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Protein

Probable pectinesterase/pectinesterase inhibitor 16

Gene

PME16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At5g45990), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At3g06710), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g50310), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At3g31890), Pectinesterase (PMEPCRF), Pectinesterase (At3g10720), Putative pectinesterase 10 (PME10), Putative pectinesterase 11 (PME11), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 14 (PME14), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase/pectinesterase inhibitor 18 (PME18), Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Pectinesterase 1 (PME1), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase 29 (PME29), Pectinesterase 2 (PME2), Probable pectinesterase 30 (PME30), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase/pectinesterase inhibitor 3 (PME3), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Pectinesterase 4 (PME4), Probable pectinesterase 50 (PME50), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Putative pectinesterase 52 (PME52), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Probable pectinesterase 55 (PME55), Probable pectinesterase 56 (PME56), Putative pectinesterase 57 (PME57), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Pectinesterase QRT1 (QRT1), Putative pectinesterase 63 (PME63), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Probable pectinesterase 66 (PME66), Probable pectinesterase 67 (PME67), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 8 (PME8), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At3g30550), Pectinesterase (At3g14310), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g18720), Pectinesterase (At4g15980), Pectinesterase (At3g49220), Pectinesterase (AXX17_At2g22270), Pectinesterase (AXX17_At4g38050), Uncharacterized protein (AXX17_At3g05180), Pectinesterase (AXX17_At5g47900), Pectinesterase, Pectinesterase (AXX17_At5g19650), Pectinesterase (AXX17_At3g43380), Pectinesterase (AXX17_At4g02900), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At3g10570), Pectinesterase (AXX17_At3g56460), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At1g11900), PME26 (AXX17_At3g14890), Uncharacterized protein (AXX17_At5g27870), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (AXX17_At1g04730), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At5g04430), Pectinesterase, Pectinesterase (F14I3.7), Pectinesterase (AXX17_At2g22280), Pectinesterase, Pectinesterase (AXX17_At5g52320), Pectinesterase (AXX17_At2g33420), Pectinesterase, Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At2g40510), Pectinesterase (At5g20860), Pectinesterase (AXX17_At3g26070), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g38040), Pectinesterase (AXX17_At2g33410), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At3g05190), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At4g02930), Pectinesterase (AXX17_At3g17940), Pectinesterase (AXX17_At2g45340), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (At5g26810), Pectinesterase (PME44), Pectinesterase (At1g53840)
  2. Pectate lyase (AXX17_At3g06910), Pectate lyase (At3g27400), Pectate lyase (AXX17_At3g26200), Pectate lyase (AXX17_At3g09180), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At3g49720), Pectate lyase (AXX17_At4g25610), Putative pectate lyase 14 (At4g13210), Probable pectate lyase 19 (At5g15110), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 6 (At2g02720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 12 (At3g53190), Putative pectate lyase 17 (At4g22090), Probable pectate lyase 10 (At3g24670), Probable pectate lyase 13 (PMR6), Probable pectate lyase 1 (At1g04680), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 16 (At4g22080), Probable pectate lyase 18 (At4g24780), Putative pectate lyase 21 (At5g55720), Probable pectate lyase 22 (At5g63180), Putative pectate lyase 2 (At1g11920), Putative pectate lyase 11 (At3g27400), Probable pectate lyase 15 (At4g13710), Pectate lyase (At5g09280), Pectate lyase (At3g55140), Pectate lyase, Pectate lyase (At1g14420), Pectate lyase (F11F8_12), Pectate lyase (At5g04310), Pectate lyase (AXX17_At3g47590), Pectate lyase (At3g09540), Pectate lyase (AXX17_At5g54880), Pectate lyase, Pectate lyase (AXX17_At5g08800), Pectate lyase (AXX17_At1g04020), Pectate lyase (AXX17_At1g15010), Pectate lyase (AXX17_At1g12300), Pectate lyase (At3g01270), Pectate lyase (AXX17_At2g01680), Pectate lyase (At3g55140), Pectate lyase (AXX17_At3g00310), Pectate lyase (AXX17_At5g47570), Pectate lyase (AXX17_At5g03700), Pectate lyase (AXX17_At1g30780), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At3g29900), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g14600), Pectate lyase (AXX17_At1g61710), Pectate lyase (AXX17_At5g62760), Pectate lyase (At3g07010), Pectate lyase (At3g01270), Pectate lyase, Pectate lyase (AXX17_At4g25600), Pectate lyase (At5g04310), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At4g15660), Pectate lyase (At3g53190), Pectate lyase, Pectate lyase (At4g13710), Pectate lyase (At4g13210), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g26720), Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei289Substrate; for pectinesterase activityBy similarity1
Binding sitei319Substrate; for pectinesterase activityBy similarity1
Sitei341Transition state stabilizerBy similarity1
Active sitei342Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei363Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei422Substrate; for pectinesterase activityBy similarity1
Binding sitei424Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: GO_Central
  • pectinesterase inhibitor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G43050-MONOMER
UniPathwayi
UPA00545;UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 16
Including the following 2 domains:
Pectinesterase inhibitor 16
Alternative name(s):
Pectin methylesterase inhibitor 16
Pectinesterase 16 (EC:3.1.1.11)
Short name:
PE 16
Alternative name(s):
AtPMEpcrD
Pectin methylesterase 16
Short name:
AtPME16
Gene namesi
Name:PME16
Synonyms:ARATH16
Ordered Locus Names:At2g43050
ORF Names:MFL8.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G43050
TAIRilocus:2053728 AT2G43050

