UniProtKB - Q9SB48 (NCPR1_ARATH)
NADPH--cytochrome P450 reductase 1
ATR1
Functioni
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and dichloroindophenol.
UniRule annotation5 PublicationsCatalytic activityi
- EC:1.6.2.4UniRule annotation
Cofactori
Protein has several cofactor binding sites:- FADUniRule annotation1 PublicationNote: Binds 1 FAD per monomer.UniRule annotation
- FMNUniRule annotation1 PublicationNote: Binds 1 FMN per monomer.UniRule annotation
Kineticsi
- KM=2.2 µM for NADPH (at pH 7.0 and 25 degrees Celsius)3 Publications
- KM=21.9 µM for NADPH (at pH 7.7 and 28 degrees Celsius)3 Publications
- KM=2.0 µM for NADPH (at pH 7.25 and 25 degrees Celsius)3 Publications
- KM=17 µM for cytochrome c (at pH 7.0 and 25 degrees Celsius)3 Publications
- KM=24.3 µM for cytochrome c (at pH 7.7 and 28 degrees Celsius)3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 219 | FMNUniRule annotation | 1 | |
Binding sitei | 310 | NADPUniRule annotation | 1 | |
Binding sitei | 551 | NADPUniRule annotation | 1 | |
Binding sitei | 654 | NADPUniRule annotation | 1 | |
Binding sitei | 692 | FADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 91 – 96 | FMNUniRule annotation | 6 | |
Nucleotide bindingi | 146 – 149 | FMNUniRule annotation | 4 | |
Nucleotide bindingi | 184 – 193 | FMNUniRule annotation | 10 | |
Nucleotide bindingi | 470 – 473 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 488 – 490 | FADUniRule annotation | 3 | |
Nucleotide bindingi | 504 – 507 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 612 – 613 | NADPUniRule annotation | 2 | |
Nucleotide bindingi | 618 – 622 | NADPUniRule annotation | 5 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: GO_Central
- FMN binding Source: GO_Central
- NADP binding Source: UniProtKB-UniRule
- NADPH-hemoprotein reductase activity Source: TAIR
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- phenylpropanoid metabolic process Source: TAIR
- response to oxidative stress Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Phenylpropanoid metabolism |
Ligand | FAD, Flavoprotein, FMN, NADP |
Enzyme and pathway databases
BioCyci | ARA:AT4G24520-MONOMER |
BRENDAi | 1.6.2.4, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH--cytochrome P450 reductase 1UniRule annotation (EC:1.6.2.4UniRule annotation)Short name: AtCPR11 Publication Short name: CPR 12 PublicationsUniRule annotation Short name: P450R 1UniRule annotation Alternative name(s): Cytochrome P450 73 A51 Publication |
Gene namesi | Name:ATR12 Publications Synonyms:AR11 Publication, CYP73A51 Publication Ordered Locus Names:At4g24520Imported ORF Names:F22K18.280Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G24520 |
TAIRi | locus:2121894, AT4G24520 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Cytosol
- cytosol Source: TAIR
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 26 | LumenalUniRule annotationAdd BLAST | 25 | |
Transmembranei | 27 – 47 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 48 – 692 | CytoplasmicUniRule annotationAdd BLAST | 645 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Biotechnological usei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000416839 | 2 – 692 | NADPH--cytochrome P450 reductase 1Add BLAST | 691 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineCombined sources | 1 | |
Modified residuei | 249 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q9SB48 |
ProteomicsDBi | 251284 [Q9SB48-1] |
PTM databases
iPTMneti | Q9SB48 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q9SB48, baseline and differential |
Genevisiblei | Q9SB48, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 13843, 2 interactors |
STRINGi | 3702.AT4G24520.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 85 – 235 | Flavodoxin-likeUniRule annotationAdd BLAST | 151 | |
Domaini | 290 – 537 | FAD-binding FR-typeUniRule annotationAdd BLAST | 248 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1158, Eukaryota |
HOGENOMi | CLU_001570_17_3_1 |
