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UniProtKB - Q9R1Z7 (PTPS_MOUSE)
Protein
6-pyruvoyl tetrahydrobiopterin synthase
Gene
Pts
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
1 PublicationMiscellaneous
The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.
Catalytic activityi
- 7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + H+ + triphosphate1 PublicationEC:4.2.3.121 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Kineticsi
kcat is 0.27 (-1) with dihydroneopterin triphosphate as substrate.1 Publication
- KM=10.6 µM for dihydroneopterin triphosphate1 Publication
: tetrahydrobiopterin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate. This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 23 | ZincPROSITE-ProRule annotation | 1 | |
Active sitei | 42 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 48 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 50 | ZincPROSITE-ProRule annotation | 1 | |
Active sitei | 89 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 133 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 6-pyruvoyltetrahydropterin synthase activity Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- tetrahydrobiopterin biosynthetic process Source: MGI
Keywordsi
Molecular function | Lyase |
Biological process | Tetrahydrobiopterin biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation |
UniPathwayi | UPA00849;UER00819 |
Names & Taxonomyi
Protein namesi | Recommended name: 6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.121 Publication)Short name: PTP synthase Short name: PTPS |
Gene namesi | Name:PtsImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1338783, Pts |
VEuPathDBi | HostDB:ENSMUSG00000032067 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057915 | 1 – 144 | 6-pyruvoyl tetrahydrobiopterin synthaseAdd BLAST | 144 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | PhosphoserineBy similarity | 1 | |
Modified residuei | 27 | PhosphoserineCombined sources | 1 | |
Modified residuei | 127 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Phosphorylation of Ser-18 is required for maximal enzyme activity.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9R1Z7 |
jPOSTi | Q9R1Z7 |
MaxQBi | Q9R1Z7 |
PaxDbi | Q9R1Z7 |
PeptideAtlasi | Q9R1Z7 |
PRIDEi | Q9R1Z7 |
ProteomicsDBi | 301953 |
PTM databases
iPTMneti | Q9R1Z7 |
PhosphoSitePlusi | Q9R1Z7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032067, Expressed in brown adipose tissue and 310 other tissues |
ExpressionAtlasi | Q9R1Z7, baseline and differential |
Genevisiblei | Q9R1Z7, MM |
Interactioni
Subunit structurei
Homodimer (PubMed:9894812). Homohexamer formed of two homotrimers in a head to head fashion.
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202512, 4 interactors |
IntActi | Q9R1Z7, 4 interactors |
MINTi | Q9R1Z7 |
STRINGi | 10090.ENSMUSP00000034570 |
Miscellaneous databases
RNActi | Q9R1Z7, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the PTPS family.Curated
Phylogenomic databases
eggNOGi | KOG4105, Eukaryota |
GeneTreei | ENSGT00390000002752 |
HOGENOMi | CLU_111016_2_0_1 |
InParanoidi | Q9R1Z7 |
OMAi | NIAVVIW |
OrthoDBi | 1502673at2759 |
PhylomeDBi | Q9R1Z7 |
TreeFami | TF105796 |
Family and domain databases
Gene3Di | 3.30.479.10, 1 hit |
InterProi | View protein in InterPro IPR007115, 6-PTP_synth/QueD IPR038418, 6-PTP_synth/QueD_sf IPR022470, PTPS_Cys_AS IPR022469, PTPS_His_AS |
PANTHERi | PTHR12589, PTHR12589, 1 hit |
Pfami | View protein in Pfam PF01242, PTPS, 1 hit |
PIRSFi | PIRSF006113, PTP_synth, 1 hit |
TIGRFAMsi | TIGR00039, 6PTHBS, 1 hit |
