UniProtKB - Q9SI61 (ASE1_ARATH)
Protein
Amidophosphoribosyltransferase 1, chloroplastic
Gene
ASE1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the first committed step of 'de novo' purine biosynthesis from glutamine. Involved in plastid biogenesis and cell division.1 Publication
Catalytic activityi
- 5-phospho-β-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-α-D-ribose 1-diphosphate + H2O + L-glutamineEC:2.4.2.14
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity
- Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Amidophosphoribosyltransferase (AN1_LOCUS20750), Amidophosphoribosyltransferase (AXX17_At4g39730), Amidophosphoribosyltransferase (AN1_LOCUS8003), Amidophosphoribosyltransferase (AT9943_LOCUS17886), Amidophosphoribosyltransferase 2, chloroplastic (ASE2), Amidophosphoribosyltransferase 1, chloroplastic (ASE1), Amidophosphoribosyltransferase 3, chloroplastic (ASE3), Amidophosphoribosyltransferase (AXX17_At4g44210), Amidophosphoribosyltransferase (AXX17_At2g11630), Amidophosphoribosyltransferase (C24_LOCUS20208)
- Glycinamide ribonucleotide synthetase (AXX17_At1g09830), Glycinamide ribonucleotide synthetase (AT9943_LOCUS812), Phosphoribosylamine--glycine ligase, chloroplastic (PUR2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | NucleophilePROSITE-ProRule annotation | 1 | |
Metal bindingi | 327 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 473 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 524 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 527 | Iron-sulfur (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- amidophosphoribosyltransferase activity Source: TAIR
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
- glutamine metabolic process Source: UniProtKB-KW
- nucleoside metabolic process Source: InterPro
- purine nucleobase biosynthetic process Source: TAIR
- purine nucleotide biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine biosynthesis |
Ligand | Iron, Iron-sulfur, Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G16570-MONOMER |
BRENDAi | 2.4.2.14, 399 |
UniPathwayi | UPA00074;UER00124 |
Protein family/group databases
MEROPSi | C44.A01 |
Names & Taxonomyi
Protein namesi | Recommended name: Amidophosphoribosyltransferase 1, chloroplastic (EC:2.4.2.14)Short name: AtATase1 Short name: PRPP1 Alternative name(s): Glutamine phosphoribosylpyrophosphate amidotransferase 1 Short name: AtGPRAT1 |
Gene namesi | Name:ASE1 Synonyms:GPRAT1 Ordered Locus Names:At2g16570 ORF Names:F1P15 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G16570 |
TAIRi | locus:2045081, AT2G16570 |
Subcellular locationi
Chloroplast
- chloroplast stroma 1 Publication
Cell Wall
- cell wall Source: TAIR
Chloroplast
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: TAIR
Other locations
- cytoplasm Source: GO_Central
- plastid Source: TAIR
- plastid stroma Source: UniProtKB
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 58 | ChloroplastSequence analysisAdd BLAST | 58 | |
ChainiPRO_0000420281 | 59 – 566 | Amidophosphoribosyltransferase 1, chloroplasticAdd BLAST | 508 |
Proteomic databases
PaxDbi | Q9SI61 |
PRIDEi | Q9SI61 |
ProteomicsDBi | 246498 |
PTM databases
SwissPalmi | Q9SI61 |
Expressioni
Tissue specificityi
Expressed in flowers and roots. Also present in leaves, and, to a lower extent, in cotyledons.2 Publications
Gene expression databases
ExpressionAtlasi | Q9SI61, baseline and differential |
Genevisiblei | Q9SI61, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 1514, 2 interactors |
IntActi | Q9SI61, 2 interactors |
STRINGi | 3702.AT2G16570.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 91 – 311 | Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST | 221 |
Sequence similaritiesi
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Keywords - Domaini
Glutamine amidotransferase, Transit peptidePhylogenomic databases
eggNOGi | KOG0572, Eukaryota |
HOGENOMi | CLU_022389_3_3_1 |
InParanoidi | Q9SI61 |
OMAi | KGPQDAC |
OrthoDBi | 400911at2759 |
PhylomeDBi | Q9SI61 |
Family and domain databases
CDDi | cd00715, GPATase_N, 1 hit cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit 3.60.20.10, 1 hit |
HAMAPi | MF_01931, PurF, 1 hit |
