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Protein

Heat shock protein 90-5, chloroplastic

Gene

HSP90-5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone required for chloroplast biogenesis (PubMed:12943545, PubMed:25216779). Essential for chloroplast biogenesis and maintenance, and thus for embryogenesis (PubMed:23875936, PubMed:23382192). May be involved in the disassembly of VIPP1 for thylakoid membrane formation and/or maintenance (PubMed:23875936). Cooperates with TIC components and other molecular chaperones to drive transport of preproteins into chloroplasts and functions in the chloroplast stroma to facilitate membrane translocation during protein import into the organelle (PubMed:23382192).4 Publications

Miscellaneous

Plants over-expressing HSP90.7 show albino and stunted leaves.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106ATPBy similarity1
Binding sitei110ATPBy similarity1
Binding sitei152ATPBy similarity1
Binding sitei157ATPBy similarity1
Binding sitei251ATPBy similarity1
Binding sitei441ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 173ATPBy similarity2
Nucleotide bindingi196 – 201ATPBy similarity6

GO - Molecular functioni

  • ATP binding Source: TAIR
  • protein homodimerization activity Source: UniProtKB
  • unfolded protein binding Source: InterPro

GO - Biological processi

  • de-etiolation Source: TAIR
  • protein folding Source: InterPro
  • protein import into chloroplast stroma Source: TAIR
  • response to chlorate Source: TAIR
  • response to heat Source: TAIR
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Stress response, Transport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 90-5, chloroplasticCurated
Short name:
AtHSP90.5Curated
Short name:
AtHsp90-51 Publication
Alternative name(s):
Heat shock protein 88-1Curated
Short name:
Hsp88-11 Publication
Hsp90C1 Publication
Protein EMBRYO DEFECTIVE 19561 Publication
Protein chlorate-resistance 881 Publication
Gene namesi
Name:HSP90-51 Publication
Synonyms:CR881 Publication, EMB19561 Publication, HSP88-11 Publication
Ordered Locus Names:At2g04030Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G04030
TAIRilocus:2049651 AT2G04030

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Embyronic lethality due to embryo development arrest at the heart stage.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi646G → R in cr88; delay in greening of young rosette leaves. Reduced nitrate reductase activity in response to nitrate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 60ChloroplastSequence analysisAdd BLAST60
ChainiPRO_000043401861 – 780Heat shock protein 90-5, chloroplasticSequence analysisAdd BLAST720

Proteomic databases

PaxDbiQ9SIF2
PRIDEiQ9SIF2
ProMEXiQ9SIF2

PTM databases

SwissPalmiQ9SIF2

Expressioni

Tissue specificityi

Expressed in roots, cotyledons, young leaves, mature leaves, stems, flowers, petals and siliques.1 Publication

Developmental stagei

During embryogenesis, highly expressed at 4 days post anthesis.1 Publication

Inductioni

By heat shock and light.1 Publication

Gene expression databases

ExpressionAtlasiQ9SIF2 baseline and differential
GenevisibleiQ9SIF2 AT

Interactioni

Subunit structurei

Homodimer (PubMed:25216779). Interacts with VIPP1 (PubMed:23875936). Interacts with P23-1 (PubMed:20493581).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9SIF2, 2 interactors
STRINGi3702.AT2G04030.1

Structurei

3D structure databases

ProteinModelPortaliQ9SIF2
SMRiQ9SIF2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi313 – 316Poly-LysSequence analysis4
Compositional biasi745 – 749Poly-GluSequence analysis5

Sequence similaritiesi

Belongs to the heat shock protein 90 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0019 Eukaryota
COG0326 LUCA
HOGENOMiHOG000031988
InParanoidiQ9SIF2
KOiK09487
OMAiNTQKAIW
OrthoDBiEOG093603ZM
PhylomeDBiQ9SIF2

