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UniProtKB - Q9SCX9 (GPDA1_ARATH)
Protein
Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic
Gene
DHAPRD
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in glycerolipid metabolism.
By similarityCatalytic activityi
- EC:1.1.1.8
Kineticsi
- KM=12.8 µM for glycerone phosphate1 Publication
- Vmax=3.2 µmol/sec/mg enzyme toward glycerone phosphate1 Publication
: glycerophospholipid metabolism Pathwayi
This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 92 | NADBy similarity | 1 | |
Binding sitei | 149 | NADBy similarity | 1 | |
Binding sitei | 172 | NAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 172 | SubstrateBy similarity | 1 | |
Binding sitei | 205 | NAD; via amide nitrogenBy similarity | 1 | |
Active sitei | 257 | Proton acceptorBy similarity | 1 | |
Binding sitei | 321 | NADBy similarity | 1 | |
Binding sitei | 350 | NAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 352 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 61 – 66 | NADBy similarity | 6 |
GO - Molecular functioni
- glycerol-3-phosphate dehydrogenase [NAD+] activity Source: GO_Central
- NAD binding Source: InterPro
- protein homodimerization activity Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- glycerol-3-phosphate catabolic process Source: InterPro
- glycerol-3-phosphate metabolic process Source: GO_Central
- glycerophospholipid metabolic process Source: UniProtKB-UniPathway
- NADH oxidation Source: GO_Central
- phospholipid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | NAD |
Enzyme and pathway databases
UniPathwayi | UPA00940 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DHAPRD Ordered Locus Names:At5g40610 ORF Names:MNF13.16 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G40610 |
TAIRi | locus:2168758, AT5G40610 |
Subcellular locationi
Chloroplast
- chloroplast 1 Publication
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Cytosol
- cytosol Source: GO_Central
Other locations
- glycerol-3-phosphate dehydrogenase complex Source: InterPro
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 32 | ChloroplastSequence analysisAdd BLAST | 32 | |
ChainiPRO_0000287871 | 33 – 400 | Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplasticAdd BLAST | 368 |
Proteomic databases
PaxDbi | Q9SCX9 |
PRIDEi | Q9SCX9 |
ProteomicsDBi | 248463 |
Expressioni
Tissue specificityi
Expressed in young seedlings, flowers and siliques. Expressed at low levels in roots.1 Publication
Gene expression databases
ExpressionAtlasi | Q9SCX9, baseline and differential |
Genevisiblei | Q9SCX9, AT |
Interactioni
GO - Molecular functioni
- protein homodimerization activity Source: InterPro
Protein-protein interaction databases
STRINGi | 3702.AT5G40610.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 321 – 322 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2711, Eukaryota |
HOGENOMi | CLU_033449_2_2_1 |
InParanoidi | Q9SCX9 |
OMAi | FIHKVCD |
OrthoDBi | 476066at2759 |
PhylomeDBi | Q9SCX9 |
Family and domain databases
Gene3Di | 1.10.1040.10, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR006168, G3P_DH_NAD-dep IPR006109, G3P_DH_NAD-dep_C IPR017751, G3P_DH_NAD-dep_euk IPR011128, G3P_DH_NAD-dep_N IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF07479, NAD_Gly3P_dh_C, 1 hit PF01210, NAD_Gly3P_dh_N, 1 hit |
