UniProtKB - Q9R1R2 (TRIM3_MOUSE)
Protein
Tripartite motif-containing protein 3
Gene
Trim3
Organism
Mus musculus (Mouse)
Status
Functioni
Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Positively regulates motility of microtubule-dependent motor protein KIF21B (PubMed:24086586).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 22 – 63 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 110 – 151 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- identical protein binding Source: MGI
- ubiquitin protein ligase activity Source: MGI
- ubiquitin-protein transferase activity Source: FlyBase
- zinc ion binding Source: InterPro
GO - Biological processi
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein polyubiquitination Source: GO_Central
- protein transport Source: UniProtKB-KW
- protein ubiquitination Source: FlyBase
Keywordsi
Biological process | Protein transport, Transport |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Tripartite motif-containing protein 3Alternative name(s): RING finger protein 22 RING finger protein HAC1 |
Gene namesi | Name:Trim3 Synonyms:Hac1, Rnf22 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1860040, Trim3 |
Subcellular locationi
Golgi apparatus
- trans-Golgi network 1 Publication
Endosome
- Early endosome By similarity
Other locations
Endosome
- early endosome Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: MGI
- dendrite Source: UniProtKB-SubCell
- glutamatergic synapse Source: MGI
- postsynapse Source: MGI
Keywords - Cellular componenti
Cell projection, Cytoplasm, Endosome, Golgi apparatusPathology & Biotechi
Disruption phenotypei
No visible phenotype.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000056198 | 2 – 744 | Tripartite motif-containing protein 3Add BLAST | 743 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 7 | PhosphoserineCombined sources | 1 | |
Modified residuei | 427 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q9R1R2 |
MaxQBi | Q9R1R2 |
PaxDbi | Q9R1R2 |
PRIDEi | Q9R1R2 |
PTM databases
iPTMneti | Q9R1R2 |
PhosphoSitePlusi | Q9R1R2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000036989, Expressed in cerebellar cortex and 288 other tissues |
Genevisiblei | Q9R1R2, MM |
Interactioni
Subunit structurei
Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4) (By similarity).
Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3 (By similarity).
Interacts with ZFYVE28/LST2 (By similarity).
Interacts with KIF21B (PubMed:24086586).
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207763, 16 interactors |
IntActi | Q9R1R2, 11 interactors |
MINTi | Q9R1R2 |
STRINGi | 10090.ENSMUSP00000053384 |
Miscellaneous databases
RNActi | Q9R1R2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 317 – 418 | FilaminAdd BLAST | 102 | |
Repeati | 473 – 516 | NHL 1Add BLAST | 44 | |
Repeati | 520 – 563 | NHL 2Add BLAST | 44 | |
Repeati | 564 – 605 | NHL 3Add BLAST | 42 | |
Repeati | 609 – 652 | NHL 4Add BLAST | 44 | |
Repeati | 656 – 699 | NHL 5Add BLAST | 44 | |
Repeati | 700 – 743 | NHL 6Add BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 290 | Interaction with KIF21B1 PublicationAdd BLAST | 289 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 153 – 224 | Sequence analysisAdd BLAST | 72 |
Domaini
The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.By similarity
Sequence similaritiesi
Belongs to the TRIM/RBCC family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 22 – 63 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 | |
Zinc fingeri | 110 – 151 | B box-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Coiled coil, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000158173 |
HOGENOMi | CLU_008645_5_0_1 |
InParanoidi | Q9R1R2 |
OMAi | EPCETAM |
OrthoDBi | 489543at2759 |
PhylomeDBi | Q9R1R2 |
TreeFami | TF331018 |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit |
Gene3Di | 2.120.10.30, 2 hits 2.60.40.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR003649, Bbox_C IPR017868, Filamin/ABP280_repeat-like IPR001298, Filamin/ABP280_rpt IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR001258, NHL_repeat IPR013017, NHL_repeat_subgr IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00630, Filamin, 1 hit PF01436, NHL, 6 hits PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
SMARTi | View protein in SMART SM00502, BBC, 1 hit SM00336, BBOX, 1 hit SM00557, IG_FLMN, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50194, FILAMIN_REPEAT, 1 hit PS51125, NHL, 6 hits PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9R1R2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKREDSPGP EVQPMDKQFL VCSICLDRYR CPKVLPCLHT FCERCLQNYI
