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Protein

Flavonoid 3'-monooxygenase

Gene

CYP75B1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 3'-hydroxylation of the flavonoid B-ring to the 3',4'-hydroxylated state. Convert naringenin to eriodictyol and dihydrokaempferol to dihydroquercetin.

Miscellaneous

May act as a membrane anchor for localization of other, soluble, flavonoid enzymes to the endoplasmic reticulum.

Catalytic activityi

A flavonoid + [reduced NADPH--hemoprotein reductase] + O2 = a 3'-hydroxyflavonoid + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi445Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processFlavonoid biosynthesis
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:AT5G07990-MONOMER
BRENDAi1.14.13.21 399
UniPathwayiUPA00154

Names & Taxonomyi

Protein namesi
Recommended name:
Flavonoid 3'-monooxygenase (EC:1.14.14.82)
Alternative name(s):
Cytochrome P450 75B1
Flavonoid 3'-hydroxylase
Short name:
AtF3'H
Protein TRANSPARENT TESTA 7
Gene namesi
Name:CYP75B1
Synonyms:F3'H, TT7
Ordered Locus Names:At5g07990
ORF Names:F13G24.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G07990
TAIRilocus:2142878 AT5G07990

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Topological domaini22 – 513CytoplasmicSequence analysisAdd BLAST492

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521361 – 513Flavonoid 3'-monooxygenaseAdd BLAST513

Proteomic databases

PaxDbiQ9SD85

Expressioni

Tissue specificityi

High expression in siliques and to a lower extent in stems, flowers and senescing leaves.

Inductioni

By UV light treatment.

Gene expression databases

ExpressionAtlasiQ9SD85 baseline and differential
GenevisibleiQ9SD85 AT

Interactioni

Protein-protein interaction databases

BioGridi15971, 1 interactor
STRINGi3702.AT5G07990.1

Structurei

3D structure databases

ProteinModelPortaliQ9SD85
SMRiQ9SD85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOGENOMiHOG000218628
KOiK05280
OMAiRFTYEDT
OrthoDBiEOG0936097G
PhylomeDBiQ9SD85

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SD85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLFLTILL ATVLFLILRI FSHRRNRSHN NRLPPGPNPW PIIGNLPHMG
60 70 80 90 100
TKPHRTLSAM VTTYGPILHL RLGFVDVVVA ASKSVAEQFL KIHDANFASR
110 120 130 140 150
PPNSGAKHMA YNYQDLVFAP YGHRWRLLRK ISSVHLFSAK ALEDFKHVRQ
160 170 180 190 200
EEVGTLTREL VRVGTKPVNL GQLVNMCVVN ALGREMIGRR LFGADADHKA
210 220 230 240 250
DEFRSMVTEM MALAGVFNIG DFVPSLDWLD LQGVAGKMKR LHKRFDAFLS
260 270 280 290 300
SILKEHEMNG QDQKHTDMLS TLISLKGTDL DGDGGSLTDT EIKALLLNMF
310 320 330 340 350
TAGTDTSAST VDWAIAELIR HPDIMVKAQE ELDIVVGRDR PVNESDIAQL
360 370 380 390 400
PYLQAVIKEN FRLHPPTPLS LPHIASESCE INGYHIPKGS TLLTNIWAIA
410 420 430 440 450
RDPDQWSDPL AFKPERFLPG GEKSGVDVKG SDFELIPFGA GRRICAGLSL
460 470 480 490 500
GLRTIQFLTA TLVQGFDWEL AGGVTPEKLN MEESYGLTLQ RAVPLVVHPK
510
PRLAPNVYGL GSG
Length:513
Mass (Da):56,787
Last modified:May 1, 2000 - v1
Checksum:iC0C740FBE4559C40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271651 mRNA Translation: AAG16746.1
AF271650 mRNA Translation: AAG16745.1
AF155171 Genomic DNA Translation: AAF73253.1
AH009204 Genomic DNA Translation: AAF60189.1
AL133421 Genomic DNA Translation: CAB62611.1
CP002688 Genomic DNA Translation: AED91232.1
PIRiT45624
RefSeqiNP_196416.1, NM_120881.3
UniGeneiAt.12059

Genome annotation databases

EnsemblPlantsiAT5G07990.1; AT5G07990.1; AT5G07990
GeneIDi830693
GrameneiAT5G07990.1; AT5G07990.1; AT5G07990
KEGGiath:AT5G07990

Similar proteinsi

Entry informationi

Entry nameiF3PH_ARATH
AccessioniPrimary (citable) accession number: Q9SD85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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