InParanoidi | Q9SB48 |
OMAi | WDKPDPY |
PhylomeDBi | Q9SB48 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212, NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR023208, P450R IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208, P450R, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSALYASDL FKQLKSIMGT DSLSDDVVLV IATTSLALVA GFVVLLWKKT
60 70 80 90 100
TADRSGELKP LMIPKSLMAK DEDDDLDLGS GKTRVSIFFG TQTGTAEGFA
110 120 130 140 150
KALSEEIKAR YEKAAVKVID LDDYAADDDQ YEEKLKKETL AFFCVATYGD
160 170 180 190 200
GEPTDNAARF YKWFTEENER DIKLQQLAYG VFALGNRQYE HFNKIGIVLD
210 220 230 240 250
EELCKKGAKR LIEVGLGDDD QSIEDDFNAW KESLWSELDK LLKDEDDKSV
260 270 280 290 300
ATPYTAVIPE YRVVTHDPRF TTQKSMESNV ANGNTTIDIH HPCRVDVAVQ
310 320 330 340 350
KELHTHESDR SCIHLEFDIS RTGITYETGD HVGVYAENHV EIVEEAGKLL
360 370 380 390 400
GHSLDLVFSI HADKEDGSPL ESAVPPPFPG PCTLGTGLAR YADLLNPPRK
410 420 430 440 450
SALVALAAYA TEPSEAEKLK HLTSPDGKDE YSQWIVASQR SLLEVMAAFP
460 470 480 490 500
SAKPPLGVFF AAIAPRLQPR YYSISSSPRL APSRVHVTSA LVYGPTPTGR
510 520 530 540 550
IHKGVCSTWM KNAVPAEKSH ECSGAPIFIR ASNFKLPSNP STPIVMVGPG
560 570 580 590 600
TGLAPFRGFL QERMALKEDG EELGSSLLFF GCRNRQMDFI YEDELNNFVD
610 620 630 640 650
QGVISELIMA FSREGAQKEY VQHKMMEKAA QVWDLIKEEG YLYVCGDAKG
660 670 680 690
MARDVHRTLH TIVQEQEGVS SSEAEAIVKK LQTEGRYLRD VW
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4JQY4 | F4JQY4_ARATH | NADPH--cytochrome P450 reductase | ATR1 AR1, P450 reductase 1, At4g24520, F22K18.280, F22K18_280 | 688 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 161 | Y → S in CAA46814 (PubMed:9235908).Curated | 1 | |
Sequence conflicti | 477 – 480 | SPRL → CQDW in CAA46814 (PubMed:9235908).Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66016 mRNA Translation: CAA46814.1 AL035356 Genomic DNA Translation: CAA23011.1 AL161561 Genomic DNA Translation: CAB79362.1 CP002687 Genomic DNA Translation: AEE84919.1 AY054688 mRNA Translation: AAK96879.1 BT008426 mRNA Translation: AAP37785.1 |
PIRi | T05582 |
RefSeqi | NP_194183.1, NM_118585.4 [Q9SB48-1] |
Genome annotation databases
EnsemblPlantsi | AT4G24520.1; AT4G24520.1; AT4G24520 [Q9SB48-1] |
GeneIDi | 828554 |
Gramenei | AT4G24520.1; AT4G24520.1; AT4G24520 [Q9SB48-1] |
KEGGi | ath:AT4G24520 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66016 mRNA Translation: CAA46814.1 AL035356 Genomic DNA Translation: CAA23011.1 AL161561 Genomic DNA Translation: CAB79362.1 CP002687 Genomic DNA Translation: AEE84919.1 AY054688 mRNA Translation: AAK96879.1 BT008426 mRNA Translation: AAP37785.1 |
PIRi | T05582 |
RefSeqi | NP_194183.1, NM_118585.4 [Q9SB48-1] |
3D structure databases
SMRi | Q9SB48 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 13843, 2 interactors |
STRINGi | 3702.AT4G24520.1 |
PTM databases
iPTMneti | Q9SB48 |
Proteomic databases
PaxDbi | Q9SB48 |
ProteomicsDBi | 251284 [Q9SB48-1] |
Genome annotation databases
EnsemblPlantsi | AT4G24520.1; AT4G24520.1; AT4G24520 [Q9SB48-1] |
GeneIDi | 828554 |
Gramenei | AT4G24520.1; AT4G24520.1; AT4G24520 [Q9SB48-1] |
KEGGi | ath:AT4G24520 |
Organism-specific databases
Araporti | AT4G24520 |
TAIRi | locus:2121894, AT4G24520 |
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
HOGENOMi | CLU_001570_17_3_1 |
InParanoidi | Q9SB48 |
OMAi | WDKPDPY |
PhylomeDBi | Q9SB48 |
Enzyme and pathway databases
BioCyci | ARA:AT4G24520-MONOMER |
BRENDAi | 1.6.2.4, 399 |
Miscellaneous databases
PROi | PR:Q9SB48 |
Gene expression databases
ExpressionAtlasi | Q9SB48, baseline and differential |
Genevisiblei | Q9SB48, AT |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212, NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR023208, P450R IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208, P450R, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NCPR1_ARATH | |
Accessioni | Q9SB48Primary (citable) accession number: Q9SB48 Secondary accession number(s): Q39035 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 18, 2012 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families