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Protein

Probable copper-transporting ATPase HMA5

Gene

HMA5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in copper import into the cell. May play a role in copper detoxification in roots.1 Publication

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62CopperPROSITE-ProRule annotation1
Metal bindingi65CopperPROSITE-ProRule annotation1
Metal bindingi140CopperPROSITE-ProRule annotation1
Metal bindingi143CopperPROSITE-ProRule annotation1
Active sitei6614-aspartylphosphate intermediateBy similarity1
Metal bindingi866MagnesiumPROSITE-ProRule annotation1
Metal bindingi870MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • copper ion transport Source: UniProtKB-KW
  • detoxification of copper ion Source: TAIR
  • response to copper ion Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processCopper transport, Ion transport, Transport
LigandATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G63440-MONOMER
ARA:MONOMER-14503
MetaCyc:MONOMER-14503
BRENDAi3.6.3.4 399
ReactomeiR-ATH-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Probable copper-transporting ATPase HMA5 (EC:3.6.3.54)
Alternative name(s):
Probable copper-transporting ATPase 3
Protein HEAVY METAL ATPASE 5
Gene namesi
Name:HMA5
Ordered Locus Names:At1g63440
ORF Names:F2K11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G63440
TAIRilocus:2031361 AT1G63440

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 299CytoplasmicSequence analysisAdd BLAST299
Transmembranei300 – 321HelicalSequence analysisAdd BLAST22
Topological domaini322 – 340ExtracellularSequence analysisAdd BLAST19
Transmembranei341 – 360HelicalSequence analysisAdd BLAST20
Topological domaini361 – 367CytoplasmicSequence analysis7
Transmembranei368 – 388HelicalSequence analysisAdd BLAST21
Topological domaini389 – 406ExtracellularSequence analysisAdd BLAST18
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21
Topological domaini428 – 561CytoplasmicSequence analysisAdd BLAST134
Transmembranei562 – 584HelicalSequence analysisAdd BLAST23
Topological domaini585 – 605ExtracellularSequence analysisAdd BLAST21
Transmembranei606 – 623HelicalSequence analysisAdd BLAST18
Topological domaini624 – 920CytoplasmicSequence analysisAdd BLAST297
Transmembranei921 – 940HelicalSequence analysisAdd BLAST20
Topological domaini941 – 952ExtracellularSequence analysisAdd BLAST12
Transmembranei953 – 971HelicalSequence analysisAdd BLAST19
Topological domaini972 – 995CytoplasmicSequence analysisAdd BLAST24

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants are hypersensitive to copper excess.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464041 – 995Probable copper-transporting ATPase HMA5Add BLAST995

Proteomic databases

PaxDbiQ9SH30
PRIDEiQ9SH30

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Inductioni

By copper.1 Publication

Gene expression databases

ExpressionAtlasiQ9SH30 baseline and differential
GenevisibleiQ9SH30 AT

Interactioni

Subunit structurei

Interacts with ATX1.1 Publication

Protein-protein interaction databases

BioGridi27871, 2 interactors
IntActiQ9SH30, 1 interactor
STRINGi3702.AT1G63440.1

Structurei

3D structure databases

ProteinModelPortaliQ9SH30
SMRiQ9SH30
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 118HMA 1PROSITE-ProRule annotationAdd BLAST67
Domaini130 – 196HMA 2PROSITE-ProRule annotationAdd BLAST67
Domaini205 – 271HMA 3; degeneratePROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207 Eukaryota
COG2217 LUCA
HOGENOMiHOG000250397
InParanoidiQ9SH30
KOiK17686
OMAiHIFGGPL
OrthoDBiEOG093602FP
PhylomeDBiQ9SH30

Family and domain databases

CDDicd00371 HMA, 3 hits
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR017969 Heavy-metal-associated_CS
IPR006122 HMA_Cu_ion-bd
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 3 hits
SUPFAMiSSF55008 SSF55008, 3 hits
SSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 1 hit
TIGR00003 TIGR00003, 1 hit
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS01047 HMA_1, 1 hit
PS50846 HMA_2, 3 hits

Sequencei

Sequence statusi: Complete.

Q9SH30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKLLSLTC IRKERFSERY PLVRKHLTRS RDGGGGSSSE TAAFEIDDPI
60 70 80 90 100
SRAVFQVLGM TCSACAGSVE KAIKRLPGIH DAVIDALNNR AQILFYPNSV
110 120 130 140 150
DVETIRETIE DAGFEASLIE NEANERSRQV CRIRINGMTC TSCSSTIERV
160 170 180 190 200
LQSVNGVQRA HVALAIEEAE IHYDPRLSSY DRLLEEIENA GFEAVLISTG
210 220 230 240 250
EDVSKIDLKI DGELTDESMK VIERSLEALP GVQSVEISHG TDKISVLYKP
260 270 280 290 300
DVTGPRNFIQ VIESTVFGHS GHIKATIFSE GGVGRESQKQ GEIKQYYKSF
310 320 330 340 350
LWSLVFTVPV FLTAMVFMYI PGIKDLLMFK VINMLTVGEI IRCVLATPVQ
360 370 380 390 400
FVIGWRFYTG SYKALRRGSA NMDVLIALGT NAAYFYSLYT VLRAATSPDF
410 420 430 440 450
KGVDFFETSA MLISFIILGK YLEVMAKGKT SQAIAKLMNL APDTAILLSL
460 470 480 490 500
DKEGNVTGEE EIDGRLIQKN DVIKIVPGAK VASDGYVIWG QSHVNESMIT
510 520 530 540 550
GEARPVAKRK GDTVIGGTLN ENGVLHVKVT RVGSESALAQ IVRLVESAQL
560 570 580 590 600
AKAPVQKLAD RISKFFVPLV IFLSFSTWLA WFLAGKLHWY PESWIPSSMD
610 620 630 640 650
SFELALQFGI SVMVIACPCA LGLATPTAVM VGTGVGASQG VLIKGGQALE
660 670 680 690 700
RAHKVNCIVF DKTGTLTMGK PVVVKTKLLK NMVLREFYEL VAATEVNSEH
710 720 730 740 750
PLAKAIVEYA KKFRDDEENP AWPEACDFVS ITGKGVKATV KGREIMVGNK
760 770 780 790 800
NLMNDHKVII PDDAEELLAD SEDMAQTGIL VSINSELIGV LSVSDPLKPS
810 820 830 840 850
AREAISILKS MNIKSIMVTG DNWGTANSIA REVGIDSVIA EAKPEQKAEK
860 870 880 890 900
VKELQAAGHV VAMVGDGIND SPALVAADVG MAIGAGTDIA IEAADIVLMK
910 920 930 940 950
SNLEDVITAI DLSRKTFSRI RLNYVWALGY NLMGIPIAAG VLFPGTRFRL
960 970 980 990
PPWIAGAAMA ASSVSVVCCS LLLKNYKRPK KLDHLEIREI QVERV
Length:995
Mass (Da):108,336
Last modified:January 11, 2001 - v2
Checksum:iC9289E6FDE7D727B
GO

Sequence cautioni

The sequence AAF19707 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008047 Genomic DNA Translation: AAF19707.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34100.1
PIRiB96660
RefSeqiNP_176533.1, NM_105023.2

Genome annotation databases

EnsemblPlantsiAT1G63440.1; AT1G63440.1; AT1G63440
GeneIDi842650
GrameneiAT1G63440.1; AT1G63440.1; AT1G63440
KEGGiath:AT1G63440

Similar proteinsi

Entry informationi

Entry nameiHMA5_ARATH
AccessioniPrimary (citable) accession number: Q9SH30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: May 23, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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