UniProtKB - Q9S7Q7 (FLC_ARATH)
Protein
MADS-box protein FLOWERING LOCUS C
Gene
FLC
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Putative transcription factor that seems to play a central role in the regulation of flowering time in the late-flowering phenotype by interacting with 'FRIGIDA', the autonomous and the vernalization flowering pathways. Inhibits flowering by repressing 'SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1'.3 Publications
Miscellaneous
Prevention of early flowering by expression of FLC requires methylation of 'Lys-36' of histone H3. Repression of FLC is dependent on histone H3 'Lys-4' methylation which is partly controlled by the lysine-specific demthylase 1 (LSD1) homologs, LDL1, LDL2 and FLD. May also be regulated by the level of histone acetylation.
GO - Molecular functioni
- DNA-binding transcription factor activity Source: TAIR
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: TAIR
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- floral meristem determinacy Source: GO_Central
- negative regulation of flower development Source: TAIR
- positive regulation of transcription by RNA polymerase II Source: InterPro
- regulation of circadian rhythm Source: TAIR
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to temperature stimulus Source: TAIR
- vernalization response Source: TAIR
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Flowering, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: MADS-box protein FLOWERING LOCUS C1 PublicationAlternative name(s): MADS-box protein FLOWERING LOCUS F1 Publication |
Gene namesi | Name:FLC1 Publication Synonyms:FLF1 Publication Ordered Locus Names:At5g10140Imported ORF Names:T31P16.130Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G10140 |
TAIRi | locus:2184118, AT5G10140 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- protein-containing complex Source: TAIR
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199481 | 1 – 196 | MADS-box protein FLOWERING LOCUS CAdd BLAST | 196 |
Proteomic databases
PaxDbi | Q9S7Q7 |
PRIDEi | Q9S7Q7 |
ProteomicsDBi | 230426 [Q9S7Q7-1] |
Expressioni
Tissue specificityi
High expression in the vegetative apex and in root tissue and lower expression in leaves and stems. Not detected in young tissues of the inflorescence. Before fertilization, expressed in ovules, but not in pollen or stamens, of non-vernalized plants. After vernalization, not detected in ovules.1 Publication
Developmental stagei
Found in shoots of non-flowering plants grown under long-day conditions at days 4 to 15, and in shoots of plants grown under short-day conditions at days 4 to 11 after germination (PubMed:19121105). Expressed in embryos from the early globular stage (PubMed:19121105). FLC is not imprinted and both parental alleles contribute equally to expression in embryos (PubMed:19121105). Expression is repressed during gametogenesis, and is then reactivated after fertilization in embryos (PubMed:19121105). In seedlings, observed in the vasculature of the cotyledons, hypocotyls, and the first pair of leaves (PubMed:18375656). Just prior to flowering, a strong reduction in expression levels occurs in the vasculature (PubMed:18375656).2 Publications
Inductioni
Epigenetically down-regulated by vernalization. Vernalization repression is initiated by VIN3. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2. Up-regulated by HUA2. Down-regulated by VOZ1 and/or VOZ2. Down-regulated by RBG7.5 Publications
Gene expression databases
ExpressionAtlasi | Q9S7Q7, baseline and differential |
Genevisiblei | Q9S7Q7, AT |
Interactioni
Binary interactionsi
Q9S7Q7
With | #Exp. | IntAct |
---|---|---|
SVP [Q9FVC1] | 4 | EBI-2127872,EBI-592058 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 16156, 13 interactors |
DIPi | DIP-51640N |
