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Protein

Polyprenol reductase 2

Gene

PPRD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Involved in the regulation of plant growth and reproductive processes.1 Publication

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • lipid metabolic process Source: InterPro
  • protein N-linked glycosylation Source: TAIR

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

ReactomeiR-ATH-193048 Androgen biosynthesis
R-ATH-446199 Synthesis of Dolichyl-phosphate
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase 21 Publication (EC:1.3.1.941 Publication)
Gene namesi
Name:PPRD21 Publication
Ordered Locus Names:At2g16530Imported
ORF Names:F1P15.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G16530
TAIRilocus:2045126 AT2G16530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Transmembranei223 – 243HelicalSequence analysisAdd BLAST21
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Male sterility, when homozygous. Deformed and non-viable pollen grains.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003986561 – 343Polyprenol reductase 2Add BLAST343

Proteomic databases

PaxDbiQ9SI62

PTM databases

iPTMnetiQ9SI62

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ9SI62 baseline and differential
GenevisibleiQ9SI62 AT

Interactioni

Protein-protein interaction databases

BioGridi1510, 1 interactor
IntActiQ9SI62, 1 interactor
STRINGi3702.AT2G16530.1

Structurei

3D structure databases

ProteinModelPortaliQ9SI62
SMRiQ9SI62
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1640 Eukaryota
ENOG4111JQM LUCA
HOGENOMiHOG000240963
InParanoidiQ9SI62
KOiK12345
OMAiTSAPEFN
OrthoDBiEOG09360J9J
PhylomeDBiQ9SI62

Family and domain databases

InterProiView protein in InterPro
IPR001104 3-oxo-5_a-steroid_4-DH_C
PfamiView protein in Pfam
PF02544 Steroid_dh, 1 hit
PROSITEiView protein in PROSITE
PS50244 S5A_REDUCTASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9SI62-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVELEIVWLV RGAWITVWIV SILPLVIASI PTSKLNSFRE LVLSFAGRGK
60 70 80 90 100
ILHPSSQKFT IPQKCFAHFY VIGVVWTTLL LAATWMYACK MAPLSSEEFQ
110 120 130 140 150
LSDIASRLAG GSDVFSVHKS NMTPVEHRFK VWRAVFLLLL MEIHVLRRLI
160 170 180 190 200
ESFYVFKYSP SARMHILGYF AGLFFYVTAP LSLCSNIAPE VAGFVGNQVA
210 220 230 240 250
EFIANGKSHT SAPEFNLLSS ISPLMKLGSL QWIGGAIFLW GWIHQRRCHA
260 270 280 290 300
ILGSLRENPS QAKEYIIPYG DWFGMVSSPH FLAEIVLYAG LLIASGGTDI
310 320 330 340
TIWLLFGFVA ANLTYAAGET HRWYLRKFEN YPANRHAIFP YVY
Length:343
Mass (Da):38,678
Last modified:June 1, 2002 - v2
Checksum:i49AB19FF40A9FA06
GO
Isoform 2 (identifier: Q9SI62-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:342
Mass (Da):38,550
Checksum:i3510ED8C0A5AA451
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66F → L in BX819600 (PubMed:14993207).Curated1
Sequence conflicti69F → L in AAK96489 (PubMed:14593172).Curated1
Sequence conflicti69F → L in AAO11632 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03979358Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007195 Genomic DNA Translation: AAD26491.2
CP002685 Genomic DNA Translation: AEC06507.1
CP002685 Genomic DNA Translation: AEC06508.1
CP002685 Genomic DNA Translation: ANM63071.1
AY052296 mRNA Translation: AAK96489.1
BT002716 mRNA Translation: AAO11632.1
BX819600 mRNA No translation available.
PIRiD84541
RefSeqiNP_001325183.1, NM_001335479.1 [Q9SI62-1]
NP_565389.1, NM_127207.4 [Q9SI62-1]
NP_973474.1, NM_201745.2 [Q9SI62-2]
UniGeneiAt.14490
At.48730

Genome annotation databases

EnsemblPlantsiAT2G16530.1; AT2G16530.1; AT2G16530 [Q9SI62-1]
AT2G16530.2; AT2G16530.2; AT2G16530 [Q9SI62-2]
AT2G16530.4; AT2G16530.4; AT2G16530 [Q9SI62-1]
GeneIDi816152
GrameneiAT2G16530.1; AT2G16530.1; AT2G16530 [Q9SI62-1]
AT2G16530.2; AT2G16530.2; AT2G16530 [Q9SI62-2]
AT2G16530.4; AT2G16530.4; AT2G16530 [Q9SI62-1]
KEGGiath:AT2G16530

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPOED2_ARATH
AccessioniPrimary (citable) accession number: Q9SI62
Secondary accession number(s): Q3EBZ6, Q941B4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: June 1, 2002
Last modified: July 18, 2018
This is version 101 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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