UniProtKB - Q9SE83 (DRP2A_ARATH)
Protein
Dynamin-2A
Gene
DRP2A
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Microtubule-associated force-producing protein involved in clathrin-mediated vesicle trafficking from the trans-Golgi network to the central vacuole. Able to bind and hydrolyze GTP. Binds specifically to phosphatidylinositol 3-phosphate (PtdIns3P).3 Publications
Catalytic activityi
- EC:3.6.5.5
Activity regulationi
Increased GTPase activity in the presence of phosphatidic acid.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 52 | GTPSequence analysis | 8 | |
Nucleotide bindingi | 143 – 147 | GTPBy similarity | 5 | |
Nucleotide bindingi | 204 – 207 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: TAIR
- GTP binding Source: UniProtKB-KW
- lipid binding Source: UniProtKB-KW
- microtubule binding Source: GO_Central
GO - Biological processi
- Golgi to vacuole transport Source: TAIR
- mitochondrial fission Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Motor protein |
Ligand | GTP-binding, Lipid-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Dynamin-2A (EC:3.6.5.5)Alternative name(s): Dynamin-like protein 6 Dynamin-related protein 2A |
Gene namesi | Name:DRP2A Synonyms:ADL6 Ordered Locus Names:At1g10290 ORF Names:F14N23.17 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G10290 |
TAIRi | locus:2012763, AT1G10290 |
Subcellular locationi
Cytoplasm and Cytosol
Golgi apparatus
Cytoskeleton
Other locations
- clathrin-coated vesicle 1 Publication
Note: Localized in the forming cell plate during cytokinesis.
Cytoskeleton
- microtubule Source: UniProtKB-KW
Cytosol
- cytosol Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: TAIR
Golgi apparatus
- Golgi apparatus Source: TAIR
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: TAIR
Vacuole
- vacuolar membrane Source: TAIR
Other locations
- clathrin-coated vesicle Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
- membrane Source: GO_Central
- phragmoplast Source: UniProtKB-SubCell
- plasmodesma Source: TAIR
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Golgi apparatus, Membrane, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 51 | K → E: Affects the trafficking between the Golgi apparatus and the vacuole. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206582 | 1 – 914 | Dynamin-2AAdd BLAST | 914 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q9SE83 |
PRIDEi | Q9SE83 |
ProteomicsDBi | 224364 |
PTM databases
iPTMneti | Q9SE83 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9SE83, baseline and differential |
Genevisiblei | Q9SE83, AT |
Interactioni
Subunit structurei
GO - Molecular functioni
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 22808, 12 interactors |
IntActi | Q9SE83, 2 interactors |
STRINGi | 3702.AT1G10290.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 303 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 269 | |
Domaini | 572 – 696 | PHPROSITE-ProRule annotationAdd BLAST | 125 | |
Domaini | 730 – 823 | GEDPROSITE-ProRule annotationAdd BLAST | 94 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 45 – 52 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 71 – 73 | G2 motifPROSITE-ProRule annotation | 3 | |
Regioni | 143 – 146 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 204 – 207 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 238 – 241 | G5 motifPROSITE-ProRule annotation | 4 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 781 – 805 | Sequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 879 – 914 | Pro-richAdd BLAST | 36 |
Domaini
The PH domain binds phospholipids. The PRD1 motif (721-728) is necessary for the interaction with SH3P3.1 Publication
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0446, Eukaryota |
HOGENOMi | CLU_016157_1_0_1 |
InParanoidi | Q9SE83 |
OMAi | KMVRKPI |
OrthoDBi | 162674at2759 |
