UniProtKB - Q9RXJ7 (RL22_DEIRA)
50S ribosomal protein L22
rplV
Functioni
This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity).
By similarityThe globular domain of the protein is located by the polypeptide exit tunnel on the outside of the subunit while an extended beta-hairpin forms part of the wall of the tunnel. Forms a pair of 'tweezers' with L32 that hold together two different domains of the 23S rRNA. Interacts with the tunnel-blocking modified macrolide azithromycin. Upon binding of the macrolide troleadomycin to the ribosome, the tip of the beta-hairpin is displaced, which severely restricts the tunnel. This and experiments in E.coli have led to the suggestion that it is part of the gating mechanism involved in translation arrest in the absence of the protein export system.
GO - Molecular functioni
- rRNA binding Source: UniProtKB-UniRule
- structural constituent of ribosome Source: GO_Central
GO - Biological processi
- ribosome assembly Source: GO_Central
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 50S ribosomal protein L22 |
Gene namesi | Name:rplV Ordered Locus Names:DR_0316 |
Organismi | Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) |
Taxonomic identifieri | 243230 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosolic large ribosomal subunit Source: GO_Central
Other locations
- large ribosomal subunit Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000125151 | 1 – 134 | 50S ribosomal protein L22Add BLAST | 134 |
Interactioni
Subunit structurei
Part of the 50S ribosomal subunit. Contacts protein L32.
6 PublicationsProtein-protein interaction databases
IntActi | Q9RXJ7, 1 interactor |
STRINGi | 243230.DR_0316 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9RXJ7 |
SMRi | Q9RXJ7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9RXJ7 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0091, Bacteria |
HOGENOMi | CLU_083987_3_1_0 |
InParanoidi | Q9RXJ7 |
OMAi | KRIQPRA |
OrthoDBi | 1666043at2 |
Family and domain databases
CDDi | cd00336, Ribosomal_L22, 1 hit |
Gene3Di | 3.90.470.10, 1 hit |
HAMAPi | MF_01331_B, Ribosomal_L22_B, 1 hit |
InterProi | View protein in InterPro IPR001063, Ribosomal_L22 IPR018260, Ribosomal_L22/L17_CS IPR036394, Ribosomal_L22/L17_sf IPR005727, Ribosomal_L22_bac/chlpt-type |
PANTHERi | PTHR13501, PTHR13501, 1 hit |
Pfami | View protein in Pfam PF00237, Ribosomal_L22, 1 hit |
SUPFAMi | SSF54843, SSF54843, 1 hit |
TIGRFAMsi | TIGR01044, rplV_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00464, RIBOSOMAL_L22, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTAPEQTFRN KKQRKQQVKL RKPGFAVAKY VRMSPRKVRL VVDVIRGKSV
60 70 80 90 100
QDAEDLLRFI PRSASEPVAK VLNSAKANAL HNDEMLEDRL FVKEAYVDAG
110 120 130
PTLKRLIPRA RGSANIIKKR TSHITIIVAE KGNK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000513 Genomic DNA Translation: AAF09897.1 |
PIRi | D75534 |
RefSeqi | NP_294039.1, NC_001263.1 WP_010886961.1, NZ_CP015081.1 |
Genome annotation databases