PROSITEi | View protein in PROSITE PS00987, PTPS_1, 1 hit PS00988, PTPS_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9R1Z7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAAGDLRRR ARLSRLVSFS ASHRLHSPSL SDEENLRVFG KCNNPNGHGH
60 70 80 90 100
NYKVVVTVHG EIDPVTGMVM NLTDLKEYME EAIMKPLDHK NLDLDVPYFA
110 120 130 140
DAVSTTENVA VYIWESLQKL LPVGALYKVK VFETDNNIVV YKGE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SRN0 | A0A1L1SRN0_MOUSE | 6-pyruvoyl tetrahydrobiopterin synt... | Pts | 77 | Annotation score: | ||
A0A1L1SQY6 | A0A1L1SQY6_MOUSE | 6-pyruvoyl tetrahydrobiopterin synt... | Pts | 23 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | D → G in AAD15827 (PubMed:9894812).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061880, AF061878, AF061879 Genomic DNA Translation: AAD15827.1 AF043225 mRNA Translation: AAD02249.1 AK002614 mRNA Translation: BAB22231.1 AK146987 mRNA Translation: BAE27590.1 BC029013 mRNA Translation: AAH29013.1 |
CCDSi | CCDS23164.1 |
RefSeqi | NP_035350.1, NM_011220.2 |
Genome annotation databases
Ensembli | ENSMUST00000034570; ENSMUSP00000034570; ENSMUSG00000032067 |
GeneIDi | 19286 |
KEGGi | mmu:19286 |
UCSCi | uc009pjp.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061880, AF061878, AF061879 Genomic DNA Translation: AAD15827.1 AF043225 mRNA Translation: AAD02249.1 AK002614 mRNA Translation: BAB22231.1 AK146987 mRNA Translation: BAE27590.1 BC029013 mRNA Translation: AAH29013.1 |
CCDSi | CCDS23164.1 |
RefSeqi | NP_035350.1, NM_011220.2 |
3D structure databases
SMRi | Q9R1Z7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202512, 4 interactors |
IntActi | Q9R1Z7, 4 interactors |
MINTi | Q9R1Z7 |
STRINGi | 10090.ENSMUSP00000034570 |
PTM databases
iPTMneti | Q9R1Z7 |
PhosphoSitePlusi | Q9R1Z7 |
Proteomic databases
EPDi | Q9R1Z7 |
jPOSTi | Q9R1Z7 |
MaxQBi | Q9R1Z7 |
PaxDbi | Q9R1Z7 |
PeptideAtlasi | Q9R1Z7 |
PRIDEi | Q9R1Z7 |
ProteomicsDBi | 301953 |
Protocols and materials databases
Antibodypediai | 780, 210 antibodies from 27 providers |
DNASUi | 19286 |
Genome annotation databases
Ensembli | ENSMUST00000034570; ENSMUSP00000034570; ENSMUSG00000032067 |
GeneIDi | 19286 |
KEGGi | mmu:19286 |
UCSCi | uc009pjp.1, mouse |
Organism-specific databases
CTDi | 5805 |
MGIi | MGI:1338783, Pts |
VEuPathDBi | HostDB:ENSMUSG00000032067 |
Phylogenomic databases
eggNOGi | KOG4105, Eukaryota |
GeneTreei | ENSGT00390000002752 |
HOGENOMi | CLU_111016_2_0_1 |
InParanoidi | Q9R1Z7 |
OMAi | NIAVVIW |
OrthoDBi | 1502673at2759 |
PhylomeDBi | Q9R1Z7 |
TreeFami | TF105796 |
Enzyme and pathway databases
UniPathwayi | UPA00849;UER00819 |
Reactomei | R-MMU-1474151, Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation |
Miscellaneous databases
BioGRID-ORCSi | 19286, 0 hits in 64 CRISPR screens |
ChiTaRSi | Pts, mouse |
PROi | PR:Q9R1Z7 |
RNActi | Q9R1Z7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032067, Expressed in brown adipose tissue and 310 other tissues |
ExpressionAtlasi | Q9R1Z7, baseline and differential |
Genevisiblei | Q9R1Z7, MM |
Family and domain databases
Gene3Di | 3.30.479.10, 1 hit |
InterProi | View protein in InterPro IPR007115, 6-PTP_synth/QueD IPR038418, 6-PTP_synth/QueD_sf IPR022470, PTPS_Cys_AS IPR022469, PTPS_His_AS |
PANTHERi | PTHR12589, PTHR12589, 1 hit |
Pfami | View protein in Pfam PF01242, PTPS, 1 hit |
PIRSFi | PIRSF006113, PTP_synth, 1 hit |
TIGRFAMsi | TIGR00039, 6PTHBS, 1 hit |
PROSITEi | View protein in PROSITE PS00987, PTPS_1, 1 hit PS00988, PTPS_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTPS_MOUSE | |
Accessioni | Q9R1Z7Primary (citable) accession number: Q9R1Z7 Secondary accession number(s): Q3UIB6, Q9Z2N2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 2002 |
Last sequence update: | November 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families