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Protein

Tapasin

Gene

Tapbp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei431May be involved in interaction with TAP1

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Tapasin
Short name:
TPN
Short name:
TPSN
Alternative name(s):
TAP-associated protein
TAP-binding protein
Gene namesi
Name:Tapbp
Synonyms:Tapa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1201689 Tapbp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 416LumenalSequence analysisAdd BLAST393
Transmembranei417 – 437HelicalSequence analysisAdd BLAST21
Topological domaini438 – 465CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000001499124 – 465TapasinAdd BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 94PROSITE-ProRule annotation
Disulfide bondi118Interchain (with C-57 in PDIA3)PROSITE-ProRule annotation
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi318 ↔ 385PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9R233
PeptideAtlasiQ9R233
PRIDEiQ9R233

PTM databases

iPTMnetiQ9R233
PhosphoSitePlusiQ9R233

Expressioni

Gene expression databases

CleanExiMM_TAPBP

Interactioni

Subunit structurei

Interacts with TAP1 and is thus a subunit of the TAP complex. Interaction with TAP1 is TAP2 independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, ERp57 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203968, 5 interactors
ComplexPortaliCPX-531 Tapasin-ERp57 complex
IntActiQ9R233, 3 interactors
MINTiQ9R233
STRINGi10090.ENSMUSP00000025161

Structurei

3D structure databases

ProteinModelPortaliQ9R233
SMRiQ9R233
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini295 – 402Ig-like C1-typeAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi37 – 40Poly-Gly4

Domaini

The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminal region (By similarity).By similarity

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II43 Eukaryota
ENOG4111QPK LUCA
HOGENOMiHOG000231214
HOVERGENiHBG005156
InParanoidiQ9R233
KOiK08058
PhylomeDBiQ9R233

Family and domain databases

Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003597 Ig_C1-set
IPR008056 Tapasin
PfamiView protein in Pfam
PF07654 C1-set, 1 hit
PRINTSiPR01669 TAPASIN
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS00290 IG_MHC, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: Q9R233-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKPLLLLVAV ALGLATVVSV VSAGPEAIEC WFVEDAGGGG LSKKPATLLL
60 70 80 90 100
RHGPRGPPPR PDLDPKLYFK VDDPAGMLLA AFRRYPAGAS APHCEMSRFI
110 120 130 140 150
PFPASAKWAR SLSPEQNCPR ALDGDWLLVS VSSTLFSLSS LLRPQPEPLR
160 170 180 190 200
EPVVITMATV VLTVLTHNPA PRVQLGKDAV LDLRFAYAPS ALEGSPSLDA
210 220 230 240 250
GPPPFGLEWR RQHRGKGHLL LAATPGLAGR MPPAQEKATA FAAWDDDEPW
260 270 280 290 300
GPWTGNGTFW LPAVKPSQEG VYLATVHLPY LQGQVSLELT VHKAPRVSLT
310 320 330 340 350
PAPVVWAAPG EAPPELLCLA SHFFPAEGLE VKWELRGGPG GSSRKVEGKT
360 370 380 390 400
WLSTIRHHSD GSVSQSGHLQ LPPVTAKQHG VHYVCRVYHS SLPASGRSAD
410 420 430 440 450
VTLEVAGFSG PSIEDGIGLF LSAFLLLGLL KVLGWLAAYW TIPEVSKEKA
460
TAASLTIPRN SKKSQ
Length:465
Mass (Da):49,736
Last modified:October 3, 2003 - v2
Checksum:iDCADF445A1437C3C
GO
Isoform Short (identifier: Q9R233-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     407-436: Missing.

Show »
Length:435
Mass (Da):46,621
Checksum:iEF81A50547D8A004
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3TCU5Q3TCU5_MOUSE
Tapasin
Tapbp
466Annotation score:
G3UZZ2G3UZZ2_MOUSE
Tapasin
Tapbp
119Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17V → F in AAC61676 (PubMed:9716645).Curated1
Sequence conflicti274A → G in AAC61676 (PubMed:9716645).Curated1
Sequence conflicti294A → G in AAC61676 (PubMed:9716645).Curated1
Sequence conflicti326A → S in AAD17964 (PubMed:10085102).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002578407 – 436Missing in isoform Short. CuratedAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106278 mRNA Translation: AAD17964.1
AF110520 Genomic DNA Translation: AAC97973.1
AF100956 Genomic DNA Translation: AAC69893.1
AF043943 mRNA Translation: AAC61676.1
AJ316612 Genomic DNA Translation: CAC69993.1
AJ316613 mRNA Translation: CAC70721.2
RefSeqiNP_033344.1, NM_009318.2 [Q9R233-1]
UniGeneiMm.154457

Genome annotation databases

GeneIDi21356
KEGGimmu:21356
UCSCiuc008cae.1 mouse [Q9R233-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106278 mRNA Translation: AAD17964.1
AF110520 Genomic DNA Translation: AAC97973.1
AF100956 Genomic DNA Translation: AAC69893.1
AF043943 mRNA Translation: AAC61676.1
AJ316612 Genomic DNA Translation: CAC69993.1
AJ316613 mRNA Translation: CAC70721.2
RefSeqiNP_033344.1, NM_009318.2 [Q9R233-1]
UniGeneiMm.154457

3D structure databases

ProteinModelPortaliQ9R233
SMRiQ9R233
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203968, 5 interactors
ComplexPortaliCPX-531 Tapasin-ERp57 complex
IntActiQ9R233, 3 interactors
MINTiQ9R233
STRINGi10090.ENSMUSP00000025161

PTM databases

iPTMnetiQ9R233
PhosphoSitePlusiQ9R233

Proteomic databases

PaxDbiQ9R233
PeptideAtlasiQ9R233
PRIDEiQ9R233

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21356
KEGGimmu:21356
UCSCiuc008cae.1 mouse [Q9R233-1]

Organism-specific databases

CTDi6892
MGIiMGI:1201689 Tapbp

Phylogenomic databases

eggNOGiENOG410II43 Eukaryota
ENOG4111QPK LUCA
HOGENOMiHOG000231214
HOVERGENiHBG005156
InParanoidiQ9R233
KOiK08058
PhylomeDBiQ9R233

Miscellaneous databases

ChiTaRSiTapbp mouse
PROiPR:Q9R233
SOURCEiSearch...

Gene expression databases

CleanExiMM_TAPBP

Family and domain databases

Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003006 Ig/MHC_CS
IPR003597 Ig_C1-set
IPR008056 Tapasin
PfamiView protein in Pfam
PF07654 C1-set, 1 hit
PRINTSiPR01669 TAPASIN
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS00290 IG_MHC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPSN_MOUSE
AccessioniPrimary (citable) accession number: Q9R233
Secondary accession number(s): O70317
, Q91YE0, Q91YE1, Q9QWT7, Q9Z1W3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 3, 2003
Last modified: June 20, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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