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UniProtKB - Q9SFH9 (HEM21_ARATH)
Protein
Delta-aminolevulinic acid dehydratase 1, chloroplastic
Gene
HEMB1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).
By similarityCatalytic activityi
- EC:4.2.1.24
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator.By similarity
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate. This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
Pathwayi: chlorophyll biosynthesis
This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 298 | Schiff-base intermediate with substrateBy similarity | 1 | |
Binding sitei | 308 | 5-aminolevulinate 1By similarity | 1 | |
Binding sitei | 320 | 5-aminolevulinate 1By similarity | 1 | |
Metal bindingi | 336 | MagnesiumBy similarity | 1 | |
Active sitei | 351 | Schiff-base intermediate with substrateBy similarity | 1 | |
Binding sitei | 377 | 5-aminolevulinate 2By similarity | 1 | |
Binding sitei | 416 | 5-aminolevulinate 2By similarity | 1 |
GO - Molecular functioni
- porphobilinogen synthase activity Source: GO_Central
- zinc ion binding Source: GO_Central
GO - Biological processi
- chlorophyll biosynthetic process Source: UniProtKB-UniPathway
- heme biosynthetic process Source: GO_Central
- porphyrin-containing compound biosynthetic process Source: TAIR
- protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Allosteric enzyme, Lyase |
Biological process | Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00318 UPA00668 |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-aminolevulinic acid dehydratase 1, chloroplastic (EC:4.2.1.24)Short name: ALADH1 Alternative name(s): Porphobilinogen synthase |
Gene namesi | Name:HEMB1 Ordered Locus Names:At1g69740 ORF Names:T6C23.6 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G69740 |
TAIRi | locus:2205035, AT1G69740 |
Subcellular locationi
Chloroplast
- chloroplast By similarity
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrion Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Embryo lethal when homozygous. Impaired plant growth and development.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 52 | ChloroplastSequence analysisAdd BLAST | 52 | |
ChainiPRO_0000013314 | 53 – 430 | Delta-aminolevulinic acid dehydratase 1, chloroplasticAdd BLAST | 378 |
Proteomic databases
PaxDbi | Q9SFH9 |
PRIDEi | Q9SFH9 |
ProteomicsDBi | 228741 |
Expressioni
Tissue specificityi
Highly expressed in cotyledons during dark-to-light transition.1 Publication
Inductioni
Up-regulated by the transcription factors FAR1 and FHY3.1 Publication
Gene expression databases
ExpressionAtlasi | Q9SFH9, baseline and differential |
Genevisiblei | Q9SFH9, AT |
Interactioni
Subunit structurei
Homooctamer.
By similarityProtein-protein interaction databases
BioGRIDi | 28531, 2 interactors |
STRINGi | 3702.AT1G69740.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 82 – 101 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the ALAD family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2794, Eukaryota |
HOGENOMi | CLU_035731_1_0_1 |
InParanoidi | Q9SFH9 |
OMAi | YQMDYAN |
OrthoDBi | 918089at2759 |
PhylomeDBi | Q9SFH9 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR001731, ALAD IPR030656, ALAD_AS IPR013785, Aldolase_TIM |
PANTHERi | PTHR11458, PTHR11458, 1 hit |
Pfami | View protein in Pfam PF00490, ALAD, 1 hit |
PRINTSi | PR00144, DALDHYDRTASE |
SMARTi | View protein in SMART SM01004, ALAD, 1 hit |
PROSITEi | View protein in PROSITE PS00169, D_ALA_DEHYDRATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9SFH9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATTPIFNAS CSFPSTRGID CKSYIGLRSN VSKVSVASSR IATSQRRNLV
60 70 80 90 100