EnsemblBacteriai | AAF09897; AAF09897; DR_0316 |
KEGGi | dra:DR_0316 |
PATRICi | fig|243230.17.peg.482 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000513 Genomic DNA Translation: AAF09897.1 |
PIRi | D75534 |
RefSeqi | NP_294039.1, NC_001263.1 WP_010886961.1, NZ_CP015081.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1J5A | X-ray | 3.50 | L | 1-134 | [»] | |
1JZX | X-ray | 3.10 | L | 1-134 | [»] | |
1JZY | X-ray | 3.50 | L | 1-134 | [»] | |
1JZZ | X-ray | 3.80 | L | 1-134 | [»] | |
1K01 | X-ray | 3.50 | L | 1-134 | [»] | |
1NKW | X-ray | 3.10 | Q | 1-134 | [»] | |
1NWX | X-ray | 3.50 | Q | 1-134 | [»] | |
1NWY | X-ray | 3.30 | Q | 1-134 | [»] | |
1OND | X-ray | 3.40 | Q | 1-134 | [»] | |
1SM1 | X-ray | 3.42 | Q | 1-134 | [»] | |
1XBP | X-ray | 3.50 | Q | 1-134 | [»] | |
2ZJP | X-ray | 3.70 | P | 1-134 | [»] | |
2ZJQ | X-ray | 3.30 | P | 1-134 | [»] | |
2ZJR | X-ray | 2.91 | P | 1-134 | [»] | |
3CF5 | X-ray | 3.30 | P | 1-134 | [»] | |
3DLL | X-ray | 3.50 | P | 1-134 | [»] | |
3PIO | X-ray | 3.25 | P | 1-134 | [»] | |
3PIP | X-ray | 3.45 | P | 1-134 | [»] | |
4IO9 | X-ray | 3.20 | P | 1-134 | [»] | |
4IOA | X-ray | 3.20 | P | 1-134 | [»] | |
4IOC | X-ray | 3.60 | P | 1-134 | [»] | |
4U67 | X-ray | 3.65 | P | 1-134 | [»] | |
4V49 | X-ray | 8.70 | Q | 5-134 | [»] | |
4V4A | X-ray | 9.50 | Q | 5-134 | [»] | |
4V4G | X-ray | 11.50 | T | 5-134 | [»] | |
4WFN | X-ray | 3.54 | P | 1-134 | [»] | |
5DM6 | X-ray | 2.90 | P | 8-134 | [»] | |
5DM7 | X-ray | 3.00 | P | 8-134 | [»] | |
5JVG | X-ray | 3.43 | P | 1-134 | [»] | |
5JVH | X-ray | 3.58 | P | 1-134 | [»] | |
7A0R | X-ray | 3.30 | P | 6-133 | [»] | |
7A0S | X-ray | 3.22 | P | 1-134 | [»] | |
7A18 | X-ray | 3.40 | P | 6-134 | [»] | |
AlphaFoldDBi | Q9RXJ7 | |||||
SMRi | Q9RXJ7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | Q9RXJ7, 1 interactor |
STRINGi | 243230.DR_0316 |
Genome annotation databases
EnsemblBacteriai | AAF09897; AAF09897; DR_0316 |
KEGGi | dra:DR_0316 |
PATRICi | fig|243230.17.peg.482 |
Phylogenomic databases
eggNOGi | COG0091, Bacteria |
HOGENOMi | CLU_083987_3_1_0 |
InParanoidi | Q9RXJ7 |
OMAi | KRIQPRA |
OrthoDBi | 1666043at2 |
Miscellaneous databases
EvolutionaryTracei | Q9RXJ7 |
Family and domain databases
CDDi | cd00336, Ribosomal_L22, 1 hit |
Gene3Di | 3.90.470.10, 1 hit |
HAMAPi | MF_01331_B, Ribosomal_L22_B, 1 hit |
InterProi | View protein in InterPro IPR001063, Ribosomal_L22 IPR018260, Ribosomal_L22/L17_CS IPR036394, Ribosomal_L22/L17_sf IPR005727, Ribosomal_L22_bac/chlpt-type |
PANTHERi | PTHR13501, PTHR13501, 1 hit |
Pfami | View protein in Pfam PF00237, Ribosomal_L22, 1 hit |
SUPFAMi | SSF54843, SSF54843, 1 hit |
TIGRFAMsi | TIGR01044, rplV_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00464, RIBOSOMAL_L22, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RL22_DEIRA | |
Accessioni | Q9RXJ7Primary (citable) accession number: Q9RXJ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families