PhylomeDBi | Q9SE83 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11566, PTHR11566, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit PS50003, PH_DOMAIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SE83-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAIDELSQL SDSMKQAASL LADEDPDETS SSKRPATFLN VVALGNVGAG
60 70 80 90 100
KSAVLNSLIG HPVLPTGENG ATRAPIIIEL SRESSLSSKA IILQIDNKSQ
110 120 130 140 150
QVSASALRHS LQDRLSKGAS GKNRDEINLK LRTSTAPPLK LVDLPGLDQR
160 170 180 190 200
IVDESMIAEY AQHNDAILLV IVPASQASEI SSSRALKIAK EYDPESTRTI
210 220 230 240 250
GIIGKIDQAA ENSKALAAVQ ALLSNQGPPK TTDIPWVAVI GQSVSIASAQ
260 270 280 290 300
SGSGENSLET AWRAESESLK SILTGAPQSK LGRIALVDTL ASQIRSRMKL
310 320 330 340 350
RLPSVLSGLQ GKSQIVQDEL ARLGEQLVNS AEGTRAIALE LCREFEDKFL
360 370 380 390 400
LHLAGGEGSG WKVVASFEGN FPNRIKQLPL DRHFDLNNVK RVVLEADGYQ
410 420 430 440 450
PYLISPEKGL RSLIKIVLEL AKDPARLCVD EVHRVLVDIV SASANATPGL
460 470 480 490 500
GRYPPFKREV VAIASAALDG FKNEAKKMVV ALVDMERAFV PPQHFIRLVQ
510 520 530 540 550
RRMERQRREE ELKGRSSKKG QDAEQSLLSR ATSPQPDGPT AGGSLKSMKD
560 570 580 590 600
KPSPQDKETP EVSGLKTAGP EGEITAGYLM KKSAKTNGWS RRWFVLNEKT
610 620 630 640 650
GKLGYTKKQE ERNFRGTITL EECTIEEIPE DEVEKSKSSK DKKANGPDSK
660 670 680 690 700
GPGLVFKITC KVPYKTVLKA HNALVLKAES VVDKNEWINK LQKVIQARGG
710 720 730 740 750
QVGSVSMRQS LSEGSLDKMV RKPIDPEEEL RWMSQEVRGY VEAVLNSLAA
760 770 780 790 800
NVPKAVVLCQ VEKAKEDMLN QLYSSISAIG NERIESLIQE DQNVKRRRER
810 820 830 840 850
YQKQSSLLSK LTRQLSIHDN RAAAASSYSD NSGTESSPRA SGGSSGDDWM
860 870 880 890 900
NAFNSAANGP SDSLSKYGSG GHSRRYSDPA QNGDAASPGS GSNRRTTPNR
910
LPPAPPPTGS AYRY
Sequence cautioni
The sequence AAD32879 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 617 | T → S in BAD95292 (Ref. 4) Curated | 1 | |
Sequence conflicti | 622 | E → V in BAF00882 (Ref. 4) Curated | 1 | |
Sequence conflicti | 633 | V → G in BAD95292 (Ref. 4) Curated | 1 | |
Sequence conflicti | 840 | A → T in AAF22291 (PubMed:11449048).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF180732 mRNA Translation: AAF22291.1 AC005489 Genomic DNA Translation: AAD32879.1 Sequence problems. CP002684 Genomic DNA Translation: AEE28562.1 AK221193 mRNA Translation: BAD95292.1 AK228995 mRNA Translation: BAF00882.1 |
PIRi | B86237 |
RefSeqi | NP_172500.1, NM_100903.4 |
Genome annotation databases
EnsemblPlantsi | AT1G10290.1; AT1G10290.1; AT1G10290 |
GeneIDi | 837568 |
Gramenei | AT1G10290.1; AT1G10290.1; AT1G10290 |
KEGGi | ath:AT1G10290 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF180732 mRNA Translation: AAF22291.1 AC005489 Genomic DNA Translation: AAD32879.1 Sequence problems. CP002684 Genomic DNA Translation: AEE28562.1 AK221193 mRNA Translation: BAD95292.1 AK228995 mRNA Translation: BAF00882.1 |
PIRi | B86237 |
RefSeqi | NP_172500.1, NM_100903.4 |
3D structure databases
SMRi | Q9SE83 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 22808, 12 interactors |
IntActi | Q9SE83, 2 interactors |
STRINGi | 3702.AT1G10290.1 |
PTM databases
iPTMneti | Q9SE83 |
Proteomic databases
PaxDbi | Q9SE83 |
PRIDEi | Q9SE83 |
ProteomicsDBi | 224364 |
Genome annotation databases
EnsemblPlantsi | AT1G10290.1; AT1G10290.1; AT1G10290 |
GeneIDi | 837568 |
Gramenei | AT1G10290.1; AT1G10290.1; AT1G10290 |
KEGGi | ath:AT1G10290 |
Organism-specific databases
Araporti | AT1G10290 |
TAIRi | locus:2012763, AT1G10290 |
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
HOGENOMi | CLU_016157_1_0_1 |
InParanoidi | Q9SE83 |
OMAi | KMVRKPI |
OrthoDBi | 162674at2759 |
PhylomeDBi | Q9SE83 |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 399 |
Miscellaneous databases
PROi | PR:Q9SE83 |
Gene expression databases
ExpressionAtlasi | Q9SE83, baseline and differential |
Genevisiblei | Q9SE83, AT |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11566, PTHR11566, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DRP2A_ARATH | |
Accessioni | Q9SE83Primary (citable) accession number: Q9SE83 Secondary accession number(s): Q0WPR7, Q56YX7, Q9SY71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | February 1, 2005 | |
Last modified: | April 7, 2021 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families