GTLRYEGFSM IMATLSKLGF FDSEANQVLS TGKRITFGAL LSNILNKDAD
    910 920 930 940 950
    NESEPLAGEE EISKRIIKLG HSKETAAKAA KTIVFLGFNE EREVPSLCKS
    960 970 980 990 1000
    VFDATCYLME EKLAYSGNEQ DMVLLHHEVE VEFLESKRIE KHTATLLEFG
    1010 1020 1030 1040 1050
    DIKNGQTTTA MAKTVGIPAA IGALLLIEDK IKTRGVLRPL EAEVYLPALD
    1060
    ILQAYGIKLM EKAE
    Note: Contains both LKR and SDH activities.
    Length:1,064
    Mass (Da):117,149
    Last modified:May 1, 2000 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6CA4EBDB22898C7E
    GO
    Isoform Short (identifier: Q9SMZ4-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-582: Missing.

    Note: Contains only SDH activity.
    Show »
    Length:482
    Mass (Da):52,704
    Checksum:iA249E25010D557A3
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    F4JVY8F4JVY8_ARATH
    Lysine-ketoglutarate reductase/sacc...
    LKR LYSINE-KETOGLUTARATE REDUCTASE, SACCHAROPINE DEHYDROGENASE, SDH, At4g33150, F4I10.80
    1,064Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    <p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

    The sequence AAK64010 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAK97099 differs from that shown. Chimeric cDNA. Its C-terminal part is derived from gene At1G61240 whose function is unknown. Originally thought to be an alternative splicing form of At4g33150.Curated
    The sequence AAM16268 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti167 – 169KLI → YLS in AAB53975 (PubMed:9286108).Curated3
    Sequence conflicti167 – 169KLI → YLS in AAB96825 (PubMed:9426595).Curated3
    Sequence conflicti167 – 169KLI → YLS in AAB96826 (PubMed:9426595).Curated3
    Sequence conflicti167 – 169KLI → YLS in AAK97099 (PubMed:12226495).Curated3
    Sequence conflicti324R → S in AAB53975 (PubMed:9286108).Curated1
    Sequence conflicti324R → S in AAK97099 (PubMed:12226495).Curated1
    Sequence conflicti621F → V in AAK64010 (PubMed:14593172).Curated1
    Sequence conflicti621F → V in AAM16268 (PubMed:14593172).Curated1
    Sequence conflicti715K → N in AAB53975 (PubMed:9286108).Curated1
    Sequence conflicti715K → N in AAD00700 (PubMed:9286108).Curated1
    Sequence conflicti735H → P in AAB53975 (PubMed:9286108).Curated1
    Sequence conflicti735H → P in AAD00700 (PubMed:9286108).Curated1
    Sequence conflicti739M → K in AAB53975 (PubMed:9286108).Curated1
    Sequence conflicti739M → K in AAD00700 (PubMed:9286108).Curated1
    Sequence conflicti746 – 748HIK → PIT in AAB53975 (PubMed:9286108).Curated3
    Sequence conflicti746 – 748HIK → PIT in AAD00700 (PubMed:9286108).Curated3
    Sequence conflicti765P → R in AAB53975 (PubMed:9286108).Curated1
    Sequence conflicti765P → R in AAD00700 (PubMed:9286108).Curated1
    Sequence conflicti827F → L in AAB96825 (PubMed:9426595).Curated1
    Sequence conflicti827F → L in AAB96826 (PubMed:9426595).Curated1
    Sequence conflicti1040L → F in AAB96825 (PubMed:9426595).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0186411 – 582Missing in isoform Short. 1 PublicationAdd BLAST582

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    U90522 mRNA Translation: AAB53975.1
    U90523 mRNA Translation: AAD00700.1
    U95758 Genomic DNA Translation: AAB96825.1
    U95759 mRNA Translation: AAB96826.1
    AF295389 mRNA Translation: AAK97099.1 Sequence problems.
    AL035525 Genomic DNA Translation: CAB36789.1
    AL161583 Genomic DNA Translation: CAB80032.1
    CP002687 Genomic DNA Translation: AEE86184.1
    AY039906 mRNA Translation: AAK64010.1 Different initiation.
    AY094007 mRNA Translation: AAM16268.1 Different initiation.

