UniProtKB - Q9SAJ6 (G3PP1_ARATH)
Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplastic
GAPCP1
Functioni
Involved in plastidial glycolytic pathway and plays a specific role in glycolytic energy production in non-green plastids and chloroplasts. Essential for breakdown of starch to form sucrose for export to non-photosynthetic tissues, and to generate primary metabolites for anabolic pathways such as fatty acid and amino acid synthesis. Plays an important role in plant development by providing substrates for the phosphorylated pathway of serine biosynthesis in roots. Plays a crucial role in pollen development. Functionally redundant with GAPCP2.
2 PublicationsMiscellaneous
Catalytic activityi
- D-glyceraldehyde 3-phosphate + NAD+ + phosphate = (2R)-3-phospho-glyceroyl phosphate + H+ + NADHPROSITE-ProRule annotationEC:1.2.1.12PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | NADBy similarity | 1 | |
Binding sitei | 164 | NAD; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 236 | NucleophilePROSITE-ProRule annotation | 1 | |
Sitei | 263 | Activates thiol group during catalysisBy similarity | 1 | |
Binding sitei | 266 | Glyceraldehyde 3-phosphateBy similarity | 1 | |
Binding sitei | 318 | Glyceraldehyde 3-phosphateBy similarity | 1 | |
Binding sitei | 400 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 96 – 97 | NADBy similarity | 2 |
GO - Molecular functioni
- copper ion binding Source: TAIR
- glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: TAIR
- NAD binding Source: InterPro
- NADP binding Source: InterPro
- zinc ion binding Source: TAIR
GO - Biological processi
- amino acid homeostasis Source: TAIR
- anther wall tapetum development Source: TAIR
- carbohydrate metabolic process Source: TAIR
- glucose metabolic process Source: InterPro
- glycolytic process Source: GO_Central
- pollen development Source: TAIR
- primary root development Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glycolysis |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.2.1.12, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplastic (EC:1.2.1.12)Alternative name(s): Glyceraldehyde-3-phosphate dehydrogenase of plastid 1 NAD-dependent glyceraldehydephosphate dehydrogenase chloroplastic 1 |
Gene namesi | Name:GAPCP1 Ordered Locus Names:At1g79530 ORF Names:T8K14.5 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G79530 |
TAIRi | locus:2206435, AT1G79530 |
Subcellular locationi
Chloroplast
- chloroplast stroma 1 Publication
Chloroplast
- chloroplast stroma Source: UniProtKB-SubCell
Cytosol
- cytosol Source: GO_Central
Other locations
- plastid Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 236 | C → G: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 263 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 311 | K → A: No effect on the activity. 1 Publication | 1 | |
Mutagenesisi | 318 | R → E: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 69 | ChloroplastCombined sourcesAdd BLAST | 69 | |
ChainiPRO_0000422407 | 70 – 422 | Glyceraldehyde-3-phosphate dehydrogenase GAPCP1, chloroplasticAdd BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 70 | N-acetylthreonineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q9SAJ6 |
PRIDEi | Q9SAJ6 |
ProMEXi | Q9SAJ6 |
ProteomicsDBi | 230442 |
PTM databases
iPTMneti | Q9SAJ6 |
SwissPalmi | Q9SAJ6 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
ExpressionAtlasi | Q9SAJ6, baseline and differential |
Genevisiblei | Q9SAJ6, AT |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
BioGRIDi | 29510, 2 interactors |
IntActi | Q9SAJ6, 2 interactors |
STRINGi | 3702.AT1G79530.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 50 – 84 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 235 – 237 | Glyceraldehyde 3-phosphate bindingBy similarity | 3 | |
Regioni | 295 – 296 | Glyceraldehyde 3-phosphate bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0657, Eukaryota |
HOGENOMi | CLU_030140_0_3_1 |
