UniProtKB - Q9SJZ6 (MED18_ARATH)
Protein
Mediator of RNA polymerase II transcription subunit 18
Gene
MED18
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (PubMed:22021418, PubMed:24451981). Involved in the regulation of histone H3 lysine tri-methylation (H3K36me3). Associates with the promoter, coding and terminator regions of target genes suggesting its function in transcription initiation, elongation and termination. Multifunctional protein which regulates plant immunity, especially during necrotrophic fungal infection (e.g. B.cinerea and A.brassicicola), flowering time and responses to hormones (e.g. abscisic acid ABA and ethylene) through interactions with distinct transcription factors (PubMed:24451981).2 Publications
GO - Molecular functioni
- transcription coregulator activity Source: UniProtKB
GO - Biological processi
- abscisic acid-activated signaling pathway Source: UniProtKB-KW
- carpel development Source: TAIR
- defense response Source: UniProtKB-KW
- ethylene-activated signaling pathway Source: UniProtKB-KW
- negative regulation of transcription, DNA-templated Source: TAIR
- petal development Source: TAIR
- production of miRNAs involved in gene silencing by miRNA Source: TAIR
- regulation of defense response to fungus Source: UniProtKB
- regulation of DNA-templated transcription, elongation Source: UniProtKB
- regulation of DNA-templated transcription, initiation Source: UniProtKB
- regulation of DNA-templated transcription, termination Source: UniProtKB
- regulation of histone H3-K36 trimethylation Source: UniProtKB
- regulation of photoperiodism, flowering Source: UniProtKB
- regulation of salicylic acid mediated signaling pathway Source: TAIR
- regulation of timing of transition from vegetative to reproductive phase Source: TAIR
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of vernalization response Source: TAIR
- response to abscisic acid Source: UniProtKB
- response to ethylene Source: UniProtKB
- sepal development Source: TAIR
- specification of floral organ number Source: TAIR
- stamen development Source: TAIR
- termination of RNA polymerase II transcription Source: GO_Central
Keywordsi
Molecular function | Developmental protein |
Biological process | Abscisic acid signaling pathway, Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation, Transcription termination |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 181 Publication |
Gene namesi | Name:MED181 Publication Synonyms:MED18_11 Publication Ordered Locus Names:At2g22370Imported ORF Names:F14M13.23Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G22370 |
TAIRi | locus:2041243, AT2G22370 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- core mediator complex Source: GO_Central
- mediator complex Source: UniProtKB
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Deregulated expression of glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5 leading to enhanced susceptibility to fungal infection (e.g. B.cinerea and A.brassicicola). Altered RNA polymerase II occupancy and histone H3 lysine tri-methylation (H3K36me3) of target genes. Insensitivity to abscisic acid (ABA) and to the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). Delayed flowering.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418352 | 1 – 219 | Mediator of RNA polymerase II transcription subunit 18Add BLAST | 219 |
Proteomic databases
PaxDbi | Q9SJZ6 |
PRIDEi | Q9SJZ6 |
ProteomicsDBi | 239052 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9SJZ6, baseline and differential |
Genevisiblei | Q9SJZ6, AT |
Interactioni
Subunit structurei
Component of the Mediator complex (PubMed:17560376).
Interacts with YY1 to suppress disease susceptibility via the repression of genes glutaredoxins GRX480, GRXS13 and thioredoxin TRX-h5. Binds to ABI4 to regulate abscisic acid responses; recruited by ABI4 to ABI5 promoter in the presence of abscisic acid (ABA).
Interacts with SUF4 to regulate flowering time; recruited by SUF4 to FLC promoter (PubMed:24451981).
2 PublicationsBinary interactionsi
Q9SJZ6
With | #Exp. | IntAct |
---|---|---|
MED20A [Q6NPF4] | 3 | EBI-1386265,EBI-1386270 |
Protein-protein interaction databases
BioGRIDi | 2122, 2 interactors |
IntActi | Q9SJZ6, 3 interactors |
STRINGi | 3702.AT2G22370.1 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 203 – 208 | Poly-AlaSequence analysis | 6 |
Sequence similaritiesi
Belongs to the Mediator complex subunit 18 family.Curated
Phylogenomic databases
eggNOGi | KOG3264, Eukaryota |
HOGENOMi | CLU_111349_0_0_1 |
InParanoidi | Q9SJZ6 |
OMAi | PEPAWTV |
OrthoDBi | 1140930at2759 |
PhylomeDBi | Q9SJZ6 |
Family and domain databases
InterProi | View protein in InterPro IPR019095, Mediator_Med18 |
PANTHERi | PTHR13321, PTHR13321, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SJZ6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSMECVVQGI IETQHVEALE ILLQGLCGVQ RERLRVHELC LRSGPNLGVV
60 70 80 90 100
SSEVRLLCDL DQPEPTWTVK HVGGAMRGAG ADQISVLVRN MIESKVSKNA
110 120 130 140 150
LRMFYALGYK LDHELLKVGF AFHFQRTAHI SVSVSSVNKM PKVHAIDEAV
160 170 180 190 200
PVTPGMQIVD VTAPATSENY SEVAAAVSSF CEFLAPLVHL SKPSISTGVV
210
PTAAAAAASL MSDGGGTTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC006592 Genomic DNA Translation: AAD22361.1 CP002685 Genomic DNA Translation: AEC07298.1 BT029378 mRNA Translation: ABK32192.1 |
PIRi | H84611 |
RefSeqi | NP_565534.1, NM_127802.4 |
Genome annotation databases
EnsemblPlantsi | AT2G22370.1; AT2G22370.1; AT2G22370 |
GeneIDi | 816769 |
Gramenei | AT2G22370.1; AT2G22370.1; AT2G22370 |
KEGGi | ath:AT2G22370 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC006592 Genomic DNA Translation: AAD22361.1 CP002685 Genomic DNA Translation: AEC07298.1 BT029378 mRNA Translation: ABK32192.1 |
PIRi | H84611 |
RefSeqi | NP_565534.1, NM_127802.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 2122, 2 interactors |
IntActi | Q9SJZ6, 3 interactors |
STRINGi | 3702.AT2G22370.1 |
Proteomic databases
PaxDbi | Q9SJZ6 |
PRIDEi | Q9SJZ6 |
ProteomicsDBi | 239052 |
Genome annotation databases
EnsemblPlantsi | AT2G22370.1; AT2G22370.1; AT2G22370 |
GeneIDi | 816769 |
Gramenei | AT2G22370.1; AT2G22370.1; AT2G22370 |
KEGGi | ath:AT2G22370 |
Organism-specific databases
Araporti | AT2G22370 |
TAIRi | locus:2041243, AT2G22370 |
Phylogenomic databases
eggNOGi | KOG3264, Eukaryota |
HOGENOMi | CLU_111349_0_0_1 |
InParanoidi | Q9SJZ6 |
OMAi | PEPAWTV |
OrthoDBi | 1140930at2759 |
PhylomeDBi | Q9SJZ6 |
Miscellaneous databases
PROi | PR:Q9SJZ6 |
Gene expression databases
ExpressionAtlasi | Q9SJZ6, baseline and differential |
Genevisiblei | Q9SJZ6, AT |
Family and domain databases
InterProi | View protein in InterPro IPR019095, Mediator_Med18 |
PANTHERi | PTHR13321, PTHR13321, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MED18_ARATH | |
Accessioni | Q9SJZ6Primary (citable) accession number: Q9SJZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2012 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families