UniProtKB - Q9SE42 (RPE1_ORYSJ)
Protein
Ribulose-phosphate 3-epimerase, cytoplasmic isoform
Gene
Os09g0505700
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.By similarity
Catalytic activityi
- EC:5.1.3.1By similarity
Cofactori
Co2+By similarity, Fe2+By similarity, Mn2+By similarity, Zn2+1 PublicationNote: Binds 1 divalent metal cation per subunit. Active with Co2+, Fe2+, Mn2+ and Zn2+.1 Publication
Kineticsi
- Vmax=16000 µmol/min/mg enzyme1 Publication
: pentose phosphate pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage).Proteins known to be involved in this subpathway in this organism are:
- Ribulose-phosphate 3-epimerase, cytoplasmic isoform (Os09g0505700)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | SubstrateBy similarity | 1 | |
Metal bindingi | 37 | Divalent metal cation; via tele nitrogenCombined sources1 Publication | 1 | |
Active sitei | 39 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 39 | Divalent metal cationCombined sources1 Publication | 1 | |
Metal bindingi | 70 | Divalent metal cation; via pros nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 70 | SubstrateBy similarity | 1 | |
Active sitei | 179 | Proton donorBy similarity | 1 | |
Metal bindingi | 179 | Divalent metal cationCombined sources1 Publication | 1 |
GO - Molecular functioni
- metal ion binding Source: GO_Central
- ribulose-phosphate 3-epimerase activity Source: GO_Central
GO - Biological processi
- cellular carbohydrate metabolic process Source: GO_Central
- pentose catabolic process Source: GO_Central
- pentose-phosphate shunt, non-oxidative branch Source: GO_Central
Keywordsi
Molecular function | Isomerase |
Biological process | Carbohydrate metabolism, Pentose shunt |
Ligand | Cobalt, Iron, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 5.1.3.1, 4460 |
PlantReactomei | R-OSA-1119519, Calvin cycle |
SABIO-RKi | Q9SE42 |
UniPathwayi | UPA00115;UER00411 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribulose-phosphate 3-epimerase, cytoplasmic isoform (EC:5.1.3.1By similarity)Alternative name(s): Cyt-RPEase Pentose-5-phosphate 3-epimerase Short name: PPE RPEcyt Ribulose-5-phosphate-epimerase |
Gene namesi | Ordered Locus Names:Os09g0505700, LOC_Os09g32810 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171590 | 1 – 228 | Ribulose-phosphate 3-epimerase, cytoplasmic isoformAdd BLAST | 228 |
Proteomic databases
PaxDbi | Q9SE42 |
PRIDEi | Q9SE42 |
Expressioni
Tissue specificityi
Predominantly accumulates in roots and seedlings.1 Publication
Gene expression databases
ExpressionAtlasi | Q9SE42, baseline and differential |
Genevisiblei | Q9SE42, OS |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
STRINGi | 4530.OS09T0505700-02 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9SE42 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9SE42 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 150 – 153 | Substrate bindingBy similarity | 4 | |
Regioni | 179 – 181 | Substrate bindingBy similarity | 3 | |
Regioni | 201 – 202 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the ribulose-phosphate 3-epimerase family.Curated
Phylogenomic databases
eggNOGi | KOG3111, Eukaryota |
HOGENOMi | CLU_054856_0_1_1 |
InParanoidi | Q9SE42 |
OMAi | ISVHYES |
OrthoDBi | 1554029at2759 |
Family and domain databases
CDDi | cd00429, RPE, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_02227, RPE, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR026019, Ribul_P_3_epim IPR000056, Ribul_P_3_epim-like IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR11749, PTHR11749, 1 hit |