IntActi | Q9S7Q7, 7 interactors |
STRINGi | 3702.AT5G10140.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 61 | MADS-boxPROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 80 – 170 | K-boxPROSITE-ProRule annotationAdd BLAST | 91 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 8 – 15 | Nuclear localization signalPROSITE-ProRule annotation | 8 |
Phylogenomic databases
eggNOGi | KOG0014, Eukaryota |
HOGENOMi | CLU_053053_0_4_1 |
InParanoidi | Q9S7Q7 |
OMAi | ANELRIW |
PhylomeDBi | Q9S7Q7 |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR033896, MADS_MEF2-like IPR002487, TF_Kbox IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF01486, K-box, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS51297, K_BOX, 1 hit PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9S7Q7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGRKKLEIKR IENKSSRQVT FSKRRNGLIE KARQLSVLCD ASVALLVVSA
60 70 80 90 100
SGKLYSFSSG DNLVKILDRY GKQHADDLKA LDHQSKALNY GSHYELLELV
110 120 130 140 150
DSKLVGSNVK NVSIDALVQL EEHLETALSV TRAKKTELML KLVENLKEKE
160 170 180 190
KMLKEENQVL ASQMENNHHV GAEAEMEMSP AGQISDNLPV TLPLLN
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ58T15 | Q58T15_ARATH | Flowering locus C protein | FLC AGAMOUS-like 25, AGL25, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F | 167 | Annotation score: | ||
A5JM49 | A5JM49_ARATH | Flowering locus C beta splice varia... | FLC AGAMOUS-like 25, AGL25, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F | 186 | Annotation score: | ||
Q58T14 | Q58T14_ARATH | Flowering locus C protein | FLC AGAMOUS-like 25, AGL25, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F | 182 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF116527 mRNA Translation: AAD21248.1 AF116528 Genomic DNA Translation: AAD21249.1 AL356332 Genomic DNA Translation: CAB92055.1 CP002688 Genomic DNA Translation: AED91498.1 |
PIRi | T50018 |
RefSeqi | NP_196576.1, NM_121052.3 [Q9S7Q7-1] |
Genome annotation databases
EnsemblPlantsi | AT5G10140.1; AT5G10140.1; AT5G10140 [Q9S7Q7-1] |
GeneIDi | 830878 |
Gramenei | AT5G10140.1; AT5G10140.1; AT5G10140 [Q9S7Q7-1] |
KEGGi | ath:AT5G10140 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF116527 mRNA Translation: AAD21248.1 AF116528 Genomic DNA Translation: AAD21249.1 AL356332 Genomic DNA Translation: CAB92055.1 CP002688 Genomic DNA Translation: AED91498.1 |
PIRi | T50018 |
RefSeqi | NP_196576.1, NM_121052.3 [Q9S7Q7-1] |
3D structure databases
SMRi | Q9S7Q7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 16156, 13 interactors |
DIPi | DIP-51640N |
IntActi | Q9S7Q7, 7 interactors |
STRINGi | 3702.AT5G10140.1 |
Proteomic databases
PaxDbi | Q9S7Q7 |
PRIDEi | Q9S7Q7 |
ProteomicsDBi | 230426 [Q9S7Q7-1] |
Genome annotation databases
EnsemblPlantsi | AT5G10140.1; AT5G10140.1; AT5G10140 [Q9S7Q7-1] |
GeneIDi | 830878 |
Gramenei | AT5G10140.1; AT5G10140.1; AT5G10140 [Q9S7Q7-1] |
KEGGi | ath:AT5G10140 |
Organism-specific databases
Araporti | AT5G10140 |
TAIRi | locus:2184118, AT5G10140 |
Phylogenomic databases
eggNOGi | KOG0014, Eukaryota |
HOGENOMi | CLU_053053_0_4_1 |
InParanoidi | Q9S7Q7 |
OMAi | ANELRIW |
PhylomeDBi | Q9S7Q7 |
Miscellaneous databases
PROi | PR:Q9S7Q7 |
Gene expression databases
ExpressionAtlasi | Q9S7Q7, baseline and differential |
Genevisiblei | Q9S7Q7, AT |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR033896, MADS_MEF2-like IPR002487, TF_Kbox IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF01486, K-box, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS51297, K_BOX, 1 hit PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FLC_ARATH | |
Accessioni | Q9S7Q7Primary (citable) accession number: Q9S7Q7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 5, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names