UniProtKB - Q9R1P0 (PSA4_MOUSE)
Protein
Proteasome subunit alpha type-4
Gene
Psma4
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).2 Publications
GO - Molecular functioni
- endopeptidase activity Source: GO_Central
- threonine-type endopeptidase activity Source: InterPro
GO - Biological processi
- proteasomal protein catabolic process Source: GO_Central
- proteasomal ubiquitin-independent protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8941858, Regulation of RUNX3 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | T01.973 |
Names & Taxonomyi
Protein namesi | Recommended name: Proteasome subunit alpha type-4Alternative name(s): Macropain subunit C9 Multicatalytic endopeptidase complex subunit C9 Proteasome component C9 Proteasome subunit L |
Gene namesi | Name:Psma4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1347060, Psma4 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: MGI
- P-body Source: UniProtKB
- proteasome complex Source: MGI
- proteasome core complex Source: UniProtKB
- proteasome core complex, alpha-subunit complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124105 | 1 – 261 | Proteasome subunit alpha type-4Add BLAST | 261 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 75 | PhosphoserineBy similarity | 1 | |
Modified residuei | 127 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 173 | PhosphoserineBy similarity | 1 | |
Modified residuei | 176 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3424 |
EPDi | Q9R1P0 |
jPOSTi | Q9R1P0 |
MaxQBi | Q9R1P0 |
PaxDbi | Q9R1P0 |
PeptideAtlasi | Q9R1P0 |
PRIDEi | Q9R1P0 |
PTM databases
iPTMneti | Q9R1P0 |
PhosphoSitePlusi | Q9R1P0 |
SwissPalmi | Q9R1P0 |
Expressioni
Tissue specificityi
Detected in liver (at protein level).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000032301, Expressed in maxillary prominence and 290 other tissues |
ExpressionAtlasi | Q9R1P0, baseline and differential |
Genevisiblei | Q9R1P0, MM |
Interactioni
Subunit structurei
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7 (PubMed:16857966, PubMed:22341445).
2 PublicationsProtein-protein interaction databases
BioGRIDi | 204992, 58 interactors |
CORUMi | Q9R1P0 |
IntActi | Q9R1P0, 9 interactors |
MINTi | Q9R1P0 |
STRINGi | 10090.ENSMUSP00000034848 |
Miscellaneous databases
RNActi | Q9R1P0, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9R1P0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase T1A family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0178, Eukaryota |
GeneTreei | ENSGT00550000074827 |
InParanoidi | Q9R1P0 |
OMAi | CNEKQRY |
OrthoDBi | 1222564at2759 |
PhylomeDBi | Q9R1P0 |
TreeFami | TF106209 |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR000426, Proteasome_asu_N IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9R1P0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR
60 70 80 90 100
NIHKLLDEVF FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ
110 120 130 140 150
YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS
160 170 180 190 200
DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK EGEMTLKSAL ALAVKVLNKT
210 220 230 240 250
MDVSKLSAEK VEIATLTRES GKTVIRVLKQ KEVEQLIKKH EEEEAKAERE
260
KKEKEQREKD K
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q0X0 | E9Q0X0_MOUSE | Proteasome subunit alpha type | Psma4 | 139 | Annotation score: | ||
E9PW69 | E9PW69_MOUSE | Proteasome subunit alpha type | Psma4 | 210 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060093 mRNA Translation: AAD50538.1 AK085937 mRNA Translation: BAC39573.1 AK168150 mRNA Translation: BAE40115.1 BC001982 mRNA Translation: AAH01982.1 |
CCDSi | CCDS40643.1 |
RefSeqi | NP_036096.1, NM_011966.3 XP_006511261.1, XM_006511198.3 |
Genome annotation databases
Ensembli | ENSMUST00000034848; ENSMUSP00000034848; ENSMUSG00000032301 |
GeneIDi | 26441 |
KEGGi | mmu:26441 |
UCSCi | uc009pru.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060093 mRNA Translation: AAD50538.1 AK085937 mRNA Translation: BAC39573.1 AK168150 mRNA Translation: BAE40115.1 BC001982 mRNA Translation: AAH01982.1 |
CCDSi | CCDS40643.1 |
RefSeqi | NP_036096.1, NM_011966.3 XP_006511261.1, XM_006511198.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3UNB | X-ray | 2.90 | B/P/d/r | 1-261 | [»] | |
3UNE | X-ray | 3.20 | B/P/d/r | 1-261 | [»] | |
3UNF | X-ray | 2.90 | B/P | 1-261 | [»] | |
3UNH | X-ray | 3.20 | B/P | 1-261 | [»] | |
SMRi | Q9R1P0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204992, 58 interactors |
CORUMi | Q9R1P0 |
IntActi | Q9R1P0, 9 interactors |
MINTi | Q9R1P0 |
STRINGi | 10090.ENSMUSP00000034848 |
Protein family/group databases
MEROPSi | T01.973 |
PTM databases
iPTMneti | Q9R1P0 |
PhosphoSitePlusi | Q9R1P0 |
SwissPalmi | Q9R1P0 |
Proteomic databases
CPTACi | non-CPTAC-3424 |
EPDi | Q9R1P0 |
jPOSTi | Q9R1P0 |
MaxQBi | Q9R1P0 |
PaxDbi | Q9R1P0 |
PeptideAtlasi | Q9R1P0 |
PRIDEi | Q9R1P0 |
Protocols and materials databases
Antibodypediai | 27657, 319 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034848; ENSMUSP00000034848; ENSMUSG00000032301 |
GeneIDi | 26441 |
KEGGi | mmu:26441 |
UCSCi | uc009pru.1, mouse |
Organism-specific databases
CTDi | 5685 |
MGIi | MGI:1347060, Psma4 |
Phylogenomic databases
eggNOGi | KOG0178, Eukaryota |
GeneTreei | ENSGT00550000074827 |
InParanoidi | Q9R1P0 |
OMAi | CNEKQRY |
OrthoDBi | 1222564at2759 |
PhylomeDBi | Q9R1P0 |
TreeFami | TF106209 |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8941858, Regulation of RUNX3 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 26441, 8 hits in 18 CRISPR screens |
ChiTaRSi | Psma4, mouse |
PROi | PR:Q9R1P0 |
RNActi | Q9R1P0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032301, Expressed in maxillary prominence and 290 other tissues |
ExpressionAtlasi | Q9R1P0, baseline and differential |
Genevisiblei | Q9R1P0, MM |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR000426, Proteasome_asu_N IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSA4_MOUSE | |
Accessioni | Q9R1P0Primary (citable) accession number: Q9R1P0 Secondary accession number(s): Q3THT1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries