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Protein

LRR receptor-like serine/threonine-protein kinase RPK2

Gene

RPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Key regulator of anther development (e.g. lignification pattern), including tapetum degradation during pollen maturation (e.g. germination capacity). Together with RPK1, required for pattern formation along the radial axis (e.g. the apical embryonic domain cell types that generate cotyledon primordia), and the apical-basal axis (e.g. differentiation of the basal pole during early embryogenesis).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei902ATPPROSITE-ProRule annotation1
Active sitei996Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi880 – 888ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • anther development Source: UniProtKB
  • embryonic meristem development Source: UniProtKB
  • lignin metabolic process Source: TAIR
  • longitudinal axis specification Source: UniProtKB
  • meristem maintenance Source: TAIR
  • pollen germination Source: UniProtKB
  • pollen maturation Source: TAIR
  • protein homooligomerization Source: TAIR
  • radial axis specification Source: UniProtKB
  • response to cold Source: UniProtKB
  • response to water deprivation Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
LRR receptor-like serine/threonine-protein kinase RPK2 (EC:2.7.11.1)
Alternative name(s):
Protein TOADSTOOL 2
Receptor-like protein kinase 2
Gene namesi
Name:RPK2
Synonyms:TOAD2
Ordered Locus Names:At3g02130
ORF Names:F14P3.22, F1C9.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G02130
TAIRilocus:2076512 AT3G02130

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini36 – 811ExtracellularSequence analysisAdd BLAST776
Transmembranei812 – 832HelicalSequence analysisAdd BLAST21
Topological domaini833 – 1151CytoplasmicSequence analysisAdd BLAST319

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired central domain protoderm patterning defects, and defective cotyledon primordia cell types. Enhanced shoot growth, and male sterility due to defects in anther dehiscence and pollen maturation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000038752336 – 1151LRR receptor-like serine/threonine-protein kinase RPK2Add BLAST1116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi214N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi229N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi332N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi481N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi540N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi591N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi640N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi670N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi708N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi718N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi734N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi739N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei863PhosphothreonineBy similarity1
Modified residuei871PhosphothreonineBy similarity1
Modified residuei947PhosphotyrosineBy similarity1
Modified residuei983PhosphotyrosineBy similarity1
Modified residuei1038PhosphotyrosineBy similarity1
Modified residuei1046PhosphothreonineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9S7I6
PRIDEiQ9S7I6

Expressioni

Tissue specificityi

Expressed in root tips, flowers, leaf meristems, and the bottom parts of the surrounding young leaves and trichomes.1 Publication

Developmental stagei

First detected in the central domain protodermal cells when cotyledon primordia become recognizable, at the early globular stage. Later observed throughout the central domain and basal domain of the embryo proper. After bolting, localized in axillary buds and premature anthers. Abundant in the tapetum of wild-type anthers during microspore maturation.3 Publications

Inductioni

Slightly by dehydration and low temperature.1 Publication

Gene expression databases

ExpressionAtlasiQ9S7I6 baseline and differential
GenevisibleiQ9S7I6 AT

Interactioni

Protein-protein interaction databases

BioGridi6573, 40 interactors
IntActiQ9S7I6, 23 interactors
STRINGi3702.AT3G02130.1

Structurei

3D structure databases

ProteinModelPortaliQ9S7I6
SMRiQ9S7I6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati85 – 108LRR 1Add BLAST24
Repeati143 – 167LRR 2Add BLAST25
Repeati168 – 190LRR 3Add BLAST23
Repeati192 – 215LRR 4Add BLAST24
Repeati216 – 241LRR 5Add BLAST26
Repeati261 – 285LRR 6Add BLAST25
Repeati287 – 308LRR 7Add BLAST22
Repeati309 – 333LRR 8Add BLAST25
Repeati341 – 368LRR 9Add BLAST28
Repeati387 – 410LRR 10Add BLAST24
Repeati411 – 435LRR 11Add BLAST25
Repeati436 – 459LRR 12Add BLAST24
Repeati461 – 481LRR 13Add BLAST21
Repeati542 – 565LRR 14Add BLAST24
Repeati567 – 581LRR 15Add BLAST15
Repeati585 – 608LRR 16Add BLAST24
Repeati610 – 633LRR 17Add BLAST24
Repeati634 – 657LRR 18Add BLAST24
Repeati658 – 681LRR 19Add BLAST24
Repeati682 – 706LRR 20Add BLAST25
Repeati707 – 729LRR 21Add BLAST23
Repeati731 – 752LRR 22Add BLAST22
Domaini874 – 1146Protein kinasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHEJ Eukaryota
COG0515 LUCA
COG4886 LUCA
HOGENOMiHOG000116551
InParanoidiQ9S7I6
OMAiPVGLSKC
OrthoDBiEOG093601XL
PhylomeDBiQ9S7I6