60 70 80 90 100
PPQSLTLSCP VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPEGAHDPE
110 120 130 140 150
DPHPLSAVAG RPLSCPNHEG KTMEFYCEAC ETAMCGECRA GEHREHGTVL
160 170 180 190 200
LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI ALVGGISQQL QERKAEALAQ
210 220 230 240 250
ISAAFEDLEQ ALQQRKQALV SDLESICGAK QKVLQTQLDT LRQGQEHIGS
260 270 280 290 300
SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL
310 320 330 340 350
EVDGLRRSVL NLGALLTTSA TAHETVATGE GLRQALVGQP ASLTVTTKDK
360 370 380 390 400
DGRLVRTGSA ELCAEITGPD GVRLAVPVVD HKNGTYELVY TARTEGDLLL
410 420 430 440 450
SVLLYGQPVR GSPFRVRALR PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV
460 470 480 490 500
RRPSSMYSTG GKRKDNPIED ELVFRVGSRG REKGEFTNLQ GVSAASSGRI
510 520 530 540 550
VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA VDTNGDIIVA
560 570 580 590 600
DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF
610 620 630 640 650
TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS
660 670 680 690 700
ADGEFLFKFG SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG
710 720 730 740
SFLSYINTSA EPLYGPQGLA LTSDGHVVVA DAGNHCFKAY RYLQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TDT0 | Q3TDT0_MOUSE | Tripartite motif-containing protein... | Trim3 | 717 | Annotation score: | ||
D3Z7V9 | D3Z7V9_MOUSE | Tripartite motif-containing protein... | Trim3 | 287 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030912 mRNA Translation: BAA83343.1 AF220019 mRNA Translation: AAG53473.1 AK019165 mRNA Translation: BAB31580.1 AK144674 mRNA Translation: BAE26003.1 BC034263 mRNA Translation: AAH34263.1 |
CCDSi | CCDS21655.1 |
RefSeqi | NP_001272799.1, NM_001285870.1 NP_001272800.1, NM_001285871.1 NP_001272802.1, NM_001285873.1 NP_061368.1, NM_018880.3 |
Genome annotation databases
Ensembli | ENSMUST00000057525; ENSMUSP00000053384; ENSMUSG00000036989 ENSMUST00000106789; ENSMUSP00000102401; ENSMUSG00000036989 ENSMUST00000147044; ENSMUSP00000114822; ENSMUSG00000036989 |
GeneIDi | 55992 |
KEGGi | mmu:55992 |
UCSCi | uc009iym.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB030912 mRNA Translation: BAA83343.1 AF220019 mRNA Translation: AAG53473.1 AK019165 mRNA Translation: BAB31580.1 AK144674 mRNA Translation: BAE26003.1 BC034263 mRNA Translation: AAH34263.1 |
CCDSi | CCDS21655.1 |
RefSeqi | NP_001272799.1, NM_001285870.1 NP_001272800.1, NM_001285871.1 NP_001272802.1, NM_001285873.1 NP_061368.1, NM_018880.3 |
3D structure databases
SMRi | Q9R1R2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207763, 16 interactors |
IntActi | Q9R1R2, 11 interactors |
MINTi | Q9R1R2 |
STRINGi | 10090.ENSMUSP00000053384 |
PTM databases
iPTMneti | Q9R1R2 |
PhosphoSitePlusi | Q9R1R2 |
Proteomic databases
jPOSTi | Q9R1R2 |
MaxQBi | Q9R1R2 |
PaxDbi | Q9R1R2 |
PRIDEi | Q9R1R2 |
Protocols and materials databases
Antibodypediai | 11249, 239 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000057525; ENSMUSP00000053384; ENSMUSG00000036989 ENSMUST00000106789; ENSMUSP00000102401; ENSMUSG00000036989 ENSMUST00000147044; ENSMUSP00000114822; ENSMUSG00000036989 |
GeneIDi | 55992 |
KEGGi | mmu:55992 |
UCSCi | uc009iym.2, mouse |
Organism-specific databases
CTDi | 10612 |
MGIi | MGI:1860040, Trim3 |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000158173 |
HOGENOMi | CLU_008645_5_0_1 |
InParanoidi | Q9R1R2 |
OMAi | EPCETAM |
OrthoDBi | 489543at2759 |
PhylomeDBi | Q9R1R2 |
TreeFami | TF331018 |
Miscellaneous databases
BioGRID-ORCSi | 55992, 1 hit in 17 CRISPR screens |
ChiTaRSi | Trim3, mouse |
PROi | PR:Q9R1R2 |
RNActi | Q9R1R2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036989, Expressed in cerebellar cortex and 288 other tissues |
Genevisiblei | Q9R1R2, MM |
Family and domain databases
CDDi | cd00021, BBOX, 1 hit |
Gene3Di | 2.120.10.30, 2 hits 2.60.40.10, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR003649, Bbox_C IPR017868, Filamin/ABP280_repeat-like IPR001298, Filamin/ABP280_rpt IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR001258, NHL_repeat IPR013017, NHL_repeat_subgr IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00630, Filamin, 1 hit PF01436, NHL, 6 hits PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
SMARTi | View protein in SMART SM00502, BBC, 1 hit SM00336, BBOX, 1 hit SM00557, IG_FLMN, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS50194, FILAMIN_REPEAT, 1 hit PS51125, NHL, 6 hits PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRIM3_MOUSE | |
Accessioni | Q9R1R2Primary (citable) accession number: Q9R1R2 Secondary accession number(s): Q3UMU5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 6, 2002 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families