UniProtKB - Q9S9T7 (VP282_ARATH)
Protein
Vacuolar protein sorting-associated protein 28 homolog 2
Gene
VPS28-2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex (By similarity).By similarity
GO - Molecular functioni
- protein-containing complex binding Source: GO_Central
GO - Biological processi
- protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Protein family/group databases
TCDBi | 3.A.31.1.2, the endosomal sorting complexes required for transport iii (escrt-iii) family |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 28 homolog 2 |
Gene namesi | Name:VPS28-2 Ordered Locus Names:At4g05000 ORF Names:T32N4.6 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G05000 |
TAIRi | locus:2138426, AT4G05000 |
Subcellular locationi
Endosome
- Endosome By similarity
Cytosol
- cytosol Source: TAIR
Endosome
- ESCRT I complex Source: TAIR
Keywords - Cellular componenti
EndosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120955 | 1 – 210 | Vacuolar protein sorting-associated protein 28 homolog 2Add BLAST | 210 |
Proteomic databases
PaxDbi | Q9S9T7 |
PRIDEi | Q9S9T7 |
ProteomicsDBi | 242732 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9S9T7, baseline and differential |
Genevisiblei | Q9S9T7, AT |
Interactioni
Subunit structurei
Component of the endosomal sorting required for transport complex I (ESCRT-I), composed of ELC, VPS28 and VPS37.
Interacts with ELC.
1 PublicationBinary interactionsi
Q9S9T7
With | #Exp. | IntAct |
---|---|---|
VPS37-1 [Q9SCP9] | 3 | EBI-3865335,EBI-3865264 |
Protein-protein interaction databases
BioGRIDi | 11153, 7 interactors |
IntActi | Q9S9T7, 3 interactors |
STRINGi | 3702.AT4G05000.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 99 | VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST | 99 | |
Domaini | 109 – 205 | VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST | 97 |
Sequence similaritiesi
Belongs to the VPS28 family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG3284, Eukaryota |
HOGENOMi | CLU_076417_1_0_1 |
InParanoidi | Q9S9T7 |
OMAi | GPETGKW |
OrthoDBi | 1281819at2759 |
PhylomeDBi | Q9S9T7 |
Family and domain databases
Gene3Di | 1.20.120.1130, 1 hit 1.20.1440.200, 1 hit |
InterProi | View protein in InterPro IPR037202, ESCRT_assembly_dom IPR007143, Vps28 IPR017899, VPS28_C IPR037206, VPS28_C_sf IPR017898, VPS28_N IPR038358, VPS28_N_sf |
PANTHERi | PTHR12937, PTHR12937, 1 hit |
Pfami | View protein in Pfam PF03997, VPS28, 1 hit |
PIRSFi | PIRSF017535, VPS28, 1 hit |
SUPFAMi | SSF140111, SSF140111, 1 hit SSF140427, SSF140427, 1 hit |
PROSITEi | View protein in PROSITE PS51310, VPS28_C, 1 hit PS51313, VPS28_N, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9S9T7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMEVKLWNDK REREMYENFA ELFAIIKATE KLEKAYIRDL INPSEYESEC
60 70 80 90 100
QKLIVHFKTL SATLKDTVPN IERFADTYKM DCPAALYRLV TSGLPATVEH
110 120 130 140 150
RATVAASTSN SASIVAECVQ NFITSMDSLK LNMVAVDQVY PLLSDLSASL
160 170 180 190 200
NKLSILPPDF EGKTKMKEWL SRLSKMGAAD ELTEQQSRQL HFDLESSYNS
210
FMAALPKAGN
Sequence cautioni
