UniProtKB - Q9SJY5 (PUMP5_ARATH)
Mitochondrial uncoupling protein 5
PUMP5
Functioni
PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. May be involved in protecting plant cells against oxidative stress damage. Recombinant PUMP5, reconstituted into liposomes, transports a wide range of dicarboxylic acids including malate, oxaloacetate and succinate as well as phosphate, sulfate and thiosulfate. However, it is unknown if these transports are of any biological significance in vivo.
2 PublicationsGO - Molecular functioni
- antiporter activity Source: GO_Central
- dicarboxylic acid transmembrane transporter activity Source: TAIR
- malate transmembrane transporter activity Source: GO_Central
- oxaloacetate transmembrane transporter activity Source: GO_Central
- oxidative phosphorylation uncoupler activity Source: UniProtKB
- succinate transmembrane transporter activity Source: GO_Central
- sulfate transmembrane transporter activity Source: GO_Central
- thiosulfate transmembrane transporter activity Source: GO_Central
GO - Biological processi
- cellular response to hypoxia Source: TAIR
- malate transmembrane transport Source: GO_Central
- mitochondrial transport Source: TAIR
- oxaloacetate transport Source: GO_Central
- phosphate ion transmembrane transport Source: GO_Central
- proton transmembrane transport Source: UniProtKB
- succinate transmembrane transport Source: GO_Central
- sulfate transport Source: GO_Central
- thiosulfate transport Source: GO_Central
Keywordsi
Biological process | Stress response, Transport |
Protein family/group databases
TCDBi | 2.A.29.2.9, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial uncoupling protein 5Short name: AtPUMP5 Alternative name(s): Mitochondrial dicarboxylate carrier 1 |
Gene namesi | Name:PUMP5 Synonyms:DIC1, UCP5 Ordered Locus Names:At2g22500 ORF Names:F14M13.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G22500 |
TAIRi | locus:2041183, AT2G22500 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 6 – 26 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 77 – 97 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Transmembranei | 123 – 143 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 182 – 202 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 223 – 243 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Transmembranei | 280 – 300 | Helical; Name=6Sequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420259 | 1 – 313 | Mitochondrial uncoupling protein 5Add BLAST | 313 |
Proteomic databases
PaxDbi | Q9SJY5 |
ProteomicsDBi | 226268 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
ExpressionAtlasi | Q9SJY5, baseline and differential |
Genevisiblei | Q9SJY5, AT |
Structurei
3D structure databases
AlphaFoldDBi | Q9SJY5 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 4 – 108 | Solcar 1Add BLAST | 105 | |
Repeati | 117 – 208 | Solcar 2Add BLAST | 92 | |
Repeati | 217 – 307 | Solcar 3Add BLAST | 91 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0759, Eukaryota |
HOGENOMi | CLU_015166_14_1_1 |
InParanoidi | Q9SJY5 |
OMAi | MMDTSTM |
OrthoDBi | 892773at2759 |
PhylomeDBi | Q9SJY5 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGLKGFAEGG IASIVAGCST HPLDLIKVRM QLQGESAPIQ TNLRPALAFQ
60 70 80 90 100
TSTTVNAPPL RVGVIGVGSR LIREEGMRAL FSGVSATVLR QTLYSTTRMG
110 120 130 140 150
LYDIIKGEWT DPETKTMPLM KKIGAGAIAG AIGAAVGNPA DVAMVRMQAD
160 170 180 190 200
GRLPLTDRRN YKSVLDAITQ MIRGEGVTSL WRGSSLTINR AMLVTSSQLA
210 220 230 240 250
SYDSVKETIL EKGLLKDGLG THVSASFAAG FVASVASNPV DVIKTRVMNM
260 270 280 290 300
KVVAGVAPPY KGAVDCALKT VKAEGIMSLY KGFIPTVSRQ APFTVVLFVT
310
LEQVKKLFKD YDF
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 130 | G → V in AAK44155 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM236862 mRNA Translation: CAJ86454.1 AC006592 Genomic DNA Translation: AAD22351.1 CP002685 Genomic DNA Translation: AEC07315.1 AF370340 mRNA Translation: AAK44155.1 AF370588 mRNA Translation: AAK43907.1 AY142648 mRNA Translation: AAN13106.1 AK230325 mRNA Translation: BAF02125.1 Frameshift. |
PIRi | D84613 |
RefSeqi | NP_179836.1, NM_127816.3 |
Genome annotation databases
EnsemblPlantsi | AT2G22500.1; AT2G22500.1; AT2G22500 |
GeneIDi | 816783 |
Gramenei | AT2G22500.1; AT2G22500.1; AT2G22500 |
KEGGi | ath:AT2G22500 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM236862 mRNA Translation: CAJ86454.1 AC006592 Genomic DNA Translation: AAD22351.1 CP002685 Genomic DNA Translation: AEC07315.1 AF370340 mRNA Translation: AAK44155.1 AF370588 mRNA Translation: AAK43907.1 AY142648 mRNA Translation: AAN13106.1 AK230325 mRNA Translation: BAF02125.1 Frameshift. |
PIRi | D84613 |
RefSeqi | NP_179836.1, NM_127816.3 |
3D structure databases
AlphaFoldDBi | Q9SJY5 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 3702.AT2G22500.1 |
Protein family/group databases
TCDBi | 2.A.29.2.9, the mitochondrial carrier (mc) family |
Proteomic databases
PaxDbi | Q9SJY5 |
ProteomicsDBi | 226268 |
Genome annotation databases
EnsemblPlantsi | AT2G22500.1; AT2G22500.1; AT2G22500 |
GeneIDi | 816783 |
Gramenei | AT2G22500.1; AT2G22500.1; AT2G22500 |
KEGGi | ath:AT2G22500 |
Organism-specific databases
Araporti | AT2G22500 |
TAIRi | locus:2041183, AT2G22500 |
Phylogenomic databases
eggNOGi | KOG0759, Eukaryota |
HOGENOMi | CLU_015166_14_1_1 |
InParanoidi | Q9SJY5 |
OMAi | MMDTSTM |
OrthoDBi | 892773at2759 |
PhylomeDBi | Q9SJY5 |
Miscellaneous databases
PROi | PR:Q9SJY5 |
Gene expression databases
ExpressionAtlasi | Q9SJY5, baseline and differential |
Genevisiblei | Q9SJY5, AT |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PUMP5_ARATH | |
Accessioni | Q9SJY5Primary (citable) accession number: Q9SJY5 Secondary accession number(s): Q0WL82, Q94K32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2012 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families