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Protein

Single-stranded DNA-binding protein

Gene

ssb

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism (By similarity). Essential for ionizing radiation resistance. Stimulates the 5'-3' DNA helicase activity of RecD-like helicase. Stimulates RecA protein-promoted DNA three-strand exchange reactions in vitro with both D.radiodurans and E.coli-derived RecA. Complements an ssb deletion in E.coli, but does not complement a ddrb disruption in D.radiodurans.UniRule annotation4 Publications

GO - Molecular functioni

  • single-stranded DNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA recombination, DNA repair, DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA-binding proteinUniRule annotation
Short name:
SSBUniRule annotation
Gene namesi
Name:ssb
Ordered Locus Names:DR_0099
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Essential, it cannot be knocked out. As SSB levels are depleted growth slows, tolerance to ionizing radiation and UV light decreases rapidly. Cannot be complemented by ddrB.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000961471 – 301Single-stranded DNA-binding proteinAdd BLAST301

Proteomic databases

PRIDEiQ9RY51

Expressioni

Inductioni

Constitutively expressed, highly up-regulated following ionizing radiation for at least 12 hours (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer. Binds ssDNA. Interacts with DdrB.5 Publications

Protein-protein interaction databases

STRINGi243230.DR_0100

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9RY51
SMRiQ9RY51
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9RY51

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 110SSB 1UniRule annotationAdd BLAST106
Domaini129 – 232SSB 2UniRule annotationAdd BLAST104

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi296 – 301Important for interaction with partner proteinsUniRule annotation6

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107YH6 Bacteria
COG0629 LUCA
InParanoidiQ9RY51
KOiK03111
OMAiARGMNHV
OrthoDBiPOG091H00DP

Family and domain databases

CDDicd04496 SSB_OBF, 2 hits
HAMAPiMF_00984 SSB, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR000424 Primosome_PriB/ssb
IPR011344 ssDNA-bd
PANTHERiPTHR10302 PTHR10302, 2 hits
PTHR10302:SF0 PTHR10302:SF0, 2 hits
PfamiView protein in Pfam
PF00436 SSB, 2 hits
SUPFAMiSSF50249 SSF50249, 2 hits
TIGRFAMsiTIGR00621 ssb, 2 hits
PROSITEiView protein in PROSITE
PS50935 SSB, 2 hits

Sequencei

Sequence statusi: Complete.

Q9RY51-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARGMNHVYL IGALARDPEL RYTGNGMAVF EATVAGEDRV IGNDGRERNL
60 70 80 90 100
PWYHRVSILG KPAEWQAERN LKGGDAVVVE GTLEYRQWEA PEGGKRSAVN
110 120 130 140 150
VKALRMEQLG TQPELIQDAG GGVRMSGAMN EVLVLGNVTR DPEIRYTPAG
160 170 180 190 200
DAVLSLSIAV NENYQDRQGQ RQEKVHYIDA TLWRDLAENM KELRKGDPVM
210 220 230 240 250
IMGRLVNEGW TDKDGNKRNS TRVEATRVEA LARGAGNANS GYAAATPAAP
260 270 280 290 300
RTQTASSAAR PTSGGYQSQP SRAANTGSRS GGLDIDQGLD DFPPEEDDLP

F
Length:301
Mass (Da):32,722
Last modified:October 3, 2003 - v2
Checksum:i21B2090C970E1B0E
GO

Sequence cautioni

The sequence AAF09692 differs from that shown. Reason: Frameshift at positions 93 and 169.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY293617 Genomic DNA Translation: AAQ18705.1
AJ564860 Genomic DNA Translation: CAD92322.1
AE000513 Genomic DNA Translation: AAF09692.1 Frameshift.
PIRiA75559
RefSeqiNP_293825.1, NC_001263.1

Genome annotation databases

EnsemblBacteriaiAAF09692; AAF09692; DR_0099
GeneIDi1799251
KEGGidra:DR_0099
PATRICifig|243230.17.peg.263

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY293617 Genomic DNA Translation: AAQ18705.1
AJ564860 Genomic DNA Translation: CAD92322.1
AE000513 Genomic DNA Translation: AAF09692.1 Frameshift.
PIRiA75559
RefSeqiNP_293825.1, NC_001263.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SE8X-ray1.80A1-301[»]
3UDGX-ray2.40A/B/C1-301[»]
3UFMX-ray1.95B290-301[»]
ProteinModelPortaliQ9RY51
SMRiQ9RY51
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_0100

Proteomic databases

PRIDEiQ9RY51

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF09692; AAF09692; DR_0099
GeneIDi1799251
KEGGidra:DR_0099
PATRICifig|243230.17.peg.263

Phylogenomic databases

eggNOGiENOG4107YH6 Bacteria
COG0629 LUCA
InParanoidiQ9RY51
KOiK03111
OMAiARGMNHV
OrthoDBiPOG091H00DP

Miscellaneous databases

EvolutionaryTraceiQ9RY51

Family and domain databases

CDDicd04496 SSB_OBF, 2 hits
HAMAPiMF_00984 SSB, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR000424 Primosome_PriB/ssb
IPR011344 ssDNA-bd
PANTHERiPTHR10302 PTHR10302, 2 hits
PTHR10302:SF0 PTHR10302:SF0, 2 hits
PfamiView protein in Pfam
PF00436 SSB, 2 hits
SUPFAMiSSF50249 SSF50249, 2 hits
TIGRFAMsiTIGR00621 ssb, 2 hits
PROSITEiView protein in PROSITE
PS50935 SSB, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSSB_DEIRA
AccessioniPrimary (citable) accession number: Q9RY51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 7, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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