Family and domain databases

Gene3Di3.80.10.10, 3 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR013210 LRR_N_plant-typ
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00560 LRR_1, 1 hit
PF13855 LRR_8, 2 hits
PF08263 LRRNT_2, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 9 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9S7I6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLPSSVIK WRFFRRQMPS DVVFSLCLLC FASCLAGKIT VLADSDKSVL
60 70 80 90 100
LRFKKTVSDP GSILASWVEE SEDYCSWFGV SCDSSSRVMA LNISGSGSSE
110 120 130 140 150
ISRNRFTCGD IGKFPLYGFG VRRDCTGNHG ALAGNLPSVI MSLTGLRVLS
160 170 180 190 200
LPFNSFSGEI PVGIWGMEKL EVLDLEGNLM TGSLPDQFTG LRNLRVMNLG
210 220 230 240 250
FNRVSGEIPN SLQNLTKLEI LNLGGNKLNG TVPGFVGRFR VLHLPLNWLQ
260 270 280 290 300
GSLPKDIGDS CGKLEHLDLS GNFLTGRIPE SLGKCAGLRS LLLYMNTLEE
310 320 330 340 350
TIPLEFGSLQ KLEVLDVSRN TLSGPLPVEL GNCSSLSVLV LSNLYNVYED
360 370 380 390 400
INSVRGEADL PPGADLTSMT EDFNFYQGGI PEEITRLPKL KILWVPRATL
410 420 430 440 450
EGRFPGDWGS CQNLEMVNLG QNFFKGEIPV GLSKCKNLRL LDLSSNRLTG
460 470 480 490 500
ELLKEISVPC MSVFDVGGNS LSGVIPDFLN NTTSHCPPVV YFDRFSIESY
510 520 530 540 550
SDPSSVYLSF FTEKAQVGTS LIDLGSDGGP AVFHNFADNN FTGTLKSIPL
560 570 580 590 600
AQERLGKRVS YIFSAGGNRL YGQFPGNLFD NCDELKAVYV NVSFNKLSGR
610 620 630 640 650
IPQGLNNMCT SLKILDASVN QIFGPIPTSL GDLASLVALN LSWNQLQGQI
660 670 680 690 700
PGSLGKKMAA LTYLSIANNN LTGQIPQSFG QLHSLDVLDL SSNHLSGGIP
710 720 730 740 750
HDFVNLKNLT VLLLNNNNLS GPIPSGFATF AVFNVSSNNL SGPVPSTNGL
760 770 780 790 800
TKCSTVSGNP YLRPCHVFSL TTPSSDSRDS TGDSITQDYA SSPVENAPSQ
810 820 830 840 850
SPGKGGFNSL EIASIASASA IVSVLIALVI LFFYTRKWHP KSKIMATTKR
860 870 880 890 900
EVTMFMDIGV PITFDNVVRA TGNFNASNLI GNGGFGATYK AEISQDVVVA
910 920 930 940 950
IKRLSIGRFQ GVQQFHAEIK TLGRLRHPNL VTLIGYHASE TEMFLVYNYL
960 970 980 990 1000
PGGNLEKFIQ ERSTRDWRVL HKIALDIARA LAYLHDQCVP RVLHRDVKPS
1010 1020 1030 1040 1050
NILLDDDCNA YLSDFGLARL LGTSETHATT GVAGTFGYVA PEYAMTCRVS
1060 1070 1080 1090 1100
DKADVYSYGV VLLELLSDKK ALDPSFVSYG NGFNIVQWAC MLLRQGRAKE
1110 1120 1130 1140 1150
FFTAGLWDAG PHDDLVEVLH LAVVCTVDSL STRPTMKQVV RRLKQLQPPS

C
Length:1,151
Mass (Da):125,230
Last modified:May 1, 2000 - v1
Checksum:i86DECE2E18F11326
GO

Sequence cautioni

The sequence BAC42107 differs from that shown. Reason: Frameshift at position 1101.Curated
The sequence BAC42107 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009755 Genomic DNA Translation: AAF02124.1
AC011664 Genomic DNA Translation: AAF14849.1
CP002686 Genomic DNA Translation: AEE73767.1
FJ708715 mRNA Translation: ACN59310.1
AK117442 mRNA Translation: BAC42107.1 Sequence problems.
RefSeqiNP_186862.3, NM_111080.4
UniGeneiAt.41177

Genome annotation databases

EnsemblPlantsiAT3G02130.1; AT3G02130.1; AT3G02130
GeneIDi821240
GrameneiAT3G02130.1; AT3G02130.1; AT3G02130
KEGGiath:AT3G02130

Similar proteinsi

Entry informationi

Entry nameiRPK2_ARATH
AccessioniPrimary (citable) accession number: Q9S7I6
Secondary accession number(s): C0LGM5, Q8GYR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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