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9SIF2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPALSRSLY TSPLTSVPIT PVSSRLSHLR SSFLPHGGAL RTGVSCSWNL
60 70 80 90 100
EKRCNRFAVK CDAAVAEKET TEEGSGEKFE YQAEVSRLLD LIVHSLYSHK
110 120 130 140 150
EVFLRELVSN ASDALDKLRF LSVTEPSLLG DGGDLEIRIK PDPDNGTITI
160 170 180 190 200
TDTGIGMTKE ELIDCLGTIA QSGTSKFLKA LKENKDLGAD NGLIGQFGVG
210 220 230 240 250
FYSAFLVAEK VVVSTKSPKS DKQYVWESVA DSSSYLIREE TDPDNILRRG
260 270 280 290 300
TQITLYLRED DKYEFAESTR IKNLVKNYSQ FVGFPIYTWQ EKSRTIEVEE
310 320 330 340 350
DEPVKEGEEG EPKKKKTTKT EKYWDWELAN ETKPLWMRNS KEVEKGEYNE
360 370 380 390 400
FYKKAFNEFL DPLAHTHFTT EGEVEFRSIL YIPGMGPLNN EDVTNPKTKN
410 420 430 440 450
IRLYVKRVFI SDDFDGELFP RYLSFVKGVV DSDDLPLNVS REILQESRIV
460 470 480 490 500
RIMRKRLIRK TFDMIQEISE SENKEDYKKF WENFGRFLKL GCIEDTGNHK
510 520 530 540 550
RITPLLRFFS SKNEEELTSL DDYIENMGEN QKAIYYLATD SLKSAKSAPF
560 570 580 590 600
LEKLIQKDIE VLYLVEPIDE VAIQNLQTYK EKKFVDISKE DLELGDEDEV
610 620 630 640 650
KDREAKQEFN LLCDWIKQQL GDKVAKVQVS NRLSSSPCVL VSGKFGWSAN
660 670 680 690 700
MERLMKAQAL GDTSSLEFMR GRRILEINPD HPIIKDLNAA CKNAPESTEA
710 720 730 740 750
TRVVDLLYDT AIISSGFTPD SPAELGNKIY EMMAMAVGGR WGRVEEEEES
760 770 780
STVNEGDDKS GETEVVEPSE VRAESDPWQD
Length:780
Mass (Da):88,663
Last modified:May 1, 2000 - v1
Checksum:i6838772716E75924
GO
Isoform 2 (identifier: Q9SIF2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     476-478: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:777
Mass (Da):88,257
Checksum:i3FA8AF15B1F26DAA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224Y → N in AAM19795 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057880476 – 478Missing in isoform 2. 3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007167 Genomic DNA Translation: AAD32922.1
CP002685 Genomic DNA Translation: AEC05778.1
CP002685 Genomic DNA Translation: AEC05779.1
AF436826 mRNA Translation: AAL32008.1
AY053403 mRNA Translation: AAK96633.1
AY094422 mRNA Translation: AAM19795.1
BT002234 mRNA Translation: AAN72245.1
AK229707 mRNA Translation: BAF01546.1
AK229761 mRNA Translation: BAF01597.1
PIRiH84453
RefSeqiNP_178487.1, NM_126439.4 [Q9SIF2-1]
NP_849932.1, NM_179601.1 [Q9SIF2-2]
UniGeneiAt.1835

Genome annotation databases

EnsemblPlantsiAT2G04030.1; AT2G04030.1; AT2G04030 [Q9SIF2-1]
AT2G04030.2; AT2G04030.2; AT2G04030 [Q9SIF2-2]
GeneIDi814930
GrameneiAT2G04030.1; AT2G04030.1; AT2G04030 [Q9SIF2-1]
AT2G04030.2; AT2G04030.2; AT2G04030 [Q9SIF2-2]
KEGGiath:AT2G04030

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007167 Genomic DNA Translation: AAD32922.1
CP002685 Genomic DNA Translation: AEC05778.1
CP002685 Genomic DNA Translation: AEC05779.1
AF436826 mRNA Translation: AAL32008.1
AY053403 mRNA Translation: AAK96633.1
AY094422 mRNA Translation: AAM19795.1
BT002234 mRNA Translation: AAN72245.1
AK229707 mRNA Translation: BAF01546.1
AK229761 mRNA Translation: BAF01597.1
PIRiH84453
RefSeqiNP_178487.1, NM_126439.4 [Q9SIF2-1]
NP_849932.1, NM_179601.1 [Q9SIF2-2]
UniGeneiAt.1835

3D structure databases

ProteinModelPortaliQ9SIF2
SMRiQ9SIF2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9SIF2, 2 interactors
STRINGi3702.AT2G04030.1

PTM databases

SwissPalmiQ9SIF2

Proteomic databases

PaxDbiQ9SIF2
PRIDEiQ9SIF2
ProMEXiQ9SIF2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G04030.1; AT2G04030.1; AT2G04030 [Q9SIF2-1]
AT2G04030.2; AT2G04030.2; AT2G04030 [Q9SIF2-2]
GeneIDi814930
GrameneiAT2G04030.1; AT2G04030.1; AT2G04030 [Q9SIF2-1]
AT2G04030.2; AT2G04030.2; AT2G04030 [Q9SIF2-2]
KEGGiath:AT2G04030

Organism-specific databases

AraportiAT2G04030
TAIRilocus:2049651 AT2G04030

Phylogenomic databases

eggNOGiKOG0019 Eukaryota
COG0326 LUCA
HOGENOMiHOG000031988
InParanoidiQ9SIF2
KOiK09487
OMAiNTQKAIW
OrthoDBiEOG093603ZM
PhylomeDBiQ9SIF2

Miscellaneous databases

PROiPR:Q9SIF2

Gene expression databases

ExpressionAtlasiQ9SIF2 baseline and differential
GenevisibleiQ9SIF2 AT

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di1.20.120.790, 1 hit
3.30.565.10, 1 hit
HAMAPiMF_00505 HSP90, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR019805 Heat_shock_protein_90_CS
IPR037196 HSP90_C
IPR001404 Hsp90_fam
IPR020575 Hsp90_N
IPR020568 Ribosomal_S5_D2-typ_fold
PANTHERiPTHR11528 PTHR11528, 1 hit
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00183 HSP90, 1 hit
PIRSFiPIRSF002583 Hsp90, 1 hit
PRINTSiPR00775 HEATSHOCK90
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SUPFAMiSSF110942 SSF110942, 1 hit
SSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS00298 HSP90, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHS905_ARATH
AccessioniPrimary (citable) accession number: Q9SIF2
Secondary accession number(s): F4IU89
, Q0WMQ3, Q0WMV4, Q8LPS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: May 1, 2000
Last modified: October 10, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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