UniProtKB - Q9SI17 (PER14_ARATH)
Protein
Peroxidase 14
Gene
PER14
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Miscellaneous
There are 73 peroxidase genes in A.thaliana.
Catalytic activityi
- EC:1.11.1.7
Cofactori
Protein has several cofactor binding sites:- heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
- Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 71 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Active sitei | 75 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 76 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 79 | Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 81 | Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 83 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 85 | Calcium 1PROSITE-ProRule annotation | 1 | |
Binding sitei | 172 | Substrate; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 202 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 203 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 254 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 257 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 262 | Calcium 2PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: UniProtKB-KW
GO - Biological processi
- hydrogen peroxide catabolic process Source: UniProtKB-KW
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide |
Ligand | Calcium, Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G18140-MONOMER |
Protein family/group databases
PeroxiBasei | 95, AtPrx14 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PER14 Synonyms:P14 Ordered Locus Names:At2g18140 ORF Names:F8D23.8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G18140 |
TAIRi | locus:2053139, AT2G18140 |
Subcellular locationi
Extracellular region or secreted
- Secreted PROSITE-ProRule annotation
Cell Wall
- cell wall Source: TAIR
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000023680 | 23 – 337 | Peroxidase 14Add BLAST | 315 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 24 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 44 ↔ 124 | PROSITE-ProRule annotation | ||
Disulfide bondi | 77 ↔ 82 | PROSITE-ProRule annotation | ||
Disulfide bondi | 130 ↔ 331 | PROSITE-ProRule annotation | ||
Glycosylationi | 191 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 209 ↔ 241 | PROSITE-ProRule annotation | ||
Glycosylationi | 218 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 249 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9SI17 |
PRIDEi | Q9SI17 |
ProteomicsDBi | 236455 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9SI17, baseline and differential |
Genevisiblei | Q9SI17, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 1684, 1 interactor |
STRINGi | 3702.AT2G18140.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QR5A, Eukaryota |
HOGENOMi | CLU_010543_0_1_1 |
InParanoidi | Q9SI17 |
OMAi | YAENEEY |
OrthoDBi | 902776at2759 |
PhylomeDBi | Q9SI17 |
Family and domain databases
CDDi | cd00693, secretory_peroxidase, 1 hit |
InterProi | View protein in InterPro IPR002016, Haem_peroxidase IPR010255, Haem_peroxidase_sf IPR000823, Peroxidase_pln IPR019794, Peroxidases_AS IPR019793, Peroxidases_heam-ligand_BS IPR033905, Secretory_peroxidase |
Pfami | View protein in Pfam PF00141, peroxidase, 1 hit |
PRINTSi | PR00458, PEROXIDASE PR00461, PLPEROXIDASE |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS00435, PEROXIDASE_1, 1 hit PS00436, PEROXIDASE_2, 1 hit PS50873, PEROXIDASE_4, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9SI17-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARIGSFLIL LSLTYALTLC ICDNASNFGG NKRNLFPDFY RSSCPRAEEI
60 70 80 90 100
VRSVVAKAFE RETRMAASLM RLHFHDCFVQ GCDGSLLLDT SGSIVTEKNS
110 120 130 140 150
NPNSRSARGF EVVDEIKAAL ENECPNTVSC ADALTLAARD SSVLTGGPSW
160 170 180 190 200
TVPLGRRDSA TASRAKPNKD LPEPDNLFDT IFLRFSNEGL NLTDLVALSG
210 220 230 240 250
SHTIGFSRCT SFRQRLYNQS GSGSPDTTLE KSYAAILRQR CPRSGGDQNL
260 270 280 290 300
SELDINSAGR FDNSYFKNLI ENMGLLNSDQ VLFSSNEQSR ELVKKYAEDQ
310 320 330
EEFFEQFAES MIKMGKISPL TGSSGEIRKK CRKINNS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007212 Genomic DNA Translation: AAD31352.1 CP002685 Genomic DNA Translation: AEC06730.1 |
PIRi | G84560 |
RefSeqi | NP_179406.1, NM_127371.1 |
Genome annotation databases
EnsemblPlantsi | AT2G18140.1; AT2G18140.1; AT2G18140 |
GeneIDi | 816327 |
Gramenei | AT2G18140.1; AT2G18140.1; AT2G18140 |
KEGGi | ath:AT2G18140 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007212 Genomic DNA Translation: AAD31352.1 CP002685 Genomic DNA Translation: AEC06730.1 |
PIRi | G84560 |
RefSeqi | NP_179406.1, NM_127371.1 |
3D structure databases
SMRi | Q9SI17 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1684, 1 interactor |
STRINGi | 3702.AT2G18140.1 |
Protein family/group databases
PeroxiBasei | 95, AtPrx14 |
Proteomic databases
PaxDbi | Q9SI17 |
PRIDEi | Q9SI17 |
ProteomicsDBi | 236455 |
Genome annotation databases
EnsemblPlantsi | AT2G18140.1; AT2G18140.1; AT2G18140 |
GeneIDi | 816327 |
Gramenei | AT2G18140.1; AT2G18140.1; AT2G18140 |
KEGGi | ath:AT2G18140 |
Organism-specific databases
Araporti | AT2G18140 |
TAIRi | locus:2053139, AT2G18140 |
Phylogenomic databases
eggNOGi | ENOG502QR5A, Eukaryota |
HOGENOMi | CLU_010543_0_1_1 |
InParanoidi | Q9SI17 |
OMAi | YAENEEY |
OrthoDBi | 902776at2759 |
PhylomeDBi | Q9SI17 |
Enzyme and pathway databases
BioCyci | ARA:AT2G18140-MONOMER |
Miscellaneous databases
PROi | PR:Q9SI17 |
Gene expression databases
ExpressionAtlasi | Q9SI17, baseline and differential |
Genevisiblei | Q9SI17, AT |
Family and domain databases
CDDi | cd00693, secretory_peroxidase, 1 hit |
InterProi | View protein in InterPro IPR002016, Haem_peroxidase IPR010255, Haem_peroxidase_sf IPR000823, Peroxidase_pln IPR019794, Peroxidases_AS IPR019793, Peroxidases_heam-ligand_BS IPR033905, Secretory_peroxidase |
Pfami | View protein in Pfam PF00141, peroxidase, 1 hit |
PRINTSi | PR00458, PEROXIDASE PR00461, PLPEROXIDASE |
SUPFAMi | SSF48113, SSF48113, 1 hit |
PROSITEi | View protein in PROSITE PS00435, PEROXIDASE_1, 1 hit PS00436, PEROXIDASE_2, 1 hit PS50873, PEROXIDASE_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PER14_ARATH | |
Accessioni | Q9SI17Primary (citable) accession number: Q9SI17 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 25, 2002 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families