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UniProtKB - Q9SD85 (F3PH_ARATH)
Protein
Flavonoid 3'-monooxygenase
Gene
CYP75B1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the 3'-hydroxylation of the flavonoid B-ring to the 3',4'-hydroxylated state. Convert naringenin to eriodictyol and dihydrokaempferol to dihydroquercetin.
Miscellaneous
May act as a membrane anchor for localization of other, soluble, flavonoid enzymes to the endoplasmic reticulum.
Catalytic activityi
- a 3'-unsubstituted flavone + O2 + reduced [NADPH—hemoprotein reductase] = a 3'-hydroxyflavone + H+ + H2O + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.82
Cofactori
hemeBy similarity
: flavonoid biosynthesis Pathwayi
This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 445 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- flavonoid 3'-monooxygenase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: GO_Central
GO - Biological processi
- flavonoid biosynthetic process Source: UniProtKB-UniPathway
- response to auxin Source: TAIR
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Flavonoid biosynthesis |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
BRENDAi | 1.14.14.82, 399 |
UniPathwayi | UPA00154 |
Names & Taxonomyi
Protein namesi | Recommended name: Flavonoid 3'-monooxygenase (EC:1.14.14.82)Alternative name(s): Cytochrome P450 75B1 Flavonoid 3'-hydroxylase Short name: AtF3'H Protein TRANSPARENT TESTA 7 |
Gene namesi | Name:CYP75B1 Synonyms:F3'H, TT7 Ordered Locus Names:At5g07990 ORF Names:F13G24.190 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G07990 |
TAIRi | locus:2142878, AT5G07990 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Single-pass type III membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 21 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 22 – 513 | CytoplasmicSequence analysisAdd BLAST | 492 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000052136 | 1 – 513 | Flavonoid 3'-monooxygenaseAdd BLAST | 513 |
Proteomic databases
PaxDbi | Q9SD85 |
ProteomicsDBi | 222359 |
Expressioni
Tissue specificityi
High expression in siliques and to a lower extent in stems, flowers and senescing leaves.
Inductioni
By UV light treatment.
Gene expression databases
ExpressionAtlasi | Q9SD85, baseline and differential |
Genevisiblei | Q9SD85, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 15971, 1 interactor |
STRINGi | 3702.AT5G07990.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0156, Eukaryota |
HOGENOMi | CLU_001570_4_0_1 |
OMAi | WATYEDR |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q9SD85 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9SD85-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATLFLTILL ATVLFLILRI FSHRRNRSHN NRLPPGPNPW PIIGNLPHMG
60 70 80 90 100
TKPHRTLSAM VTTYGPILHL RLGFVDVVVA ASKSVAEQFL KIHDANFASR
110 120 130 140 150
PPNSGAKHMA YNYQDLVFAP YGHRWRLLRK ISSVHLFSAK ALEDFKHVRQ
160 170 180 190 200
EEVGTLTREL VRVGTKPVNL GQLVNMCVVN ALGREMIGRR LFGADADHKA
210 220 230 240 250
DEFRSMVTEM MALAGVFNIG DFVPSLDWLD LQGVAGKMKR LHKRFDAFLS
260 270 280 290 300
SILKEHEMNG QDQKHTDMLS TLISLKGTDL DGDGGSLTDT EIKALLLNMF
310 320 330 340 350
TAGTDTSAST VDWAIAELIR HPDIMVKAQE ELDIVVGRDR PVNESDIAQL
360 370 380 390 400
PYLQAVIKEN FRLHPPTPLS LPHIASESCE INGYHIPKGS TLLTNIWAIA
410 420 430 440 450
RDPDQWSDPL AFKPERFLPG GEKSGVDVKG SDFELIPFGA GRRICAGLSL
460 470 480 490 500
GLRTIQFLTA TLVQGFDWEL AGGVTPEKLN MEESYGLTLQ RAVPLVVHPK
510
PRLAPNVYGL GSG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF271651 mRNA Translation: AAG16746.1 AF271650 mRNA Translation: AAG16745.1 AF155171 Genomic DNA Translation: AAF73253.1 AH009204 Genomic DNA Translation: AAF60189.1 AL133421 Genomic DNA Translation: CAB62611.1 CP002688 Genomic DNA Translation: AED91232.1 |
PIRi | T45624 |
RefSeqi | NP_196416.1, NM_120881.3 |
Genome annotation databases
EnsemblPlantsi | AT5G07990.1; AT5G07990.1; AT5G07990 |
GeneIDi | 830693 |
Gramenei | AT5G07990.1; AT5G07990.1; AT5G07990 |
KEGGi | ath:AT5G07990 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF271651 mRNA Translation: AAG16746.1 AF271650 mRNA Translation: AAG16745.1 AF155171 Genomic DNA Translation: AAF73253.1 AH009204 Genomic DNA Translation: AAF60189.1 AL133421 Genomic DNA Translation: CAB62611.1 CP002688 Genomic DNA Translation: AED91232.1 |
PIRi | T45624 |
RefSeqi | NP_196416.1, NM_120881.3 |
3D structure databases
SMRi | Q9SD85 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 15971, 1 interactor |
STRINGi | 3702.AT5G07990.1 |
Proteomic databases
PaxDbi | Q9SD85 |
ProteomicsDBi | 222359 |
Genome annotation databases
EnsemblPlantsi | AT5G07990.1; AT5G07990.1; AT5G07990 |
GeneIDi | 830693 |
Gramenei | AT5G07990.1; AT5G07990.1; AT5G07990 |
KEGGi | ath:AT5G07990 |
Organism-specific databases
Araporti | AT5G07990 |
TAIRi | locus:2142878, AT5G07990 |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
HOGENOMi | CLU_001570_4_0_1 |
OMAi | WATYEDR |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q9SD85 |
Enzyme and pathway databases
UniPathwayi | UPA00154 |
BRENDAi | 1.14.14.82, 399 |
Miscellaneous databases
PROi | PR:Q9SD85 |
Gene expression databases
ExpressionAtlasi | Q9SD85, baseline and differential |
Genevisiblei | Q9SD85, AT |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | F3PH_ARATH | |
Accessioni | Q9SD85Primary (citable) accession number: Q9SD85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 13, 2002 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families