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UniProtKB - Q9S7Z3 (PCS1_ARATH)
Protein
Glutathione gamma-glutamylcysteinyltransferase 1
Gene
PCS1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants. Also involved in glutathione-conjugates degradation.
5 PublicationsMiscellaneous
Homoglutathione (hGSH) is a good acceptor, but a poor donor, of gamma-glutamylcysteine units.
Catalytic activityi
- EC:2.3.2.15PROSITE-ProRule annotation
Activity regulationi
Requires cadmium for activity. Also activated in vitro or in heterologous system by Ag+, Hg+, Zn2+, Cu2+, Fe2+ or Fe3+ ions, but not by Co2+ or Ni2+ ions.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 56 | 1 | ||
Active sitei | 162 | 1 | ||
Active sitei | 180 | 1 |
GO - Molecular functioni
- ABC-type phytochelatin transporter activity Source: TAIR
- ATPase-coupled arsenite transmembrane transporter activity Source: TAIR
- cadmium ion binding Source: TAIR
- copper ion binding Source: TAIR
- glutathione gamma-glutamylcysteinyltransferase activity Source: TAIR
GO - Biological processi
- arsenite transport Source: TAIR
- cellular detoxification of cadmium ion Source: GO_Central
- defense response by callose deposition in cell wall Source: TAIR
- defense response to bacterium Source: TAIR
- detoxification of copper ion Source: GO_Central
- indole glucosinolate catabolic process Source: TAIR
- phytochelatin biosynthetic process Source: TAIR
- response to arsenic-containing substance Source: TAIR
- response to cadmium ion Source: TAIR
Keywordsi
Molecular function | Acyltransferase, Transferase |
Ligand | Cadmium, Copper, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | ARA:AT5G44070-MONOMER |
BRENDAi | 2.3.2.15, 399 |
SABIO-RKi | Q9S7Z3 |
Protein family/group databases
MEROPSi | C83.002 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione gamma-glutamylcysteinyltransferase 1 (EC:2.3.2.15)Alternative name(s): Cadmium tolerance protein Phytochelatin synthase 1 Short name: AtPCS1 |
Gene namesi | Name:PCS1 Synonyms:ARA8, CAD1 Ordered Locus Names:At5g44070 ORF Names:MRH10.11, MRH10_18 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G44070 |
TAIRi | locus:2172497, AT5G44070 |
Subcellular locationi
Pathology & Biotechi
Disruption phenotypei
Plants are highly sensitive to Cd (20- to 40-fold) and AsO43- ions, sensitive (eightfold) to Ag ions, slightly sensitive (twofold) to Cu and Hg ions, and insensitive to Zn, SeO32- and Ni ions.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 | Y → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 21 | S → A: No effect. 2 Publications | 1 | |
Mutagenesisi | 56 | C → S or A: Loss of function. 2 Publications | 1 | |
Mutagenesisi | 59 | A → V in cad1-4; loss of function. 1 Publication | 1 | |
Mutagenesisi | 71 | D → A: Decreased activity. 1 Publication | 1 | |
Mutagenesisi | 84 | D → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 89 | D → A: Slightly decreased activity. 1 Publication | 1 | |
Mutagenesisi | 90 | C → S or A: Decreased activity. 2 Publications | 1 | |
Mutagenesisi | 91 | C → S or A: No effect. 3 Publications | 1 | |
Mutagenesisi | 91 | C → Y in cad1-1; loss of function. 3 Publications | 1 | |
Mutagenesisi | 109 | C → S or A: No effect. 2 Publications | 1 | |
Mutagenesisi | 113 | C → S or A: No effect. 2 Publications | 1 | |