PIRSFi | PIRSF000114, Glycerol-3-P_dh, 1 hit |
PRINTSi | PR00077, GPDHDRGNASE |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR03376, glycerol3P_DH, 1 hit |
PROSITEi | View protein in PROSITE PS00957, NAD_G3PDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9SCX9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRFRSFFFSS SIFSLSHSRS PSLSSSRFSS LSAAMSPALE KSRQGNGGCN
60 70 80 90 100
DDSKSKVTVV GSGNWGSVAA KLIASNALKL PSFHDEVRMW VFEEVLPNGE
110 120 130 140 150
KLNDVINKTN ENVKYLPGIK LGRNVVADPD LENAVKDANM LVFVTPHQFM
160 170 180 190 200
DGICKKLDGK ITGDVEAISL VKGMEVKKEG PCMISSLISK QLGINCCVLM
210 220 230 240 250
GANIANEIAV EKFSEATVGY RGSREIADTW VQLFSTPYFM VTPVHDVEGV
260 270 280 290 300
ELCGTLKNVV AIAAGFVDGL EMGNNTKAAI MRIGLREMKA LSKLLFPSVK
310 320 330 340 350
DSTFFESCGV ADVITTCLGG RNRRVAEAFA KSRGKRSFDE LEAEMLQGQK
360 370 380 390 400
LQGVSTAREV YEVLKHCGWL EMFPLFSTVH QICTGRLQPE AIVQYRENKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ242602 mRNA Translation: CAB64726.1 AB009052 Genomic DNA Translation: BAB08532.1 CP002688 Genomic DNA Translation: AED94573.1 BT000967 mRNA Translation: AAN41367.1 AY080863 mRNA Translation: AAL87336.1 |
RefSeqi | NP_198877.1, NM_123425.5 |
Genome annotation databases
EnsemblPlantsi | AT5G40610.1; AT5G40610.1; AT5G40610 |
GeneIDi | 834060 |
Gramenei | AT5G40610.1; AT5G40610.1; AT5G40610 |
KEGGi | ath:AT5G40610 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ242602 mRNA Translation: CAB64726.1 AB009052 Genomic DNA Translation: BAB08532.1 CP002688 Genomic DNA Translation: AED94573.1 BT000967 mRNA Translation: AAN41367.1 AY080863 mRNA Translation: AAL87336.1 |
RefSeqi | NP_198877.1, NM_123425.5 |
3D structure databases
AlphaFoldDBi | Q9SCX9 |
SMRi | Q9SCX9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G40610.1 |
Proteomic databases
PaxDbi | Q9SCX9 |
PRIDEi | Q9SCX9 |
ProteomicsDBi | 248463 |
Genome annotation databases
EnsemblPlantsi | AT5G40610.1; AT5G40610.1; AT5G40610 |
GeneIDi | 834060 |
Gramenei | AT5G40610.1; AT5G40610.1; AT5G40610 |
KEGGi | ath:AT5G40610 |
Organism-specific databases
Araporti | AT5G40610 |
TAIRi | locus:2168758, AT5G40610 |
Phylogenomic databases
eggNOGi | KOG2711, Eukaryota |
HOGENOMi | CLU_033449_2_2_1 |
InParanoidi | Q9SCX9 |
OMAi | FIHKVCD |
OrthoDBi | 476066at2759 |
PhylomeDBi | Q9SCX9 |
Enzyme and pathway databases
UniPathwayi | UPA00940 |
Miscellaneous databases
PROi | PR:Q9SCX9 |
Gene expression databases
ExpressionAtlasi | Q9SCX9, baseline and differential |
Genevisiblei | Q9SCX9, AT |
Family and domain databases
Gene3Di | 1.10.1040.10, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR006168, G3P_DH_NAD-dep IPR006109, G3P_DH_NAD-dep_C IPR017751, G3P_DH_NAD-dep_euk IPR011128, G3P_DH_NAD-dep_N IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF07479, NAD_Gly3P_dh_C, 1 hit PF01210, NAD_Gly3P_dh_N, 1 hit |
PIRSFi | PIRSF000114, Glycerol-3-P_dh, 1 hit |
PRINTSi | PR00077, GPDHDRGNASE |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR03376, glycerol3P_DH, 1 hit |
PROSITEi | View protein in PROSITE PS00957, NAD_G3PDH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GPDA1_ARATH | |
Accessioni | Q9SCX9Primary (citable) accession number: Q9SCX9 Secondary accession number(s): Q8RXI6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families