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000037167334 – 518Probable pectinesterase/pectinesterase inhibitor 16Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9SKX2
PRIDEiQ9SKX2

Expressioni

Tissue specificityi

Expressed in siliques and floral stems.1 Publication

Gene expression databases

ExpressionAtlasiQ9SKX2 baseline and differential
GenevisibleiQ9SKX2 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G43050.1

Structurei

3D structure databases

ProteinModelPortaliQ9SKX2
SMRiQ9SKX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 172Pectinesterase inhibitor 16Add BLAST143
Regioni213 – 502Pectinesterase 16Add BLAST290

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 56Ser-richAdd BLAST23

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVDW Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9SKX2
OMAiCRITAES
OrthoDBiEOG0936097I
PhylomeDBiQ9SKX2

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SKX2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSSSISNH KIPNTLMFLV IVNFLYLIQT NSAVSISSNS NSHFSRFSRH
60 70 80 90 100
RSSPSSKTKQ GFLATVQESM NHALLARSLA FNLTLSHRTV QTHTFDPIHD
110 120 130 140 150
CLELLDDTLD MLSRIHADND EEDVHTWLSA ALTNQDTCEQ SLQEKSESYK
160 170 180 190 200
HGLAMDFVAR NLTGLLTSSL DLFVSVKSKH RKLLSKQEYF PTFVPSSEQR
210 220 230 240 250
RLLEAPVEEL NVDAVVAPDG SGTHKTIGEA LLSTSLASSG GRTKIYLKAG
260 270 280 290 300
TYHENINIPT KQKNVMLVGD GKGKTVIVGS RSNRGGWTTY KTATVAAMGE
310 320 330 340 350
GFIARDMTFV NNAGPKSEQA VALRVGADKS VVHRCSVEGY QDSLYTHSKR
360 370 380 390 400
QFYRETDITG TVDFIFGNSA VVFQSCNIAA RKPLPGQRNF VTAQGRSNPG
410 420 430 440 450
QNTGIAIQNC RITAESMTYL GRPWKEYSRT VVMQSFIGGS IHPSGWSPWS
460 470 480 490 500
GGFGLKSLFY GEYGNSGPGS SVSGRVKWSG CHPSLTVTEA EKFTVASFID
510
GNIWLPSTGV SFDPGLVN
Length:518
Mass (Da):56,589
Last modified:May 1, 2000 - v1
Checksum:iC5E7A903F0786604
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006224 Genomic DNA Translation: AAD22126.1
CP002685 Genomic DNA Translation: AEC10203.1
BT023433 mRNA Translation: AAY56424.1
PIRiD84861
RefSeqiNP_181833.1, NM_129866.5
UniGeneiAt.43649
At.67744
At.74784
At.74860

Genome annotation databases

EnsemblPlantsiAT2G43050.1; AT2G43050.1; AT2G43050
GeneIDi818907
GrameneiAT2G43050.1; AT2G43050.1; AT2G43050
KEGGiath:AT2G43050

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006224 Genomic DNA Translation: AAD22126.1
CP002685 Genomic DNA Translation: AEC10203.1
BT023433 mRNA Translation: AAY56424.1
PIRiD84861
RefSeqiNP_181833.1, NM_129866.5
UniGeneiAt.43649
At.67744
At.74784
At.74860

3D structure databases

ProteinModelPortaliQ9SKX2
SMRiQ9SKX2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G43050.1

Proteomic databases

PaxDbiQ9SKX2
PRIDEiQ9SKX2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43050.1; AT2G43050.1; AT2G43050
GeneIDi818907
GrameneiAT2G43050.1; AT2G43050.1; AT2G43050
KEGGiath:AT2G43050

Organism-specific databases

AraportiAT2G43050
TAIRilocus:2053728 AT2G43050

Phylogenomic databases

eggNOGiENOG410IVDW Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9SKX2
OMAiCRITAES
OrthoDBiEOG0936097I
PhylomeDBiQ9SKX2

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

BioCyciARA:AT2G43050-MONOMER

Miscellaneous databases

PROiPR:Q9SKX2

Gene expression databases

ExpressionAtlasiQ9SKX2 baseline and differential
GenevisibleiQ9SKX2 AT

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPME16_ARATH
AccessioniPrimary (citable) accession number: Q9SKX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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