Pfami | View protein in Pfam PF00834, Ribul_P_3_epim, 1 hit |
PIRSFi | PIRSF001461, RPE, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR01163, rpe, 1 hit |
PROSITEi | View protein in PROSITE PS01085, RIBUL_P_3_EPIMER_1, 1 hit PS01086, RIBUL_P_3_EPIMER_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9SE42-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAAAAAKIA PSMLSSDFAN LAAEADRMVR LGADWLHMDI MDGHFVPNLT
60 70 80 90 100
IGAPVIQSLR KHTKAYLDCH LMVTNPSDYV EPLAKAGASG FTFHIEVSRD
110 120 130 140 150
NWQELIQSIK AKGMRPGVSL RPGTPVEEVF PLVEAENPVE LVLVMTVEPG
160 170 180 190 200
FGGQKFMPEM MEKVRALRKK YPSLDIEVDG GLGPSTIDVA ASAGANCIVA
210 220
GSSIFGAAEP GEVISALRKS VEGSQNKS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0P0XQA9 | A0A0P0XQA9_ORYSJ | Os09g0505700 protein | Os09g0505700, OSNPB_090505700 | 205 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 – 43 | DG → GR in AC108753 (PubMed:16100779).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF189365 mRNA Translation: AAF01048.1 AC108753 Genomic DNA No translation available. AP008215 Genomic DNA Translation: BAF25518.2 AP014965 Genomic DNA Translation: BAT08853.1 AK069451 mRNA Translation: BAG91440.1 |
RefSeqi | XP_015611665.1, XM_015756179.1 |
Genome annotation databases
EnsemblPlantsi | Os09t0505700-02; Os09t0505700-02; Os09g0505700 |
GeneIDi | 4347508 |
Gramenei | Os09t0505700-02; Os09t0505700-02; Os09g0505700 |
KEGGi | osa:4347508 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF189365 mRNA Translation: AAF01048.1 AC108753 Genomic DNA No translation available. AP008215 Genomic DNA Translation: BAF25518.2 AP014965 Genomic DNA Translation: BAT08853.1 AK069451 mRNA Translation: BAG91440.1 |
RefSeqi | XP_015611665.1, XM_015756179.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H1Y | X-ray | 1.87 | A/B | 1-228 | [»] | |
1H1Z | X-ray | 3.40 | A/B | 1-228 | [»] | |
SMRi | Q9SE42 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 4530.OS09T0505700-02 |
Proteomic databases
PaxDbi | Q9SE42 |
PRIDEi | Q9SE42 |
Genome annotation databases
EnsemblPlantsi | Os09t0505700-02; Os09t0505700-02; Os09g0505700 |
GeneIDi | 4347508 |
Gramenei | Os09t0505700-02; Os09t0505700-02; Os09g0505700 |
KEGGi | osa:4347508 |
Phylogenomic databases
eggNOGi | KOG3111, Eukaryota |
HOGENOMi | CLU_054856_0_1_1 |
InParanoidi | Q9SE42 |
OMAi | ISVHYES |
OrthoDBi | 1554029at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00115;UER00411 |
BRENDAi | 5.1.3.1, 4460 |
PlantReactomei | R-OSA-1119519, Calvin cycle |
SABIO-RKi | Q9SE42 |
Miscellaneous databases
EvolutionaryTracei | Q9SE42 |
Gene expression databases
ExpressionAtlasi | Q9SE42, baseline and differential |
Genevisiblei | Q9SE42, OS |
Family and domain databases
CDDi | cd00429, RPE, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_02227, RPE, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR026019, Ribul_P_3_epim IPR000056, Ribul_P_3_epim-like IPR011060, RibuloseP-bd_barrel |
PANTHERi | PTHR11749, PTHR11749, 1 hit |
Pfami | View protein in Pfam PF00834, Ribul_P_3_epim, 1 hit |
PIRSFi | PIRSF001461, RPE, 1 hit |
SUPFAMi | SSF51366, SSF51366, 1 hit |
TIGRFAMsi | TIGR01163, rpe, 1 hit |
PROSITEi | View protein in PROSITE PS01085, RIBUL_P_3_EPIMER_1, 1 hit PS01086, RIBUL_P_3_EPIMER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPE1_ORYSJ | |
Accessioni | Q9SE42Primary (citable) accession number: Q9SE42 Secondary accession number(s): B7EGE3, Q0J0L5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | May 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families