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UniProtKB - Q9SIB9 (ACO3M_ARATH)
Protein
Aconitate hydratase 3, mitochondrial
Gene
ACO3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Contributes to oxidative stress tolerance (PubMed:17013749).
Modulates cytosolic citrate metabolism during lipid mobilization. Required during seedling growth (PubMed:25061985).
2 PublicationsCatalytic activityi
- EC:4.2.1.3By similarity
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 182 | SubstrateBy similarity | 1 | |
Metal bindingi | 533 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 599 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 602 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Binding sitei | 632 | SubstrateBy similarity | 1 | |
Binding sitei | 637 | SubstrateBy similarity | 1 | |
Binding sitei | 795 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: GO_Central
- aconitate hydratase activity Source: UniProtKB
- ATP binding Source: TAIR
- citrate dehydratase activity Source: UniProtKB-EC
- copper ion binding Source: TAIR
GO - Biological processi
- citrate metabolic process Source: UniProtKB
- glyoxylate cycle Source: UniProtKB-KW
- isocitrate metabolic process Source: TAIR
- response to abscisic acid Source: TAIR
- response to oxidative stress Source: TAIR
- seedling development Source: UniProtKB
- tricarboxylic acid cycle Source: GO_Central
Keywordsi
Molecular function | Lyase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BRENDAi | 4.2.1.3, 399 |
UniPathwayi | UPA00223;UER00718 |
Names & Taxonomyi
Protein namesi | Recommended name: Aconitate hydratase 3, mitochondrial1 Publication (EC:4.2.1.3By similarity)Short name: Aconitase 31 Publication Short name: mACO11 Publication Alternative name(s): Citrate hydro-lyase 31 Publication |
Gene namesi | Name:ACO31 Publication Ordered Locus Names:At2g05710Imported ORF Names:T3P4.5Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G05710 |
TAIRi | locus:2063354, AT2G05710 |
Subcellular locationi
Mitochondrion
- Mitochondrion 2 Publications
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Note: Cytosolic localization in 3-day-old seedlings, but mitochondrial in 10-day-old plantlets.1 Publication
Cell Wall
- plant-type cell wall Source: TAIR
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MitochondrionPathology & Biotechi
Disruption phenotypei
Reduced cytosolic and mitochondrial aconitase (ACO) activities by 25 and 55 precent, respectively (PubMed:17013749, PubMed:17437406). Increased tolerance to oxidative stress mediated by paraquat, a superoxide-generating agent (PubMed:17013749). Delayed early seedling growth, altered assimilation of acetate feeding and elevated citrate and malate levels (PubMed:25061985).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 78 | MitochondrionSequence analysisAdd BLAST | 78 | |
ChainiPRO_0000259921 | 79 – 990 | Aconitate hydratase 3, mitochondrialAdd BLAST | 912 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Phosphorylated at Ser-91 in the cytoplasm; this phosphorylation requires the presence of B'GAMMA.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9SIB9 |
PRIDEi | Q9SIB9 |
ProteomicsDBi | 243283 |
PTM databases
iPTMneti | Q9SIB9 |
MetOSitei | Q9SIB9 |
Expressioni
Tissue specificityi
Major aconitase isoenzyme in young seedlings (PubMed:25061985). Expressed in roots, leaves, stems and flowers, and, at low levels, in seeds (PubMed:17437406).2 Publications
Inductioni
Transiently induced during germination.1 Publication
Gene expression databases
ExpressionAtlasi | Q9SIB9, baseline and differential |
Genevisiblei | Q9SIB9, AT |
Interactioni
Subunit structurei
Monomer (By similarity).