Mutagenesisi | 158 | T → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 162 | H → A: Loss of function. 1 Publication | 1 | |
Mutagenesisi | 164 | S → A: No effect. 2 Publications | 1 | |
Mutagenesisi | 174 | D → A: Decreased activity. 1 Publication | 1 | |
Mutagenesisi | 180 | D → A: Loss of function. 1 Publication | 1 | |
Mutagenesisi | 186 | Y → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 189 | H → A: Decreased activity. 1 Publication | 1 | |
Mutagenesisi | 190 | W → C in cad1-3; loss of function. 1 Publication | 1 | |
Mutagenesisi | 204 | D → A: Decreased activity. 1 Publication | 1 | |
Mutagenesisi | 220 | H → A: Decreased activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000287210 | 1 – 485 | Glutathione gamma-glutamylcysteinyltransferase 1Add BLAST | 485 |
Proteomic databases
PaxDbi | Q9S7Z3 |
PRIDEi | Q9S7Z3 |
ProteomicsDBi | 236374 |
PTM databases
iPTMneti | Q9S7Z3 |
Expressioni
Tissue specificityi
Expressed in roots and shoots.2 Publications
Developmental stagei
Expressed constitutively.1 Publication
Inductioni
Induced by copper, and by cadmium during the early stages of plant development. Gradual decrease of cadmium induction as plants continue to grow, and by 15 day after germination, total loss of induction.2 Publications
Gene expression databases
ExpressionAtlasi | Q9S7Z3, baseline and differential |
Genevisiblei | Q9S7Z3, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 19680, 3 interactors |
IntActi | Q9S7Z3, 1 interactor |
STRINGi | 3702.AT5G44070.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 221 | Peptidase C83PROSITE-ProRule annotationAdd BLAST | 221 |
Domaini
The intermediate part (284-372) is important for enzyme stabilization.
The C-terminal part (373-485) is required to determine enzyme responsiveness to a broad range of heavy metals.
Sequence similaritiesi
Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0632, Eukaryota |
HOGENOMi | CLU_046059_0_0_1 |
InParanoidi | Q9S7Z3 |
OMAi | CKHESWA |
OrthoDBi | 1279063at2759 |
PhylomeDBi | Q9S7Z3 |
Family and domain databases
Gene3Di | 3.90.70.30, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR040409, PCS-like IPR007719, PCS_N IPR038156, PCS_N_sf IPR015407, Phytochelatin_synthase_C |
PANTHERi | PTHR33447, PTHR33447, 1 hit |
Pfami | View protein in Pfam PF05023, Phytochelatin, 1 hit PF09328, Phytochelatin_C, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51443, PCS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9S7Z3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAMASLYRRS LPSPPAIDFS SAEGKLIFNE ALQKGTMEGF FRLISYFQTQ
60 70 80 90 100
SEPAYCGLAS LSVVLNALSI DPGRKWKGPW RWFDESMLDC CEPLEVVKEK
110 120 130 140 150
GISFGKVVCL AHCSGAKVEA FRTSQSTIDD FRKFVVKCTS SENCHMISTY
160 170 180 190 200
HRGVFKQTGT GHFSPIGGYN AERDMALILD VARFKYPPHW VPLKLLWEAM
210 220 230 240 250
DSIDQSTGKR RGFMLISRPH REPGLLYTLS CKDESWIEIA KYLKEDVPRL
260 270 280 290 300
VSSQHVDSVE KIISVVFKSL PSNFNQFIRW VAEIRITEDS NQNLSAEEKS
310 320 330 340 350
RLKLKQLVLK EVHETELFKH INKFLSTVGY EDSLTYAAAK ACCQGAEILS
360 370 380 390 400
GSPSKEFCCR ETCVKCIKGP DDSEGTVVTG VVVRDGNEQK VDLLVPSTQT
410 420 430 440 450
ECECGPEATY PAGNDVFTAL LLALPPQTWS GIKDQALMHE MKQLISMASL
460 470 480
PTLLQEEVLH LRRQLQLLKR CQENKEEDDL AAPAY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BHA6 | A0A1P8BHA6_ARATH | Glutathione gamma-glutamylcysteinyl... | CAD1 ARA8, ATPCS1, CADMIUM SENSITIVE 1, MRH10.18, MRH10_18 | 449 | Annotation score: |
