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Protein

Triphosphate tunel metalloenzyme 3

Gene

TTM3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of the beta-gamma-phosphoanhydride linkage of triphosphate-containing substrates (inorganic or nucleoside-linked). Catalyzes the hydrolysis of inorganic triphosphate (PPPi), however it does not display significant activity towards long-chain polyphosphates. The existence of PPPi in living cells is still unclear, and PPPase activity might be the ancestral function of CYTH domain. It also has gamma-phosphatase activity on NTP substrates, but no adenylate cyclase or RNA triphosphatase activity.1 Publication

Catalytic activityi

Triphosphate + H2O = diphosphate + phosphate.1 Publication
ATP + H2O = ADP + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+1 PublicationNote: PPPase is active in the presence of Mg2+ ions, while no activity is observed in the presence of manganese ions.1 Publication
  • Mg2+1 Publication, Mn2+1 Publication, Co2+1 PublicationNote: ATPase occurs in the presence of Mg2+ ions as well as Mn2+ and Co2+ ions.1 Publication

Enzyme regulationi

Inhibited by 2.5 mM Ca2+.1 Publication

Kineticsi

  1. KM=1.08 mM for ATP1 Publication
  2. KM=42.9 µM for triphosphate1 Publication

    pH dependencei

    Optimum pH is 9.5 (in the presence of Mg2+. No activity at pH 7.5.1 Publication

    Temperature dependencei

    Optimum temperature is 45.50 degrees Celsius.1 Publication

    GO - Molecular functioni

    • ATPase activity Source: UniProtKB
    • ATP binding Source: UniProtKB-KW
    • triphosphatase activity Source: UniProtKB

    GO - Biological processi

    • root development Source: TAIR

    Keywordsi

    Molecular functionHydrolase
    LigandATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Triphosphate tunel metalloenzyme 3
    Alternative name(s):
    Adenosinetriphosphatase (EC:3.6.1.3)
    Short name:
    ATPase
    Triphosphatase (EC:3.6.1.25)
    Short name:
    PPPase
    Gene namesi
    Name:TTM3
    Ordered Locus Names:At2g11890
    ORF Names:F23M2.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G11890
    TAIRilocus:2045879 AT2G11890

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Delayed root growth and reduced length and number of lateral roots.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi2E → A: Loss of 98.9% of the ATPase activity and 97.3% of the tripolyphosphatase activity. 1 Publication1
    Mutagenesisi52R → A: Loss of 96.7% of the ATPase activity and 98.8% of the tripolyphosphatase activity. 1 Publication1
    Mutagenesisi154E → A: Loss of 98.2% of the ATPase activity and 72.7% of the tripolyphosphatase activity. 1 Publication1
    Mutagenesisi171E → A: Total loss of ATPase activity and 92.5% of the tripolyphosphatase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004267371 – 210Triphosphate tunel metalloenzyme 3Add BLAST210

    Proteomic databases

    PaxDbiQ9SIY3
    PRIDEiQ9SIY3

    PTM databases

    iPTMnetiQ9SIY3

    Expressioni

    Tissue specificityi

    Expressed only in the proximal meristematic zone of the root.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9SIY3 baseline and differential
    GenevisibleiQ9SIY3 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G11890.1

    Structurei

    Secondary structure

    1210
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi1 – 9Combined sources9
    Helixi12 – 22Combined sources11
    Helixi23 – 25Combined sources3
    Beta strandi26 – 38Combined sources13
    Helixi43 – 46Combined sources4
    Beta strandi49 – 58Combined sources10
    Beta strandi63 – 65Combined sources3
    Beta strandi70 – 82Combined sources13
    Beta strandi85 – 95Combined sources11
    Helixi97 – 105Combined sources9
    Helixi107 – 112Combined sources6
    Helixi116 – 125Combined sources10
    Beta strandi134 – 148Combined sources15
    Beta strandi151 – 160Combined sources10
    Beta strandi163 – 171Combined sources9
    Helixi175 – 188Combined sources14
    Beta strandi193 – 195Combined sources3
    Helixi200 – 206Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3V85X-ray1.90A1-210[»]
    5A5YX-ray1.92A/B1-210[»]
    5A66X-ray2.05A/B1-210[»]
    5A67X-ray1.30A1-210[»]
    5A68X-ray1.67A1-210[»]
    ProteinModelPortaliQ9SIY3
    SMRiQ9SIY3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini1 – 208CYTHPROSITE-ProRule annotationAdd BLAST208