Interacts with B'GAMMA in the cytosol (PubMed:25307043).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 519, 23 interactors |
IntActi | Q9SIB9, 1 interactor |
STRINGi | 3702.AT2G05710.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 301 – 303 | Substrate bindingBy similarity | 3 | |
Regioni | 876 – 877 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the aconitase/IPM isomerase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0452, Eukaryota |
HOGENOMi | CLU_013476_2_1_1 |
InParanoidi | Q9SIB9 |
OMAi | ACQRYNM |
OrthoDBi | 190960at2759 |
PhylomeDBi | Q9SIB9 |
Family and domain databases
CDDi | cd01580, AcnA_IRP_Swivel, 1 hit |
Gene3Di | 3.20.19.10, 1 hit 3.30.499.10, 2 hits |
InterProi | View protein in InterPro IPR044137, AcnA_IRP_Swivel IPR015931, Acnase/IPM_dHydase_lsu_aba_1/3 IPR001030, Acoase/IPM_deHydtase_lsu_aba IPR015928, Aconitase/3IPM_dehydase_swvl IPR006249, Aconitase/IRP2 IPR018136, Aconitase_4Fe-4S_BS IPR036008, Aconitase_4Fe-4S_dom IPR000573, AconitaseA/IPMdHydase_ssu_swvl |
PANTHERi | PTHR11670, PTHR11670, 1 hit |
Pfami | View protein in Pfam PF00330, Aconitase, 1 hit PF00694, Aconitase_C, 1 hit |
PRINTSi | PR00415, ACONITASE |
SUPFAMi | SSF53732, SSF53732, 1 hit |
TIGRFAMsi | TIGR01341, aconitase_1, 1 hit |
PROSITEi | View protein in PROSITE PS00450, ACONITASE_1, 1 hit PS01244, ACONITASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9SIB9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYLTASSSAS SSIIRAASSR SSSLFSFRSV LSPSVSSTSP SSLLARRSFG
60 70 80 90 100
TISPAFRRWS HSFHSKPSPF RFTSQIRAVS PVLDRLQRTF SSMASEHPFK
110 120 130 140 150
GIFTTLPKPG GGEFGKFYSL PALNDPRVDK LPYSIRILLE SAIRNCDNFQ
160 170 180 190 200
VTKEDVEKII DWEKTSPKQV EIPFKPARVL LQDFTGVPAV VDLACMRDAM
210 220 230 240 250
NKLGSDSNKI NPLVPVDLVI DHSVQVDVAR SENAVQANME LEFQRNKERF
260 270 280 290 300
AFLKWGSTAF QNMLVVPPGS GIVHQVNLEY LGRVVFNTKG LLYPDSVVGT
310 320 330 340 350
DSHTTMIDGL GVAGWGVGGI EAEATMLGQP MSMVLPGVVG FKLAGKMRNG
360 370 380 390 400
VTATDLVLTV TQMLRKHGVV GKFVEFYGNG MSGLSLADRA TIANMSPEYG
410 420 430 440 450
ATMGFFPVDH VTLQYLKLTG RSDETVAMIE AYLRANNMFV DYNEPQQDRV
460 470 480 490 500
YSSYLELNLD DVEPCISGPK RPHDRVTLKE MKADWHSCLD SKVGFKGFAI
510 520 530 540 550
PKEAQEKVVN FSFDGQPAEL KHGSVVIAAI TSCTNTSNPS VMLGAGLVAK
560 570 580 590 600
KACDLGLQVK PWIKTSLAPG SGVVTKYLLK SGLQEYLNEQ GFNIVGYGCT
610 620 630 640 650
TCIGNSGEIN ESVGAAITEN DIVAAAVLSG NRNFEGRVHP LTRANYLASP
660 670 680 690 700
PLVVAYALAG TVNIDFETEP IGKGKNGKDV FLRDIWPTTE EIAEVVQSSV
710 720 730 740 750
LPDMFRATYE SITKGNPMWN KLSVPENTLY SWDPNSTYIH EPPYFKDMTM
760 770 780 790 800
DPPGPHNVKD AYCLLNFGDS ITTDHISPAG NIQKDSPAAK FLMERGVDRK
810 820 830 840 850
DFNSYGSRRG NDEIMARGTF ANIRIVNKLM NGEVGPKTVH IPSGEKLSVF
860 870 880 890 900
DAAMRYKSSG EDTIILAGAE YGSGSSRDWA AKGPMLQGVK AVIAKSFERI
910 920 930 940 950
HRSNLVGMGI IPLCFKSGED ADTLGLTGHE RYTIHLPTDI SEIRPGQDVT
960 970 980 990
VTTDNGKSFT CTVRFDTEVE LAYFNHGGIL PYVIRNLSKQ