Sequence cautioni
The sequence AAD29446 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 153 | G → S in AAD16046 (PubMed:10359847).Curated | 1 | |
Sequence conflicti | 160 | T → N in AAD16046 (PubMed:10359847).Curated | 1 | |
Sequence conflicti | 298 | E → A in AAL66747 (PubMed:12514032).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF135155 mRNA Translation: AAD41794.1 AF093753 mRNA Translation: AAD50593.1 AF085230 mRNA Translation: AAD16046.1 AF085231 Genomic DNA Translation: AAD29446.1 Sequence problems. AF162689 mRNA Translation: AAF42805.1 AF461180 mRNA Translation: AAL66747.1 AB006703 Genomic DNA Translation: BAB09067.1 CP002688 Genomic DNA Translation: AED95055.1 AY079384 mRNA Translation: AAL85115.1 AY048257 mRNA Translation: AAK82519.1 AY039951 mRNA Translation: AAK64055.1 |
RefSeqi | NP_199220.1, NM_123774.4 |
Genome annotation databases
EnsemblPlantsi | AT5G44070.1; AT5G44070.1; AT5G44070 |
GeneIDi | 834430 |
Gramenei | AT5G44070.1; AT5G44070.1; AT5G44070 |
KEGGi | ath:AT5G44070 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF135155 mRNA Translation: AAD41794.1 AF093753 mRNA Translation: AAD50593.1 AF085230 mRNA Translation: AAD16046.1 AF085231 Genomic DNA Translation: AAD29446.1 Sequence problems. AF162689 mRNA Translation: AAF42805.1 AF461180 mRNA Translation: AAL66747.1 AB006703 Genomic DNA Translation: BAB09067.1 CP002688 Genomic DNA Translation: AED95055.1 AY079384 mRNA Translation: AAL85115.1 AY048257 mRNA Translation: AAK82519.1 AY039951 mRNA Translation: AAK64055.1 |
RefSeqi | NP_199220.1, NM_123774.4 |
3D structure databases
AlphaFoldDBi | Q9S7Z3 |
SMRi | Q9S7Z3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 19680, 3 interactors |
IntActi | Q9S7Z3, 1 interactor |
STRINGi | 3702.AT5G44070.1 |
Protein family/group databases
MEROPSi | C83.002 |
PTM databases
iPTMneti | Q9S7Z3 |
Proteomic databases
PaxDbi | Q9S7Z3 |
PRIDEi | Q9S7Z3 |
ProteomicsDBi | 236374 |
Genome annotation databases
EnsemblPlantsi | AT5G44070.1; AT5G44070.1; AT5G44070 |
GeneIDi | 834430 |
Gramenei | AT5G44070.1; AT5G44070.1; AT5G44070 |
KEGGi | ath:AT5G44070 |
Organism-specific databases
Araporti | AT5G44070 |
TAIRi | locus:2172497, AT5G44070 |
Phylogenomic databases
eggNOGi | KOG0632, Eukaryota |
HOGENOMi | CLU_046059_0_0_1 |
InParanoidi | Q9S7Z3 |
OMAi | CKHESWA |
OrthoDBi | 1279063at2759 |
PhylomeDBi | Q9S7Z3 |
Enzyme and pathway databases
BioCyci | ARA:AT5G44070-MONOMER |
BRENDAi | 2.3.2.15, 399 |
SABIO-RKi | Q9S7Z3 |
Miscellaneous databases
PROi | PR:Q9S7Z3 |
Gene expression databases
ExpressionAtlasi | Q9S7Z3, baseline and differential |
Genevisiblei | Q9S7Z3, AT |
Family and domain databases
Gene3Di | 3.90.70.30, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR040409, PCS-like IPR007719, PCS_N IPR038156, PCS_N_sf IPR015407, Phytochelatin_synthase_C |
PANTHERi | PTHR33447, PTHR33447, 1 hit |
Pfami | View protein in Pfam PF05023, Phytochelatin, 1 hit PF09328, Phytochelatin_C, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51443, PCS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PCS1_ARATH | |
Accessioni | Q9S7Z3Primary (citable) accession number: Q9S7Z3 Secondary accession number(s): Q8W122 Q9ZPM2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families