The sequence AAD48972 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB81042 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF162444 Genomic DNA Translation: AAD48972.1 Different initiation. AL161502 Genomic DNA Translation: CAB81042.1 Different initiation. CP002687 Genomic DNA Translation: AEE82456.1 CP002687 Genomic DNA Translation: AEE82457.1 CP002687 Genomic DNA Translation: ANM66421.1 BT015071 mRNA Translation: AAT71943.1 BT015727 mRNA Translation: AAU45225.1 AK229295 mRNA Translation: BAF01158.1 |
PIRi | H85062 |
RefSeqi | NP_001328317.1, NM_001340525.1 NP_567281.2, NM_116739.4 NP_974513.1, NM_202784.3 |
Genome annotation databases
EnsemblPlantsi | AT4G05000.1; AT4G05000.1; AT4G05000 AT4G05000.2; AT4G05000.2; AT4G05000 AT4G05000.3; AT4G05000.3; AT4G05000 |
GeneIDi | 825842 |
Gramenei | AT4G05000.1; AT4G05000.1; AT4G05000 AT4G05000.2; AT4G05000.2; AT4G05000 AT4G05000.3; AT4G05000.3; AT4G05000 |
KEGGi | ath:AT4G05000 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF162444 Genomic DNA Translation: AAD48972.1 Different initiation. AL161502 Genomic DNA Translation: CAB81042.1 Different initiation. CP002687 Genomic DNA Translation: AEE82456.1 CP002687 Genomic DNA Translation: AEE82457.1 CP002687 Genomic DNA Translation: ANM66421.1 BT015071 mRNA Translation: AAT71943.1 BT015727 mRNA Translation: AAU45225.1 AK229295 mRNA Translation: BAF01158.1 |
PIRi | H85062 |
RefSeqi | NP_001328317.1, NM_001340525.1 NP_567281.2, NM_116739.4 NP_974513.1, NM_202784.3 |
3D structure databases
SMRi | Q9S9T7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 11153, 7 interactors |
IntActi | Q9S9T7, 3 interactors |
STRINGi | 3702.AT4G05000.1 |
Protein family/group databases
TCDBi | 3.A.31.1.2, the endosomal sorting complexes required for transport iii (escrt-iii) family |
Proteomic databases
PaxDbi | Q9S9T7 |
PRIDEi | Q9S9T7 |
ProteomicsDBi | 242732 |
Protocols and materials databases
DNASUi | 825842 |
Genome annotation databases
EnsemblPlantsi | AT4G05000.1; AT4G05000.1; AT4G05000 AT4G05000.2; AT4G05000.2; AT4G05000 AT4G05000.3; AT4G05000.3; AT4G05000 |
GeneIDi | 825842 |
Gramenei | AT4G05000.1; AT4G05000.1; AT4G05000 AT4G05000.2; AT4G05000.2; AT4G05000 AT4G05000.3; AT4G05000.3; AT4G05000 |
KEGGi | ath:AT4G05000 |
Organism-specific databases
Araporti | AT4G05000 |
TAIRi | locus:2138426, AT4G05000 |
Phylogenomic databases
eggNOGi | KOG3284, Eukaryota |
HOGENOMi | CLU_076417_1_0_1 |
InParanoidi | Q9S9T7 |
OMAi | GPETGKW |
OrthoDBi | 1281819at2759 |
PhylomeDBi | Q9S9T7 |
Miscellaneous databases
PROi | PR:Q9S9T7 |
Gene expression databases
ExpressionAtlasi | Q9S9T7, baseline and differential |
Genevisiblei | Q9S9T7, AT |
Family and domain databases
Gene3Di | 1.20.120.1130, 1 hit 1.20.1440.200, 1 hit |
InterProi | View protein in InterPro IPR037202, ESCRT_assembly_dom IPR007143, Vps28 IPR017899, VPS28_C IPR037206, VPS28_C_sf IPR017898, VPS28_N IPR038358, VPS28_N_sf |
PANTHERi | PTHR12937, PTHR12937, 1 hit |
Pfami | View protein in Pfam PF03997, VPS28, 1 hit |
PIRSFi | PIRSF017535, VPS28, 1 hit |
SUPFAMi | SSF140111, SSF140111, 1 hit SSF140427, SSF140427, 1 hit |
PROSITEi | View protein in PROSITE PS51310, VPS28_C, 1 hit PS51313, VPS28_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VP282_ARATH | |
Accessioni | Q9S9T7Primary (citable) accession number: Q9S9T7 Secondary accession number(s): Q6DBF1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 29, 2007 | |
Last modified: | December 2, 2020 | |
This is version 120 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families