VRASESGNGH AKKLGMSDAE CEAAVAAGNV PEAPPVPPKP AAPVGTPIIK
110 120 130 140 150
PLNLSRRPRR NRASPVTRAA FQETDISPAN FVYPLFIHEG EEDTPIGAMP
160 170 180 190 200
GCYRLGWRHG LVQEVAKARA VGVNSIVLFP KVPEALKNST GDEAYNDNGL
210 220 230 240 250
VPRTIRLLKD KYPDLIIYTD VALDPYSSDG HDGIVREDGV IMNDETVHQL
260 270 280 290 300
CKQAVSQARA GADVVSPSDM MDGRVGAIRS ALDAEGFQNV SIMSYTAKYA
310 320 330 340 350
SSFYGPFREA LDSNPRFGDK KTYQMNPANY REALIEARED EAEGADILLV
360 370 380 390 400
KPGLPYLDII RLLRDKSPLP IAAYQVSGEY SMIKAGGVLK MIDEEKVMME
410 420 430
SLMCLRRAGA DIILTYFALQ AATCLCGEKR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC013289 Genomic DNA Translation: AAG52549.1 CP002684 Genomic DNA Translation: AEE34969.1 CP002684 Genomic DNA Translation: AEE34970.1 CP002684 Genomic DNA Translation: ANM58523.1 AF327428 mRNA Translation: AAG42018.1 AF361803 mRNA Translation: AAK32816.1 AY059154 mRNA Translation: AAL15379.1 AY081254 mRNA Translation: AAL91143.1 AY113941 mRNA Translation: AAM44989.1 AY120705 mRNA Translation: AAM53263.1 AY128711 mRNA Translation: AAM91111.1 |
PIRi | D96719 |
RefSeqi | NP_001077800.1, NM_001084331.3 NP_001320950.1, NM_001334421.1 NP_177132.1, NM_105642.4 |
Genome annotation databases
EnsemblPlantsi | AT1G69740.1; AT1G69740.1; AT1G69740 AT1G69740.2; AT1G69740.2; AT1G69740 AT1G69740.3; AT1G69740.3; AT1G69740 |
GeneIDi | 843310 |
Gramenei | AT1G69740.1; AT1G69740.1; AT1G69740 AT1G69740.2; AT1G69740.2; AT1G69740 AT1G69740.3; AT1G69740.3; AT1G69740 |
KEGGi | ath:AT1G69740 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC013289 Genomic DNA Translation: AAG52549.1 CP002684 Genomic DNA Translation: AEE34969.1 CP002684 Genomic DNA Translation: AEE34970.1 CP002684 Genomic DNA Translation: ANM58523.1 AF327428 mRNA Translation: AAG42018.1 AF361803 mRNA Translation: AAK32816.1 AY059154 mRNA Translation: AAL15379.1 AY081254 mRNA Translation: AAL91143.1 AY113941 mRNA Translation: AAM44989.1 AY120705 mRNA Translation: AAM53263.1 AY128711 mRNA Translation: AAM91111.1 |
PIRi | D96719 |
RefSeqi | NP_001077800.1, NM_001084331.3 NP_001320950.1, NM_001334421.1 NP_177132.1, NM_105642.4 |
3D structure databases
AlphaFoldDBi | Q9SFH9 |
SMRi | Q9SFH9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 28531, 2 interactors |
STRINGi | 3702.AT1G69740.1 |
Proteomic databases
PaxDbi | Q9SFH9 |
PRIDEi | Q9SFH9 |
ProteomicsDBi | 228741 |
Genome annotation databases
EnsemblPlantsi | AT1G69740.1; AT1G69740.1; AT1G69740 AT1G69740.2; AT1G69740.2; AT1G69740 AT1G69740.3; AT1G69740.3; AT1G69740 |
GeneIDi | 843310 |
Gramenei | AT1G69740.1; AT1G69740.1; AT1G69740 AT1G69740.2; AT1G69740.2; AT1G69740 AT1G69740.3; AT1G69740.3; AT1G69740 |
KEGGi | ath:AT1G69740 |
Organism-specific databases
Araporti | AT1G69740 |
TAIRi | locus:2205035, AT1G69740 |
Phylogenomic databases
eggNOGi | KOG2794, Eukaryota |
HOGENOMi | CLU_035731_1_0_1 |
InParanoidi | Q9SFH9 |
OMAi | YQMDYAN |
OrthoDBi | 918089at2759 |
PhylomeDBi | Q9SFH9 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00318 UPA00668 |
Miscellaneous databases
PROi | PR:Q9SFH9 |
Gene expression databases
ExpressionAtlasi | Q9SFH9, baseline and differential |
Genevisiblei | Q9SFH9, AT |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR001731, ALAD IPR030656, ALAD_AS IPR013785, Aldolase_TIM |
PANTHERi | PTHR11458, PTHR11458, 1 hit |
Pfami | View protein in Pfam PF00490, ALAD, 1 hit |
PRINTSi | PR00144, DALDHYDRTASE |
SMARTi | View protein in SMART SM01004, ALAD, 1 hit |
PROSITEi | View protein in PROSITE PS00169, D_ALA_DEHYDRATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HEM21_ARATH | |
Accessioni | Q9SFH9Primary (citable) accession number: Q9SFH9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families