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UniProtKB - Q9SEV0 (BAN_ARATH)
Protein
Anthocyanidin reductase
Gene
BAN
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in the biosynthesis of condensed tannins. Converts cyanidin into (-)-epicatechin as the major product.
2 PublicationsMiscellaneous
Regulated by TRANSPARENT TESTA 2, TRANSPARENT TESTA 8 and TRANSPARENT TESTA GLABRA 1.
Catalytic activityi
- a (2R,3R)-flavan-3-ol + 2 NAD+ = an anthocyanidin with a 3-hydroxy group + 2 H+ + 2 NADH2 PublicationsEC:1.3.1.772 Publications
- a (2R,3R)-flavan-3-ol + 2 NADP+ = an anthocyanidin with a 3-hydroxy group + 2 H+ + 2 NADPH2 PublicationsEC:1.3.1.772 Publications
Activity regulationi
Inhibited by (+)-catechin, quercetin and (+)- and (-)-dihydroquercetin. Not inhibited by salt. Positive cooperativity with NADPH acting as cosubstrate and modulator.1 Publication
Kineticsi
cannot use NADH as cosubstrate.
- KM=73.9 µM for cyanidin1 Publication
- KM=52.8 µM for pelargonidin1 Publication
- KM=17.8 µM for delphinidin1 Publication
- Vmax=10.0 nmol/min/mg enzyme with cyanidin as substrate1 Publication
- Vmax=0.9 nmol/min/mg enzyme with delphinidin as substrate1 Publication
pH dependencei
Optimum pH is 8.0.1 Publication
Temperature dependencei
Optimum temperature is 55 degrees Celsius.1 Publication
: flavonoid biosynthesis Pathwayi
This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 49 | NADPBy similarity | 1 | |
Binding sitei | 169 | NADPBy similarity | 1 |
GO - Molecular functioni
- anthocyanidin reductase activity Source: TAIR
- oxidoreductase activity Source: TAIR
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: GO_Central
GO - Biological processi
- flavonoid biosynthetic process Source: UniProtKB-UniPathway
- negative regulation of flavonoid biosynthetic process Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Flavonoid biosynthesis |
Ligand | NAD, NADP |
Enzyme and pathway databases
BioCyci | ARA:AT1G61720-MONOMER |
BRENDAi | 1.3.1.77, 399 |
SABIO-RKi | Q9SEV0 |
UniPathwayi | UPA00154 |
Names & Taxonomyi
Protein namesi | Recommended name: Anthocyanidin reductase (EC:1.3.1.772 Publications)Short name: AtANR1 Publication Alternative name(s): Anthocyanin spotted testa Short name: ast Protein BANYULS |
Gene namesi | Name:BAN Synonyms:ANR Ordered Locus Names:At1g61720 ORF Names:T13M11.8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G61720 |
TAIRi | locus:2195733, AT1G61720 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215574 | 1 – 340 | Anthocyanidin reductaseAdd BLAST | 340 |
Proteomic databases
PaxDbi | Q9SEV0 |
ProteomicsDBi | 241210 |
Expressioni
Tissue specificityi
Flowers and young siliques. Detected specifically in the endothelium of seed coat.1 Publication
Developmental stagei
From fertilization until pre-globular embryo stage.
Gene expression databases
ExpressionAtlasi | Q9SEV0, baseline and differential |
Genevisiblei | Q9SEV0, AT |
Interactioni
Subunit structurei
Homo- or heterodimer.
CuratedProtein-protein interaction databases
STRINGi | 3702.AT1G61720.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1502, Eukaryota |
HOGENOMi | CLU_007383_9_0_1 |
InParanoidi | Q9SEV0 |
OMAi | VFQLATP |
OrthoDBi | 992332at2759 |
PhylomeDBi | Q9SEV0 |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SEV0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDQTLTHTGS KKACVIGGTG NLASILIKHL LQSGYKVNTT VRDPENEKKI
60 70 80 90 100
AHLRKLQELG DLKIFKADLT DEDSFESSFS GCEYIFHVAT PINFKSEDPE
110 120 130 140 150
KDMIKPAIQG VINVLKSCLK SKSVKRVIYT SSAAAVSINN LSGTGIVMNE
160 170 180 190 200
ENWTDVEFLT EEKPFNWGYP ISKVLAEKTA WEFAKENKIN LVTVIPALIA
210 220 230 240 250
GNSLLSDPPS SLSLSMSFIT GKEMHVTGLK EMQKLSGSIS FVHVDDLARA
260 270 280 290 300
HLFLAEKETA SGRYICCAYN TSVPEIADFL IQRYPKYNVL SEFEEGLSIP
310 320 330 340
KLTLSSQKLI NEGFRFEYGI NEMYDQMIEY FESKGLIKAK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 55 | K → Q in AAF23859 (PubMed:10504561).Curated | 1 | |
Sequence conflicti | 146 | I → L in AAF23859 (PubMed:10504561).Curated | 1 | |
Sequence conflicti | 156 – 157 | VE → ID in AAF23859 (PubMed:10504561).Curated | 2 | |
Sequence conflicti | 179 | T → K in AAF23859 (PubMed:10504561).Curated | 1 | |
Sequence conflicti | 185 | K → E in AAF23859 (PubMed:10504561).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 340 | K → KES in strain: cv. Wassilewskija. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092912 mRNA Translation: AAF23859.1 AC005882 Genomic DNA Translation: AAD21417.1 CP002684 Genomic DNA Translation: AEE33879.1 DQ446384 mRNA Translation: ABE65732.1 AK175960 mRNA Translation: BAD43723.1 |
PIRi | H96642 |
RefSeqi | NP_176365.1, NM_104854.4 |
Genome annotation databases
EnsemblPlantsi | AT1G61720.1; AT1G61720.1; AT1G61720 |
GeneIDi | 842469 |
Gramenei | AT1G61720.1; AT1G61720.1; AT1G61720 |
KEGGi | ath:AT1G61720 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF092912 mRNA Translation: AAF23859.1 AC005882 Genomic DNA Translation: AAD21417.1 CP002684 Genomic DNA Translation: AEE33879.1 DQ446384 mRNA Translation: ABE65732.1 AK175960 mRNA Translation: BAD43723.1 |
PIRi | H96642 |
RefSeqi | NP_176365.1, NM_104854.4 |
3D structure databases
SMRi | Q9SEV0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G61720.1 |
Proteomic databases
PaxDbi | Q9SEV0 |
ProteomicsDBi | 241210 |
Genome annotation databases
EnsemblPlantsi | AT1G61720.1; AT1G61720.1; AT1G61720 |
GeneIDi | 842469 |
Gramenei | AT1G61720.1; AT1G61720.1; AT1G61720 |
KEGGi | ath:AT1G61720 |
Organism-specific databases
Araporti | AT1G61720 |
TAIRi | locus:2195733, AT1G61720 |
Phylogenomic databases
eggNOGi | KOG1502, Eukaryota |
HOGENOMi | CLU_007383_9_0_1 |
InParanoidi | Q9SEV0 |
OMAi | VFQLATP |
OrthoDBi | 992332at2759 |
PhylomeDBi | Q9SEV0 |
Enzyme and pathway databases
UniPathwayi | UPA00154 |
BioCyci | ARA:AT1G61720-MONOMER |
BRENDAi | 1.3.1.77, 399 |
SABIO-RKi | Q9SEV0 |
Miscellaneous databases
PROi | PR:Q9SEV0 |
Gene expression databases
ExpressionAtlasi | Q9SEV0, baseline and differential |
Genevisiblei | Q9SEV0, AT |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BAN_ARATH | |
Accessioni | Q9SEV0Primary (citable) accession number: Q9SEV0 Secondary accession number(s): Q1PFH9, Q680A7, Q9SYA8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 13, 2002 |
Last sequence update: | December 13, 2002 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families