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    T05195

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001154283.1, NM_001160811.2 [Q9SMZ4-2]

    Genome annotation databases

    Ensembl plant genome annotation project

    More...
    EnsemblPlantsi
    AT4G33150.3; AT4G33150.3; AT4G33150 [Q9SMZ4-2]

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    829452

    Gramene; a comparative resource for plants

    More...
    Gramenei
    AT4G33150.3; AT4G33150.3; AT4G33150 [Q9SMZ4-2]

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ath:AT4G33150

    Keywords - Coding sequence diversityi

    Alternative initiation

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U90522 mRNA Translation: AAB53975.1
    U90523 mRNA Translation: AAD00700.1
    U95758 Genomic DNA Translation: AAB96825.1
    U95759 mRNA Translation: AAB96826.1
    AF295389 mRNA Translation: AAK97099.1 Sequence problems.
    AL035525 Genomic DNA Translation: CAB36789.1
    AL161583 Genomic DNA Translation: CAB80032.1
    CP002687 Genomic DNA Translation: AEE86184.1
    AY039906 mRNA Translation: AAK64010.1 Different initiation.
    AY094007 mRNA Translation: AAM16268.1 Different initiation.
    PIRiT05195
    RefSeqiNP_001154283.1, NM_001160811.2 [Q9SMZ4-2]

    3D structure databases

    SMRiQ9SMZ4
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT4G33150.2

    PTM databases

    iPTMnetiQ9SMZ4

    Proteomic databases

    PaxDbiQ9SMZ4
    PRIDEiQ9SMZ4

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G33150.3; AT4G33150.3; AT4G33150 [Q9SMZ4-2]
    GeneIDi829452
    GrameneiAT4G33150.3; AT4G33150.3; AT4G33150 [Q9SMZ4-2]
    KEGGiath:AT4G33150

    Organism-specific databases

    AraportiAT4G33150

    Phylogenomic databases

    eggNOGiKOG0172 Eukaryota
    COG1748 LUCA
    InParanoidiQ9SMZ4
    KOiK14157
    OMAiRHIKGIT
    OrthoDBi498994at2759
    PhylomeDBiQ9SMZ4

    Enzyme and pathway databases

    UniPathwayiUPA00868;UER00835
    UPA00868;UER00836
    BioCyciARA:AT4G33150-MONOMER
    SABIO-RKiQ9SMZ4

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:Q9SMZ4

    Gene expression databases

    ExpressionAtlasiQ9SMZ4 baseline and differential

    Family and domain databases

    CDDicd12144 SDH_N_domain, 1 hit
    InterProiView protein in InterPro
    IPR007886 AlaDH/PNT_N
    IPR007698 AlaDH/PNT_NAD(H)-bd
    IPR007545 LOR/SDH_bifunc_enz_cons_dom
    IPR036291 NAD(P)-bd_dom_sf
    IPR032095 Sacchrp_dh_C
    IPR005097 Sacchrp_dh_NADP
    PfamiView protein in Pfam
    PF05222 AlaDh_PNT_N, 1 hit
    PF04455 Saccharop_dh_N, 1 hit
    PF16653 Sacchrp_dh_C, 1 hit
    PF03435 Sacchrp_dh_NADP, 1 hit
    SMARTiView protein in SMART
    SM01002 AlaDh_PNT_C, 1 hit
    SM01003 AlaDh_PNT_N, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAASS_ARATH
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9SMZ4
    Secondary accession number(s): O04155
    , O04156, O04884, Q7DM71, Q947M5, Q94BT4
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: May 1, 2000
    Last modified: September 18, 2019
    This is version 128 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
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