InParanoidi | Q9SAJ6 |
OMAi | FMKFVSW |
OrthoDBi | 945145at2759 |
PhylomeDBi | Q9SAJ6 |
Family and domain databases
InterProi | View protein in InterPro IPR020831, GlycerAld/Erythrose_P_DH IPR020830, GlycerAld_3-P_DH_AS IPR020829, GlycerAld_3-P_DH_cat IPR020828, GlycerAld_3-P_DH_NAD(P)-bd IPR006424, Glyceraldehyde-3-P_DH_1 IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR10836, PTHR10836, 1 hit |
Pfami | View protein in Pfam PF02800, Gp_dh_C, 1 hit PF00044, Gp_dh_N, 1 hit |
PRINTSi | PR00078, G3PDHDRGNASE |
SMARTi | View protein in SMART SM00846, Gp_dh_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01534, GAPDH-I, 1 hit |
PROSITEi | View protein in PROSITE PS00071, GAPDH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAFSSLLRSA ASYTVAAPRP DFFSSPASDH SKVLSSLGFS RNLKPSRFSS
60 70 80 90 100
GISSSLQNGN ARSVQPIKAT ATEVPSAVRR SSSSGKTKVG INGFGRIGRL
110 120 130 140 150
VLRIATSRDD IEVVAVNDPF IDAKYMAYML KYDSTHGNFK GSINVIDDST
160 170 180 190 200
LEINGKKVNV VSKRDPSEIP WADLGADYVV ESSGVFTTLS KAASHLKGGA
210 220 230 240 250
KKVIISAPSA DAPMFVVGVN EHTYQPNMDI VSNASCTTNC LAPLAKVVHE
260 270 280 290 300
EFGILEGLMT TVHATTATQK TVDGPSMKDW RGGRGASQNI IPSSTGAAKA
310 320 330 340 350
VGKVLPELNG KLTGMAFRVP TSNVSVVDLT CRLEKGASYE DVKAAIKHAS
360 370 380 390 400
EGPLKGILGY TDEDVVSNDF VGDSRSSIFD ANAGIGLSKS FVKLVSWYDN
410 420
EWGYSNRVLD LIEHMALVAA SH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007202 Genomic DNA Translation: AAD30223.1 CP002684 Genomic DNA Translation: AEE36260.1 AK117920 mRNA Translation: BAC42558.1 AF348583 mRNA Translation: AAK15554.1 |
PIRi | F96826 |
RefSeqi | NP_178071.1, NM_106601.4 |
Genome annotation databases
EnsemblPlantsi | AT1G79530.1; AT1G79530.1; AT1G79530 |
GeneIDi | 844291 |
Gramenei | AT1G79530.1; AT1G79530.1; AT1G79530 |
KEGGi | ath:AT1G79530 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007202 Genomic DNA Translation: AAD30223.1 CP002684 Genomic DNA Translation: AEE36260.1 AK117920 mRNA Translation: BAC42558.1 AF348583 mRNA Translation: AAK15554.1 |
PIRi | F96826 |
RefSeqi | NP_178071.1, NM_106601.4 |
3D structure databases
AlphaFoldDBi | Q9SAJ6 |
SMRi | Q9SAJ6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 29510, 2 interactors |
IntActi | Q9SAJ6, 2 interactors |
STRINGi | 3702.AT1G79530.1 |
PTM databases
iPTMneti | Q9SAJ6 |
SwissPalmi | Q9SAJ6 |
Proteomic databases
PaxDbi | Q9SAJ6 |
PRIDEi | Q9SAJ6 |
ProMEXi | Q9SAJ6 |
ProteomicsDBi | 230442 |
Genome annotation databases
EnsemblPlantsi | AT1G79530.1; AT1G79530.1; AT1G79530 |
GeneIDi | 844291 |
Gramenei | AT1G79530.1; AT1G79530.1; AT1G79530 |
KEGGi | ath:AT1G79530 |
Organism-specific databases
Araporti | AT1G79530 |
TAIRi | locus:2206435, AT1G79530 |
Phylogenomic databases
eggNOGi | KOG0657, Eukaryota |
HOGENOMi | CLU_030140_0_3_1 |
InParanoidi | Q9SAJ6 |
OMAi | FMKFVSW |
OrthoDBi | 945145at2759 |
PhylomeDBi | Q9SAJ6 |
Enzyme and pathway databases
BRENDAi | 1.2.1.12, 399 |
Miscellaneous databases
PROi | PR:Q9SAJ6 |
Gene expression databases
ExpressionAtlasi | Q9SAJ6, baseline and differential |
Genevisiblei | Q9SAJ6, AT |
Family and domain databases
InterProi | View protein in InterPro IPR020831, GlycerAld/Erythrose_P_DH IPR020830, GlycerAld_3-P_DH_AS IPR020829, GlycerAld_3-P_DH_cat IPR020828, GlycerAld_3-P_DH_NAD(P)-bd IPR006424, Glyceraldehyde-3-P_DH_1 IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR10836, PTHR10836, 1 hit |
Pfami | View protein in Pfam PF02800, Gp_dh_C, 1 hit PF00044, Gp_dh_N, 1 hit |
PRINTSi | PR00078, G3PDHDRGNASE |
SMARTi | View protein in SMART SM00846, Gp_dh_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01534, GAPDH-I, 1 hit |
PROSITEi | View protein in PROSITE PS00071, GAPDH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | G3PP1_ARATH | |
Accessioni | Q9SAJ6Primary (citable) accession number: Q9SAJ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2013 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families