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Protein

Type II inositol polyphosphate 5-phosphatase 14

Gene

IP5P14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has phosphatase activity toward PtdIns(4,5)P2, PtdIns(3,4,5)P3 and Ins(1,4,5)P3.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.1 Publication
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3,4-diphosphate + phosphate.1 Publication
D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=101 µM for PtdIns(4,5)1 Publication
  2. KM=211 µM for PtdIns(3,4,5)P31 Publication
  3. KM=395 µM for Ins(1,4,5)P31 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.1.3.36 399
    ReactomeiR-ATH-1660499 Synthesis of PIPs at the plasma membrane
    R-ATH-1855183 Synthesis of IP2, IP, and Ins in the cytosol
    R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type II inositol polyphosphate 5-phosphatase 141 Publication (EC:3.1.3.361 Publication, EC:3.1.3.861 Publication)
    Short name:
    At5PTase141 Publication
    Gene namesi
    Name:IP5P14Curated
    Synonyms:5PTASE14
    Ordered Locus Names:At2g31830Imported
    ORF Names:F20M17.13Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G31830

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003597441 – 1144Type II inositol polyphosphate 5-phosphatase 14Add BLAST1144

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki949Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiQ9SKB7
    PRIDEiQ9SKB7

    PTM databases

    iPTMnetiQ9SKB7

    Expressioni

    Tissue specificityi

    Expressed in young seedlings and flowers.1 Publication

    Inductioni

    Slightly reduced by dark treatment.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9SKB7 baseline and differential
    GenevisibleiQ9SKB7 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G31830.2

    Structurei

    3D structure databases

    ProteinModelPortaliQ9SKB7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati158 – 196WD 1Add BLAST39
    Repeati216 – 255WD 2Add BLAST40
    Repeati269 – 307WD 3Add BLAST39
    Repeati445 – 483WD 4Add BLAST39
    Repeati524 – 561WD 5Add BLAST38

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni791 – 807Catalytic 1By similarityAdd BLAST17
    Regioni870 – 885Catalytic 2By similarityAdd BLAST16

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi758 – 763Poly-Ala6

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiKOG0565 Eukaryota
    COG5411 LUCA
    HOGENOMiHOG000242921
    InParanoidiQ9SKB7
    PhylomeDBiQ9SKB7

    Family and domain databases

    Gene3Di2.130.10.10, 2 hits
    3.60.10.10, 1 hit
    InterProiView protein in InterPro
    IPR036691 Endo/exonu/phosph_ase_sf
    IPR005135 Endo/exonuclease/phosphatase
    IPR000300 IPPc
    IPR015943 WD40/YVTN_repeat-like_dom_sf
    IPR001680 WD40_repeat
    PfamiView protein in Pfam
    PF03372 Exo_endo_phos, 1 hit
    SMARTiView protein in SMART
    SM00128 IPPc, 1 hit
    SM00320 WD40, 4 hits
    SUPFAMiSSF56219 SSF56219, 2 hits

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
    Note: A number of isoforms are produced. According to EST sequences.

    This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q9SKB7-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MDSVIIEPDE REALASLVPA HPLPPRKTHS YVEQCEQKPH HPIRKYSLDE
    60 70 80 90 100
    GSRSVTSDSE AVYFDSSDGE FSTEGVAIVD GRTSGERGNG EECGFVTPPS
    110 120 130 140 150
    KPASQGGGND GGREDDIESL PEFIGAGGGL DVFKVPVRAA VNPGRPPCLE
    160 170 180 190 200
    LRPHPLRETQ TGKFLRNIAC TESQLWAGQE NGVRFWNLEE AYEVGCGLGG
    210 220 230 240 250
    QVRRGDEDTA PFHESVPTSP ALCLLVDHGN RLVWTGHKDG KIRAWKMNQP
    260 270 280 290 300
    NTTTADDSKP FKERLSWQAH RGPVNYIVIS SYGDMWSCSD GGVIKIWTLD
    310 320 330 340 350
    SLEKSLVLKL EEKHMAALLV ERSGIDLRSQ VTVNGTCSIS SSDVKFLLVD
    360 370 380 390 400
    TVKAKVWAVQ HLSFSLWDAQ NKELLKVFNI DGQVENRVDM PPTQGQQVED
    410 420 430 440 450
    TKAKFFSAPK KEKSQGFLQR SRHAIMGAAG AVRRAATRSA GAFAEDTRKV
    460 470 480 490 500
    EAIAIAADGS IWTGSMNGVI AQWDGNGSRL REVNHHQQAV LCFCTFGDRI
    510 520 530 540 550
    YVGYSSGYIQ VLDLGGKLIA SWVSHNEPVI KLAAGGGFIF SLATHGGVRG
    560 570 580 590 600
    WYVTSPGPLD SLIRTELSQK EMAYARQDSV KILIGTWNVG EGRASRGALV
    610 620 630 640 650
    SWLGSAVSDV GIVAIGLQEV DMGAGFLAMS TAKETVGVEG SAVGQWWLDA
    660 670 680 690 700
    IGNALDERNT FERMGSRQLA GLLISLWVRK SIRTHVGDLD VAAVPCGFGR
    710 720 730 740 750
    AIGNKGGVGL RIRVYDRIMC FVNCHLAAHL EAVTRRNADF NHIYRSMVFS
    760 770 780 790 800
    KGQSVYTAAA AGASTSAQAL KNNPNTNNST EEEKSHLASA DLVAFFGDFN
    810 820 830 840 850
    YRLFGITYDE ARDFISHRSF DWLREKDQLR QEMNEGKVFQ GMREALITFP
    860 870 880 890 900
    PTYKFEKNKP GLGGYDSGEK KRIPAWCDRV IYRDNQSISY TECSLKCPVV
    910 920 930 940 950
    SSTIMYEACM DVTESDHKPV RCKLHANIAH TDKSVRRQEL GKIVKSNEKL
    960 970 980 990 1000
    RAMFEELKSV PETSVSTNNI LLHSQDTFIF TIRNTSNSSR AIFNIVCKGQ
    1010 1020 1030 1040 1050
    TLVREDGEEP DNHSRGTFGL PRWLEVSPGA GIIKPDASLQ VKVHHEDSHN
    1060 1070 1080 1090 1100
    SEEFIDGIQQ NSLSEESSDK EVTLIIIVQG SCSTRTISHS IKVRHCSSAA
    1110 1120 1130 1140
    KSLSLVHSKT TTMTKNLEGS TRYQTDANRG GSTRHRTDDS TRRG
    Length:1,144
    Mass (Da):125,708
    Last modified:May 1, 2000 - v1
    Checksum:i414BBDDD0DAB7249
    GO

    Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    F4IRT2F4IRT2_ARATH
    Endonuclease/exonuclease/phosphatas...
    5PTase14 At2g31830, F20M17.13, F20M17_13
    1,173Annotation score:
    A0A1P8AZ51A0A1P8AZ51_ARATH
    Endonuclease/exonuclease/phosphatas...
    5PTase14 At2g31830, F20M17.13, F20M17_13
    905Annotation score:

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY761189 mRNA Translation: AAV87316.1
    AY761193 Genomic DNA Translation: AAV87320.1
    AC006533 Genomic DNA Translation: AAD32289.1
    CP002685 Genomic DNA Translation: AEC08590.1
    PIRiF84725
    RefSeqiNP_180742.1, NM_128741.3 [Q9SKB7-1]
    UniGeneiAt.51207

    Genome annotation databases

    EnsemblPlantsiAT2G31830.1; AT2G31830.1; AT2G31830 [Q9SKB7-1]
    GeneIDi817740
    GrameneiAT2G31830.1; AT2G31830.1; AT2G31830 [Q9SKB7-1]
    KEGGiath:AT2G31830

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY761189 mRNA Translation: AAV87316.1
    AY761193 Genomic DNA Translation: AAV87320.1
    AC006533 Genomic DNA Translation: AAD32289.1
    CP002685 Genomic DNA Translation: AEC08590.1
    PIRiF84725
    RefSeqiNP_180742.1, NM_128741.3 [Q9SKB7-1]
    UniGeneiAt.51207

    3D structure databases

    ProteinModelPortaliQ9SKB7
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G31830.2

    PTM databases

    iPTMnetiQ9SKB7

    Proteomic databases

    PaxDbiQ9SKB7
    PRIDEiQ9SKB7

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G31830.1; AT2G31830.1; AT2G31830 [Q9SKB7-1]
    GeneIDi817740
    GrameneiAT2G31830.1; AT2G31830.1; AT2G31830 [Q9SKB7-1]
    KEGGiath:AT2G31830

    Organism-specific databases

    AraportiAT2G31830

    Phylogenomic databases

    eggNOGiKOG0565 Eukaryota
    COG5411 LUCA
    HOGENOMiHOG000242921
    InParanoidiQ9SKB7
    PhylomeDBiQ9SKB7

    Enzyme and pathway databases

    BRENDAi3.1.3.36 399
    ReactomeiR-ATH-1660499 Synthesis of PIPs at the plasma membrane
    R-ATH-1855183 Synthesis of IP2, IP, and Ins in the cytosol
    R-ATH-1855204 Synthesis of IP3 and IP4 in the cytosol

    Miscellaneous databases

    PROiPR:Q9SKB7

    Gene expression databases

    ExpressionAtlasiQ9SKB7 baseline and differential
    GenevisibleiQ9SKB7 AT

    Family and domain databases

    Gene3Di2.130.10.10, 2 hits
    3.60.10.10, 1 hit
    InterProiView protein in InterPro
    IPR036691 Endo/exonu/phosph_ase_sf
    IPR005135 Endo/exonuclease/phosphatase
    IPR000300 IPPc
    IPR015943 WD40/YVTN_repeat-like_dom_sf
    IPR001680 WD40_repeat
    PfamiView protein in Pfam
    PF03372 Exo_endo_phos, 1 hit
    SMARTiView protein in SMART
    SM00128 IPPc, 1 hit
    SM00320 WD40, 4 hits
    SUPFAMiSSF56219 SSF56219, 2 hits
    ProtoNetiSearch...

    Entry informationi

    Entry nameiIP5PE_ARATH
    AccessioniPrimary (citable) accession number: Q9SKB7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: May 1, 2000
    Last modified: April 25, 2018
    This is version 134 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
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