InterProi | View protein in InterPro IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N IPR000836, PRibTrfase_dom IPR029057, PRTase-like IPR005854, PurF IPR035584, PurF_N |
Pfami | View protein in Pfam PF13537, GATase_7, 1 hit PF00156, Pribosyltran, 1 hit |
SUPFAMi | SSF53271, SSF53271, 1 hit SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01134, purF, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit PS00103, PUR_PYR_PR_TRANSFER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9SI61-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAATTSFSSS LSLITKPNNS SYTNQPLPLF PKPFLKPPHL SLLPSPLSSP
60 70 80 90 100
PPSLIHGVSS YFSSPSPSED NSHTPFDYHN DEDDEKPREE CGVVGIYGDP
110 120 130 140 150
EASRLCYLAL HALQHRGQEG AGIVTVSPEK VLQTITGVGL VSEVFNESKL
160 170 180 190 200
DQLPGEFAIG HVRYSTAGAS MLKNVQPFVA GYRFGSIGVA HNGNLVNYKT
210 220 230 240 250
LRAMLEENGS IFNTSSDTEV VLHLIAISKA RPFFMRIIDA CEKLQGAYSM
260 270 280 290 300
VFVTEDKLVA VRDPYGFRPL VMGRRSNGAV VFASETCALD LIEATYEREV
310 320 330 340 350
YPGEVLVVDK DGVKSQCLMP KFEPKQCIFE HIYFSLPNSI VFGRSVYESR
360 370 380 390 400
HVFGEILATE SPVECDVVIA VPDSGVVAAL GYAAKSGVPF QQGLIRSHYV
410 420 430 440 450
GRTFIEPSQK IRDFGVKLKL SPVRGVLEGK RVVVVDDSIV RGTTSSKIVR
460 470 480 490 500
LLREAGAKEV HMRIASPPIV ASCYYGVDTP SSEELISNRL SVEEINEFIG
510 520 530 540 550
SDSLAFLSFD TLKKHLGKDS KSFCYACFTG DYPVKPTEVK VKRGGGDFID
560
DGLVGSFENI EAGWVR
Sequence cautioni
The sequence BAA06023 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | T → A in BAA06023 (PubMed:7948903).Curated | 1 | |
Sequence conflicti | 106 | C → F in BAA06023 (PubMed:7948903).Curated | 1 | |
Sequence conflicti | 160 | G → A in BAA06023 (PubMed:7948903).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28868 mRNA Translation: BAA06023.1 Frameshift. AC007195 Genomic DNA Translation: AAD26498.1 CP002685 Genomic DNA Translation: AEC06510.1 |
PIRi | E84541 S52622 |
RefSeqi | NP_179247.1, NM_127208.2 |
Genome annotation databases
EnsemblPlantsi | AT2G16570.1; AT2G16570.1; AT2G16570 |
GeneIDi | 816156 |
Gramenei | AT2G16570.1; AT2G16570.1; AT2G16570 |
KEGGi | ath:AT2G16570 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28868 mRNA Translation: BAA06023.1 Frameshift. AC007195 Genomic DNA Translation: AAD26498.1 CP002685 Genomic DNA Translation: AEC06510.1 |
PIRi | E84541 S52622 |
RefSeqi | NP_179247.1, NM_127208.2 |
3D structure databases
SMRi | Q9SI61 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1514, 2 interactors |
IntActi | Q9SI61, 2 interactors |
STRINGi | 3702.AT2G16570.1 |
Protein family/group databases
MEROPSi | C44.A01 |
PTM databases
SwissPalmi | Q9SI61 |
Proteomic databases
PaxDbi | Q9SI61 |
PRIDEi | Q9SI61 |
ProteomicsDBi | 246498 |
Genome annotation databases
EnsemblPlantsi | AT2G16570.1; AT2G16570.1; AT2G16570 |
GeneIDi | 816156 |
Gramenei | AT2G16570.1; AT2G16570.1; AT2G16570 |
KEGGi | ath:AT2G16570 |
Organism-specific databases
Araporti | AT2G16570 |
TAIRi | locus:2045081, AT2G16570 |
Phylogenomic databases
eggNOGi | KOG0572, Eukaryota |
HOGENOMi | CLU_022389_3_3_1 |
InParanoidi | Q9SI61 |
OMAi | KGPQDAC |
OrthoDBi | 400911at2759 |
PhylomeDBi | Q9SI61 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00124 |
BioCyci | ARA:AT2G16570-MONOMER |
BRENDAi | 2.4.2.14, 399 |
Miscellaneous databases
PROi | PR:Q9SI61 |
Gene expression databases
ExpressionAtlasi | Q9SI61, baseline and differential |
Genevisiblei | Q9SI61, AT |
Family and domain databases
CDDi | cd00715, GPATase_N, 1 hit cd06223, PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit 3.60.20.10, 1 hit |
HAMAPi | MF_01931, PurF, 1 hit |
InterProi | View protein in InterPro IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N IPR000836, PRibTrfase_dom IPR029057, PRTase-like IPR005854, PurF IPR035584, PurF_N |
Pfami | View protein in Pfam PF13537, GATase_7, 1 hit PF00156, Pribosyltran, 1 hit |
SUPFAMi | SSF53271, SSF53271, 1 hit SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01134, purF, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit PS00103, PUR_PYR_PR_TRANSFER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASE1_ARATH | |
Accessioni | Q9SI61Primary (citable) accession number: Q9SI61 Secondary accession number(s): Q38999 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2012 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families