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Protein

Protein S-acyltransferase 8

Gene

PAT08

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-acyltransferase involved in protein lipid modification.2 Publications

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225 399

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S-acyltransferase 8 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At4g24630
Zinc finger DHHC domain-containing protein At4g24630
Gene namesi
Name:PAT08
Ordered Locus Names:At4g24630
ORF Names:F22K18.170
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G24630
TAIRilocus:2121949 AT4G24630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003120301 – 407Protein S-acyltransferase 8Add BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9SB58
PRIDEiQ9SB58

PTM databases

iPTMnetiQ9SB58
SwissPalmiQ9SB58

Expressioni

Tissue specificityi

Expressed in flowers and pollen.1 Publication

Gene expression databases

ExpressionAtlasiQ9SB58 baseline and differential
GenevisibleiQ9SB58 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G24630.1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 186DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1311 Eukaryota
COG5273 LUCA
HOGENOMiHOG000242943
InParanoidiQ9SB58
KOiK16675
OMAiIRTEARH
OrthoDBiEOG09360EBP
PhylomeDBiQ9SB58

Family and domain databases

InterProiView protein in InterPro
IPR001594 Palmitoyltrfase_DHHC
PfamiView protein in Pfam
PF01529 DHHC, 1 hit
PROSITEiView protein in PROSITE
PS50216 DHHC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9SB58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQRVFQVWK GSNKFILGGR LIFGPDARSL PLTLLLIIVP VVLFCVFVAR
60 70 80 90 100
HLRHEFSPYN AGYAIMVVAI LFTIYVLILL FFTSARDPGI VPRNSHPPEE
110 120 130 140 150
DLRYETTVSA DGRQTPSVQI PRTKEVIVNG VSVRVKYCDT CMLYRPPRCS
160 170 180 190 200
HCSICNNCVE RFDHHCPWVG QCIGLRNYRY FFMFVSSSTL LCIYIFSMSA
210 220 230 240 250
VYIKILMDHQ QATVWRAMKE SPWAVVLMIY CFIALWFVGG LTAFHLYLIS
260 270 280 290 300
TNQTTYEKLR YRSSHSRSIV YNRGCPNNFL EVFCSKVKPS RNNFRAFIEE
310 320 330 340 350
EPPRVITLPS TTRESGEAED ENVTRRQKVE DDLDIGDDLM NLSRRCNAED
360 370 380 390 400
ANNNQPHHTL DIDHERAGSI RTEARHESWG RRSGSWDVAA TDVRESRSYA

TAKDGRG
Length:407
Mass (Da):46,747
Last modified:December 4, 2007 - v2
Checksum:i523003A85C366340
GO

Sequence cautioni

The sequence CAA23000 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79373 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035356 Genomic DNA Translation: CAA23000.1 Sequence problems.
AL161561 Genomic DNA Translation: CAB79373.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE84935.1
AK226186 mRNA Translation: BAE98351.1
PIRiT05571
RefSeqiNP_194194.2, NM_118596.3
UniGeneiAt.54503

Genome annotation databases

EnsemblPlantsiAT4G24630.1; AT4G24630.1; AT4G24630
GeneIDi828565
GrameneiAT4G24630.1; AT4G24630.1; AT4G24630
KEGGiath:AT4G24630

Similar proteinsi

Entry informationi

Entry nameiZDH19_ARATH
AccessioniPrimary (citable) accession number: Q9SB58
Secondary accession number(s): Q0WWZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: April 25, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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