Sequence cautioni
The sequence AAD25640 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007170 Genomic DNA Translation: AAD25640.1 Different initiation. CP002685 Genomic DNA Translation: AEC05964.1 AY136414 mRNA Translation: AAM97080.1 BT008809 mRNA Translation: AAP68248.1 |
PIRi | B84471 |
RefSeqi | NP_178634.2, NM_126589.3 |
Genome annotation databases
EnsemblPlantsi | AT2G05710.1; AT2G05710.1; AT2G05710 |
GeneIDi | 815120 |
Gramenei | AT2G05710.1; AT2G05710.1; AT2G05710 |
KEGGi | ath:AT2G05710 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007170 Genomic DNA Translation: AAD25640.1 Different initiation. CP002685 Genomic DNA Translation: AEC05964.1 AY136414 mRNA Translation: AAM97080.1 BT008809 mRNA Translation: AAP68248.1 |
PIRi | B84471 |
RefSeqi | NP_178634.2, NM_126589.3 |
3D structure databases
AlphaFoldDBi | Q9SIB9 |
SMRi | Q9SIB9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 519, 23 interactors |
IntActi | Q9SIB9, 1 interactor |
STRINGi | 3702.AT2G05710.1 |
PTM databases
iPTMneti | Q9SIB9 |
MetOSitei | Q9SIB9 |
Proteomic databases
PaxDbi | Q9SIB9 |
PRIDEi | Q9SIB9 |
ProteomicsDBi | 243283 |
Genome annotation databases
EnsemblPlantsi | AT2G05710.1; AT2G05710.1; AT2G05710 |
GeneIDi | 815120 |
Gramenei | AT2G05710.1; AT2G05710.1; AT2G05710 |
KEGGi | ath:AT2G05710 |
Organism-specific databases
Araporti | AT2G05710 |
TAIRi | locus:2063354, AT2G05710 |
Phylogenomic databases
eggNOGi | KOG0452, Eukaryota |
HOGENOMi | CLU_013476_2_1_1 |
InParanoidi | Q9SIB9 |
OMAi | ACQRYNM |
OrthoDBi | 190960at2759 |
PhylomeDBi | Q9SIB9 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER00718 |
BRENDAi | 4.2.1.3, 399 |
Miscellaneous databases
PROi | PR:Q9SIB9 |
Gene expression databases
ExpressionAtlasi | Q9SIB9, baseline and differential |
Genevisiblei | Q9SIB9, AT |
Family and domain databases
CDDi | cd01580, AcnA_IRP_Swivel, 1 hit |
Gene3Di | 3.20.19.10, 1 hit 3.30.499.10, 2 hits |
InterProi | View protein in InterPro IPR044137, AcnA_IRP_Swivel IPR015931, Acnase/IPM_dHydase_lsu_aba_1/3 IPR001030, Acoase/IPM_deHydtase_lsu_aba IPR015928, Aconitase/3IPM_dehydase_swvl IPR006249, Aconitase/IRP2 IPR018136, Aconitase_4Fe-4S_BS IPR036008, Aconitase_4Fe-4S_dom IPR000573, AconitaseA/IPMdHydase_ssu_swvl |
PANTHERi | PTHR11670, PTHR11670, 1 hit |
Pfami | View protein in Pfam PF00330, Aconitase, 1 hit PF00694, Aconitase_C, 1 hit |
PRINTSi | PR00415, ACONITASE |
SUPFAMi | SSF53732, SSF53732, 1 hit |
TIGRFAMsi | TIGR01341, aconitase_1, 1 hit |
PROSITEi | View protein in PROSITE PS00450, ACONITASE_1, 1 hit PS01244, ACONITASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACO3M_ARATH | |
Accessioni | Q9SIB9Primary (citable) accession number: Q9SIB9 Secondary accession number(s): Q8L784 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | November 28, 2006 | |
Last modified: | May 25, 2022 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families