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi89 – 192Glu-richAdd BLAST104

    Phylogenomic databases

    eggNOGiENOG410IW2P Eukaryota
    ENOG4111IHI LUCA
    HOGENOMiHOG000242707
    InParanoidiQ9SIY3
    OMAiRNVYEWR
    OrthoDBiEOG09360MS1
    PhylomeDBiQ9SIY3

    Family and domain databases

    InterProiView protein in InterPro
    IPR033469 CYTH-like_dom_sf
    IPR023577 CYTH_domain
    IPR033245 TTM3
    PANTHERiPTHR34948:SF1 PTHR34948:SF1, 1 hit
    PfamiView protein in Pfam
    PF01928 CYTH, 1 hit
    SMARTiView protein in SMART
    SM01118 CYTH, 1 hit
    SUPFAMiSSF55154 SSF55154, 1 hit
    PROSITEiView protein in PROSITE
    PS51707 CYTH, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9SIY3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MEVEVKLRLL TAAAHLRLTT LLTPYHLKTL HQRNTFFDTP KNDLSLRRAV
    60 70 80 90 100
    LRLRFLQNAA VSAASPSPPR CIVSLKAKPT LANGISRVEE DEEEIEYWIG
    110 120 130 140 150
    KECVESPAKL SDIGSRVLKR VKEEYGFNDF LGFVCLGGFE NVRNVYEWRG
    160 170 180 190 200
    VKLEVDETKY DFGNCYEIEC ETEEPERVKT MIEEFLTEEK IEFSNSDMTK
    210
    FAVFRSGKLP
    Length:210
    Mass (Da):24,163
    Last modified:May 1, 2000 - v1
    Checksum:i56E70B8055143A33
    GO
    Isoform 2 (identifier: Q9SIY3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         100-136: Missing.

    Show »
    Length:173
    Mass (Da):20,046
    Checksum:iBCD623F6A413147B
    GO

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_053969100 – 136Missing in isoform 2. 1 PublicationAdd BLAST37

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC007045 Genomic DNA Translation: AAD22501.1
    CP002685 Genomic DNA Translation: AEC06186.1
    CP002685 Genomic DNA Translation: AEC06187.1
    AY045689 mRNA Translation: AAK74047.1
    AY055788 mRNA Translation: AAL06955.1
    AK316974 mRNA Translation: BAH19671.1
    AY088524 mRNA Translation: AAM66058.1
    AK229646 mRNA Translation: BAF01490.1
    PIRiE84499
    RefSeqiNP_001031347.1, NM_001036270.1 [Q9SIY3-2]
    NP_565353.1, NM_126867.2 [Q9SIY3-1]
    UniGeneiAt.14286

    Genome annotation databases

    EnsemblPlantsiAT2G11890.1; AT2G11890.1; AT2G11890 [Q9SIY3-1]
    AT2G11890.2; AT2G11890.2; AT2G11890 [Q9SIY3-2]
    GeneIDi815664
    GrameneiAT2G11890.1; AT2G11890.1; AT2G11890 [Q9SIY3-1]
    AT2G11890.2; AT2G11890.2; AT2G11890 [Q9SIY3-2]
    KEGGiath:AT2G11890

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiTTM3_ARATH
    AccessioniPrimary (citable) accession number: Q9SIY3
    Secondary accession number(s): B9DG04
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: May 1, 2000
    Last modified: April 25, 2018
    This is version 114 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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