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UniProtKB - Q9R1T5 (ACY2_RAT)
Protein
Aspartoacylase
Gene
Aspa
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter (By similarity).
By similarityCatalytic activityi
- EC:3.5.1.151 Publication
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 20 | Zinc | 1 | |
Metal bindingi | 23 | Zinc | 1 | |
Binding sitei | 62 | SubstrateBy similarity | 1 | |
Metal bindingi | 115 | Zinc | 1 | |
Active sitei | 177 | By similarity | 1 | |
Binding sitei | 177 | SubstrateBy similarity | 1 | |
Binding sitei | 287 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- aspartoacylase activity Source: RGD
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: GO_Central
- hydrolase activity, acting on ester bonds Source: InterPro
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- central nervous system myelination Source: RGD
- positive regulation of oligodendrocyte differentiation Source: RGD
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.5.1.15, 5301 |
Reactomei | R-RNO-8963693, Aspartate and asparagine metabolism |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Aspa |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621693, Aspa |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000216874 | 1 – 312 | AspartoacylaseAdd BLAST | 312 |
Proteomic databases
PaxDbi | Q9R1T5 |
PRIDEi | Q9R1T5 |
PTM databases
iPTMneti | Q9R1T5 |
PhosphoSitePlusi | Q9R1T5 |
SwissPalmi | Q9R1T5 |
Expressioni
Tissue specificityi
Detected in kidney proximal tubule cells (at protein level).1 Publication
Gene expression databases
Bgeei | ENSRNOG00000019659, Expressed in kidney and 22 other tissues |
ExpressionAtlasi | Q9R1T5, baseline and differential |
Genevisiblei | Q9R1T5, RN |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Q9R1T5
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-15617537,EBI-15617537 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-60794N |
STRINGi | 10116.ENSRNOP00000050760 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9R1T5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9R1T5 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 69 – 70 | Substrate bindingBy similarity | 2 | |
Regioni | 163 – 167 | Substrate bindingBy similarity | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QRAK, Eukaryota |
GeneTreei | ENSGT00390000001189 |
HOGENOMi | CLU_083292_0_0_1 |
InParanoidi | Q9R1T5 |
OrthoDBi | 693004at2759 |
Family and domain databases
HAMAPi | MF_00704, Aspartoacylase, 1 hit |
InterProi | View protein in InterPro IPR016708, Aspartoacylase IPR007036, Aste_AspA |
Pfami | View protein in Pfam PF04952, AstE_AspA, 1 hit |
PIRSFi | PIRSF018001, Aspartoacylase, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9R1T5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSCVAEEPI KKIAIFGGTH GNELTGVFLV THWLKNGAEV HRAGLEVKPF
60 70 80 90 100
ITNPRAVEKC TRYIDCDLNR VFDLENLSKE MSEDLPYEVR RAQEINHLFG
110 120 130 140 150
PKNSDDAYDV VFDLHNTTSN MGCTLILEDS RNDFLIQMFH YIKTCMAPLP
160 170 180 190 200
CSVYLIEHPS LKYATTRSIA KYPVGIEVGP QPHGVLRADI LDQMRRMLKH
210 220 230 240 250
ALDFIQRFNE GKEFPPCAID VYKIMEKVDY PRNESGDVAA VIHPNLQDQD
260 270 280 290 300
WKPLHPGDPV FVSLDGKVIP LGGDCTVYPV FVNEAAYYEK KEAFAKTTKL
310
TLNAKSIRST LH
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHY8 | A0A0H2UHY8_RAT | Aspartoacylase | Aspa | 314 | Annotation score: | ||
A0A1W2Q6K0 | A0A1W2Q6K0_RAT | Aspartoacylase | Aspa | 262 | Annotation score: | ||
A0A1W2Q5Z4 | A0A1W2Q5Z4_RAT | Aspartoacylase | Aspa | 179 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 103 | N → D in BAA82801 (Ref. 1) Curated | 1 | |
Sequence conflicti | 113 | D → V in BAA82801 (Ref. 1) Curated | 1 | |
Sequence conflicti | 182 | P → H in BAA82801 (Ref. 1) Curated | 1 | |
Sequence conflicti | 247 | Q → QLQ in BAA82801 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB023432 mRNA Translation: BAA82801.1 BC078813 mRNA Translation: AAH78813.1 BC078814 mRNA Translation: AAH78814.1 |
RefSeqi | NP_077375.1, NM_024399.1 XP_006246877.1, XM_006246815.3 |
Genome annotation databases
Ensembli | ENSRNOT00000084432; ENSRNOP00000072876; ENSRNOG00000019659 ENSRNOT00000093230; ENSRNOP00000076091; ENSRNOG00000019659 |
GeneIDi | 79251 |
KEGGi | rno:79251 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB023432 mRNA Translation: BAA82801.1 BC078813 mRNA Translation: AAH78813.1 BC078814 mRNA Translation: AAH78814.1 |
RefSeqi | NP_077375.1, NM_024399.1 XP_006246877.1, XM_006246815.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GU2 | X-ray | 1.80 | A/B | 2-312 | [»] | |
2Q4Z | X-ray | 1.80 | A/B | 2-312 | [»] | |
SMRi | Q9R1T5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-60794N |
STRINGi | 10116.ENSRNOP00000050760 |
PTM databases
iPTMneti | Q9R1T5 |
PhosphoSitePlusi | Q9R1T5 |
SwissPalmi | Q9R1T5 |
Proteomic databases
PaxDbi | Q9R1T5 |
PRIDEi | Q9R1T5 |
Genome annotation databases
Ensembli | ENSRNOT00000084432; ENSRNOP00000072876; ENSRNOG00000019659 ENSRNOT00000093230; ENSRNOP00000076091; ENSRNOG00000019659 |
GeneIDi | 79251 |
KEGGi | rno:79251 |
Organism-specific databases
CTDi | 443 |
RGDi | 621693, Aspa |
Phylogenomic databases
eggNOGi | ENOG502QRAK, Eukaryota |
GeneTreei | ENSGT00390000001189 |
HOGENOMi | CLU_083292_0_0_1 |
InParanoidi | Q9R1T5 |
OrthoDBi | 693004at2759 |
Enzyme and pathway databases
BRENDAi | 3.5.1.15, 5301 |
Reactomei | R-RNO-8963693, Aspartate and asparagine metabolism |
Miscellaneous databases
EvolutionaryTracei | Q9R1T5 |
PROi | PR:Q9R1T5 |
Gene expression databases
Bgeei | ENSRNOG00000019659, Expressed in kidney and 22 other tissues |
ExpressionAtlasi | Q9R1T5, baseline and differential |
Genevisiblei | Q9R1T5, RN |
Family and domain databases
HAMAPi | MF_00704, Aspartoacylase, 1 hit |
InterProi | View protein in InterPro IPR016708, Aspartoacylase IPR007036, Aste_AspA |
Pfami | View protein in Pfam PF04952, AstE_AspA, 1 hit |
PIRSFi | PIRSF018001, Aspartoacylase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACY2_RAT | |
Accessioni | Q9R1T5Primary (citable) accession number: Q9R1T5 Secondary accession number(s): Q6AZ03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2003 |
Last sequence update: | January 9, 2007 | |
Last modified: | June 2, 2021 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families