UniProtKB - Q9R867 (Q9R867_STREE)
DNA gyrase subunit A
gyrA
Functioni
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
UniRule annotationMiscellaneous
Catalytic activityi
- ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationARBA annotation EC:5.6.2.2
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 120 | O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- DNA negative supercoiling activity Source: UniProt
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA topological change Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-bindingUniRule annotationARBA annotation, Isomerase, TopoisomeraseUniRule annotationARBA annotation |
Ligand | ATP-bindingUniRule annotationARBA annotation, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gyrAUniRule annotationImported ORF Names:GM527_04315Imported, GM539_01035Imported, GM545_02690Imported |
Organismi | Streptococcus pneumoniaeImported |
Taxonomic identifieri | 1313 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- chromosome Source: InterPro
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationInteractioni
Subunit structurei
Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.
UniRule annotationStructurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 462 | TOP4cInterPro annotationAdd BLAST | 454 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 524 – 530 | GyrA-boxUniRule annotation | 7 |
Sequence similaritiesi
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_01897, GyrA, 1 hit |
InterProi | View protein in InterPro IPR005743, GyrA IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf IPR002205, Topo_IIA_dom_A |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 6 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MQDKNLVNVN LTKEMKASFI DYAMSVIVAR ALPDVRDGLK PVHRRILYGM
60 70 80 90 100
NELGVTPDKP HKKSARITGD VMGKYHPHGD SSIYEAMVRM AQWWSYRYML
110 120 130 140 150
VDGHGNFGSM DGDSAAAQRY TEARMSKIAL EMLRDINKNT VDFVDNYDAN
160 170 180 190 200
EREPLVLPAR FPNLLVNGAT GIAVGMATNI PPHNLGETID AVKLVMDNPE
210 220 230 240 250
VTTKDLMEVL PGPDFPTGAL VMGKSGIHKA YETGKGSIVL RSRTEIETTK
260 270 280 290 300
TGRERIVVTE FPYMVNKTKV HEHIVRLVQE KRIEGITAVR DESNREGVRF
310 320 330 340 350
VIEVKRDASA NVILNNLFKM TQMQTNFGFN MLAIQNGIPK ILSLRQILDA
360 370 380 390 400
YIEHQKEVVV RRTRFDKEKA EARAHILEGL LIALDHIDEV IRIIRASETD
410 420 430 440 450
AEAQAELMSK FKLSERQSQA ILDMRLRRLT GLERDKIQSE YDDLLALIAD
460 470 480 490 500
LADILAKPER VSQIIKDELD EVKRKFSDKR RTELMVGQIL SLEDEDLIEE
510 520 530 540 550
SDVLITLSNR GYIKRLDQDE FTAQKRGGRG VQGTGVKDDD FVRELVSTST
560 570 580 590 600
HDHLLFFTNK GRVYRLKGYE IPEYGRTAKG LPVVNLLKLD EDESIQTVIN
610 620 630 640 650
VESDRSDDAY LFFTTRHGIV KRTSVKEFAN IRQNGLKALN LKDEDELINV
660 670 680 690 700
LLTEGDMDII IGTKFGYAVR FNQSAVRGMS RIATGVKGVN LREGDTVVGA
710 720 730 740 750
SLITDQDEVL IITEKGYGKR TVATEYPTKG RGGKGMQTAK ITEKNGLLAG
760 770 780 790 800
LMTVQGDEDL MIITDTGVMI RTNLANISQT GRATMGVKVM RLDQDAQIVT
810 820
FTTVAVAEKE EVGTENETEG EA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ175131 Genomic DNA Translation: AAZ92932.1 DQ175136 Genomic DNA Translation: AAZ92937.1 DQ175161 Genomic DNA Translation: AAZ92962.1 DQ175162 Genomic DNA Translation: AAZ92963.1 DQ175167 Genomic DNA Translation: AAZ92968.1 DQ175172 Genomic DNA Translation: AAZ92973.1 AJ005815 Genomic DNA Translation: CAA06715.1 WNHU01000008 Genomic DNA Translation: MTV42567.1 WNHJ01000002 Genomic DNA Translation: MTV62049.1 WNHM01000008 Genomic DNA Translation: MTW26808.1 |
RefSeqi | WP_001152992.1, NZ_WNHW01000024.1 |
Genome annotation databases
EnsemblBacteriai | TKD00611; TKD00611; EZ408_006865 |
PATRICi | fig|1313.5281.peg.348 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ175131 Genomic DNA Translation: AAZ92932.1 DQ175136 Genomic DNA Translation: AAZ92937.1 DQ175161 Genomic DNA Translation: AAZ92962.1 DQ175162 Genomic DNA Translation: AAZ92963.1 DQ175167 Genomic DNA Translation: AAZ92968.1 DQ175172 Genomic DNA Translation: AAZ92973.1 AJ005815 Genomic DNA Translation: CAA06715.1 WNHU01000008 Genomic DNA Translation: MTV42567.1 WNHJ01000002 Genomic DNA Translation: MTV62049.1 WNHM01000008 Genomic DNA Translation: MTW26808.1 |
RefSeqi | WP_001152992.1, NZ_WNHW01000024.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
EnsemblBacteriai | TKD00611; TKD00611; EZ408_006865 |
PATRICi | fig|1313.5281.peg.348 |
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_01897, GyrA, 1 hit |
InterProi | View protein in InterPro IPR005743, GyrA IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf IPR002205, Topo_IIA_dom_A |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 6 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | Q9R867_STREE | |
Accessioni | Q9R867Primary (citable) accession number: Q9R867 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 1, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | January 19, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |