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Protein

Casparian strip membrane protein 1

Gene

CASP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion.1 Publication

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: GO_Central
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • cell-cell junction assembly Source: UniProtKB
  • cell wall modification Source: UniProtKB

Keywordsi

Biological processCell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Casparian strip membrane protein 1
Short name:
AtCASP1
Gene namesi
Name:CASP1
Ordered Locus Names:At2g36100
ORF Names:F9C22.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G36100
TAIRilocus:2053514 AT2G36100

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 43CytoplasmicSequence analysisAdd BLAST42
Transmembranei44 – 64HelicalSequence analysisAdd BLAST21
Topological domaini65 – 95ExtracellularSequence analysisAdd BLAST31
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 127CytoplasmicSequence analysisAdd BLAST11
Transmembranei128 – 148HelicalSequence analysisAdd BLAST21
Topological domaini149 – 180ExtracellularSequence analysisAdd BLAST32
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 206CytoplasmicSequence analysis5

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disorganised deposition of Casparian strips.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi72 – 80Missing : Normal subcellular localization. 1 Publication9
Mutagenesisi73 – 79Missing : Normal subcellular localization. 1 Publication7
Mutagenesisi74 – 78Missing : Normal subcellular localization. 1 Publication5
Mutagenesisi134D → H: Not detectable probably due to an impaired folding leading to instability. 1 Publication1
Mutagenesisi158 – 175Missing : Normal subcellular localization. 1 PublicationAdd BLAST18
Mutagenesisi158G → S: Abnormal subcellular localization. 1 Publication1
Mutagenesisi164W → G: Abnormal subcellular localization. 1 Publication1
Mutagenesisi168C → S: Abnormal subcellular localization. 1 Publication1
Mutagenesisi170Q → E: Normal subcellular localization. 1 Publication1
Mutagenesisi174F → V: Abnormal subcellular localization. 1 Publication1
Mutagenesisi175C → S: Abnormal subcellular localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003086592 – 206Casparian strip membrane protein 1Add BLAST205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Glycosylationi159N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PaxDbiQ9SIH4

PTM databases

iPTMnetiQ9SIH4

Expressioni

Gene expression databases

ExpressionAtlasiQ9SIH4 baseline and differential
GenevisibleiQ9SIH4 AT

Interactioni

Subunit structurei

Homodimer and heterodimers with other CASP proteins. Interacts with CASP2, CASP3, CASP4 and CASP5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CASP3Q9ZQI22EBI-4451446,EBI-15927653

GO - Molecular functioni

Protein-protein interaction databases

BioGridi3527, 4 interactors
DIPiDIP-59177N
IntActiQ9SIH4, 8 interactors
STRINGi3702.AT2G36100.1

Structurei

3D structure databases

ProteinModelPortaliQ9SIH4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYVB Eukaryota
ENOG410YJEC LUCA
HOGENOMiHOG000237690
InParanoidiQ9SIH4
OMAiAFQYFVI
OrthoDBiEOG09360W0R
PhylomeDBiQ9SIH4

Family and domain databases

InterProiView protein in InterPro
IPR006459 CASP/CASPL
IPR006702 CASP_dom
PfamiView protein in Pfam
PF04535 DUF588, 1 hit
TIGRFAMsiTIGR01569 A_tha_TIGR01569, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9SIH4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKESTTIDV GEPSTVTKSS SHVVKDAKKK GFVAVASRGG AKRGLAIFDF
60 70 80 90 100
LLRLAAIAVT IGAASVMYTA EETLPFFTQF LQFQAGYDDL PAFQYFVIAV
110 120 130 140 150
AVVASYLVLS LPFSIVSIVR PHAVAPRLIL LICDTLVVTL NTSAAAAAAS
160 170 180 190 200
ITYLAHNGNQ STNWLPICQQ FGDFCQNVST AVVADSIAIL FFIVLIIISA

IALKRH
Length:206
Mass (Da):21,970
Last modified:May 1, 2000 - v1
Checksum:iE0BCBE459A96C771
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007135 Genomic DNA Translation: AAD26967.1
CP002685 Genomic DNA Translation: AEC09206.1
BT003113 mRNA Translation: AAO24545.1
AK228127 mRNA Translation: BAF00084.1
PIRiH84776
RefSeqiNP_181154.1, NM_129169.3
UniGeneiAt.42995

Genome annotation databases

EnsemblPlantsiAT2G36100.1; AT2G36100.1; AT2G36100
GeneIDi818183
GrameneiAT2G36100.1; AT2G36100.1; AT2G36100
KEGGiath:AT2G36100

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007135 Genomic DNA Translation: AAD26967.1
CP002685 Genomic DNA Translation: AEC09206.1
BT003113 mRNA Translation: AAO24545.1
AK228127 mRNA Translation: BAF00084.1
PIRiH84776
RefSeqiNP_181154.1, NM_129169.3
UniGeneiAt.42995

3D structure databases

ProteinModelPortaliQ9SIH4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3527, 4 interactors
DIPiDIP-59177N
IntActiQ9SIH4, 8 interactors
STRINGi3702.AT2G36100.1

PTM databases

iPTMnetiQ9SIH4

Proteomic databases

PaxDbiQ9SIH4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36100.1; AT2G36100.1; AT2G36100
GeneIDi818183
GrameneiAT2G36100.1; AT2G36100.1; AT2G36100
KEGGiath:AT2G36100

Organism-specific databases

AraportiAT2G36100
TAIRilocus:2053514 AT2G36100

Phylogenomic databases

eggNOGiENOG410IYVB Eukaryota
ENOG410YJEC LUCA
HOGENOMiHOG000237690
InParanoidiQ9SIH4
OMAiAFQYFVI
OrthoDBiEOG09360W0R
PhylomeDBiQ9SIH4

Miscellaneous databases

PROiPR:Q9SIH4

Gene expression databases

ExpressionAtlasiQ9SIH4 baseline and differential
GenevisibleiQ9SIH4 AT

Family and domain databases

InterProiView protein in InterPro
IPR006459 CASP/CASPL
IPR006702 CASP_dom
PfamiView protein in Pfam
PF04535 DUF588, 1 hit
TIGRFAMsiTIGR01569 A_tha_TIGR01569, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCASP1_ARATH
AccessioniPrimary (citable) accession number: Q9SIH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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